FastQCFastQC Report
Thu 2 Feb 2017
SRR4062514_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062514_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603966
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35410.22076527806699145No Hit
GTACATGGAAGCAGTGGTATCAACG26220.16346979923514587No Hit
TCCATGTACTCTGCGTTGATACCAC22570.14071370590149668No Hit
TATCAACGCAGAGTACTTTTTTTTT21570.13447915978268865No Hit
GCGCAAGACGGACCAGAGCGAAAGC21380.13329459602011512No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21120.13167361402922506No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20660.1288057228145734No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19340.1205761219377468No Hit
GAATAGGACCGCGGTTCTATTTTGT19230.11989032186467793No Hit
GAACTACGACGGTATCTGATCGTCT18820.11733415795596663No Hit
GATTAAGAGGGACGGCCGGGGGCAT18460.11508972135319577No Hit
GAATAACGCCGCCGCATCGCCAGTC17880.11147368460428712No Hit
GTATCTGATCGTCTTCGAACCTCCG17060.10636135678686456No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16950.10567555671379568No Hit
GGGTAGGCACACGCTGAGCCAGTCA16430.10243359273201552No Hit
GTCCTATTCCATTATTCCTAGCTGC16070.10018915612924463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA801.2855162E-714.25269315
AGGCCCG3400.013.69205410
CGGTTCT3950.013.46921812
GCTCGTA1205.0931703E-1113.4591959
TACGCGT500.001500622513.2987775
TTATACC500.001502742313.2962854
ACGGTAT3550.012.5782719
TAGAGTG2350.012.5307615
ACGCGCG1301.9826984E-1012.42426113
CGGTCCA3100.012.2588510
GGTTCTA4500.012.24561213
GTCTTAG1251.4260877E-912.1516711
TCCTATA802.8848131E-511.8676082
TCTCGTC1157.066774E-811.5659612
GCGCGCT1406.7848305E-1011.53789415
TGAGTTA1701.0913936E-1111.17718712
GCATCGC2900.011.13899314
CTCGTAG1451.2150849E-911.13864510
GACGGTA3950.011.0640018
CGTCGTA2750.011.05525410
Produced by FastQC (version 0.11.5)