##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062514_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1603966
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085739972044294	32.0	32.0	32.0	32.0	32.0
2	30.658370563964574	32.0	32.0	32.0	32.0	32.0
3	30.642858389766367	32.0	32.0	32.0	32.0	32.0
4	30.73338462286607	32.0	32.0	32.0	32.0	32.0
5	30.474480132371884	32.0	32.0	32.0	32.0	32.0
6	34.127240228284144	36.0	36.0	36.0	32.0	36.0
7	33.983615612799774	36.0	36.0	36.0	32.0	36.0
8	33.97248133688619	36.0	36.0	36.0	32.0	36.0
9	34.19274847471829	36.0	36.0	36.0	32.0	36.0
10	33.76541772082451	36.0	36.0	36.0	32.0	36.0
11	34.17287523550998	36.0	36.0	36.0	32.0	36.0
12	33.8862064407849	36.0	36.0	36.0	32.0	36.0
13	34.045720420507664	36.0	36.0	36.0	32.0	36.0
14	33.926956057672044	36.0	36.0	36.0	32.0	36.0
15	33.856799957106325	36.0	36.0	36.0	32.0	36.0
16	33.85858553111475	36.0	36.0	36.0	32.0	36.0
17	33.772195295910265	36.0	36.0	36.0	32.0	36.0
18	33.79601437935717	36.0	36.0	36.0	32.0	36.0
19	33.78155147927076	36.0	36.0	36.0	32.0	36.0
20	33.77896913026835	36.0	36.0	36.0	32.0	36.0
21	33.76218635557113	36.0	36.0	36.0	32.0	36.0
22	33.708213889820605	36.0	36.0	36.0	27.0	36.0
23	33.672258015444214	36.0	36.0	36.0	27.0	36.0
24	33.64069500226314	36.0	36.0	36.0	27.0	36.0
25	33.17277361240824	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	66.0
5	154.0
6	274.0
7	82.0
8	228.0
9	265.0
10	182.0
11	60.0
12	124.0
13	84.0
14	231.0
15	287.0
16	477.0
17	687.0
18	951.0
19	1400.0
20	2315.0
21	3435.0
22	5432.0
23	8265.0
24	11636.0
25	16700.0
26	22841.0
27	29142.0
28	39064.0
29	51452.0
30	65360.0
31	88169.0
32	124867.0
33	174691.0
34	365524.0
35	589510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9100587657631	17.31566723302106	10.794631583869812	25.97964241734603
2	17.049209559602872	20.08870788744548	36.63327755318857	26.228804999763078
3	19.319804999460686	23.97223505558039	27.991705182328246	28.716254762630673
4	12.575285663065674	15.080328609954949	36.009477934184474	36.3349077927949
5	14.845414448441547	36.2876890376853	33.267950784091646	15.598945729781514
6	34.08256357125526	35.187852302986336	17.0251889812943	13.704395144464106
7	30.25179519492916	30.480507084079385	20.819332580526424	18.448365140465032
8	28.302548464250926	32.23091865393963	19.81763077251395	19.648902109295488
9	27.327960740214696	14.2622502736428	18.901884235054485	39.50790475108802
10	16.129529597259552	26.340793246753897	31.28987799564814	26.23979916033841
11	37.65039127479369	21.25923258082819	21.98379472570192	19.106581418676203
12	24.817305447312926	23.88613444171566	28.415635881921826	22.88092422904959
13	29.25257667608785	19.41002356959522	25.497608085569617	25.839791668747313
14	23.467257761869863	20.0110736717524	24.921067317187664	31.600601249190074
15	25.30190115966995	27.154377161117978	22.372517444981213	25.17120423423086
16	25.893273981460446	25.839722480894974	23.784231142756568	24.482772394888016
17	23.935244860443124	25.784642657619838	25.359620732381003	24.920491749556035
18	24.994459755979562	24.5675956589457	26.39547042133946	24.04247416373528
19	25.32102944169281	25.156909577383544	25.39325716440328	24.128803816520357
20	25.583610529739026	24.247637250453014	25.24648060956785	24.92227161024011
21	26.65476819199369	24.199787001072487	24.433136939832174	24.712307867101654
22	25.646649821022837	24.529642526446974	24.959524875421533	24.864182777108656
23	24.13769853161522	24.200442013784837	25.65184546998302	26.01001398461692
24	24.559070671051614	25.394607714608913	25.250336487198506	24.79598512714097
25	24.73044375026531	24.711715895953034	25.374744364788636	25.183095988993013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	193.0
2	494.5
3	796.0
4	796.0
5	796.0
6	2283.0
7	3770.0
8	3770.0
9	3770.0
10	3981.5
11	4193.0
12	4193.0
13	4193.0
14	4381.0
15	4569.0
16	4569.0
17	4569.0
18	7394.0
19	10219.0
20	10219.0
21	10219.0
22	16815.5
23	23412.0
24	23412.0
25	23412.0
26	35020.5
27	46629.0
28	46629.0
29	46629.0
30	58193.0
31	69757.0
32	69757.0
33	69757.0
34	85504.5
35	101252.0
36	101252.0
37	101252.0
38	114787.5
39	128323.0
40	128323.0
41	128323.0
42	147015.5
43	165708.0
44	165708.0
45	165708.0
46	190141.5
47	214575.0
48	214575.0
49	214575.0
50	213794.0
51	213013.0
52	213013.0
53	213013.0
54	196437.5
55	179862.0
56	179862.0
57	179862.0
58	165058.0
59	150254.0
60	150254.0
61	150254.0
62	133926.5
63	117599.0
64	117599.0
65	117599.0
66	98549.5
67	79500.0
68	79500.0
69	79500.0
70	60038.5
71	40577.0
72	40577.0
73	40577.0
74	31976.0
75	23375.0
76	23375.0
77	23375.0
78	19227.5
79	15080.0
80	15080.0
81	15080.0
82	10689.5
83	6299.0
84	6299.0
85	6299.0
86	4682.5
87	3066.0
88	3066.0
89	3066.0
90	2060.5
91	1055.0
92	1055.0
93	1055.0
94	612.0
95	169.0
96	169.0
97	169.0
98	445.0
99	721.0
100	721.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008790710027519286
2	0.0031172730594040024
3	0.004551218666729843
4	0.01502525614632729
5	0.03628505841146259
6	0.04769427780888123
7	0.06708371623837413
8	0.08684722743499551
9	0.09426633731637703
10	0.11795761256784745
11	0.11814464895141168
12	0.13167361402922506
13	0.11858106717972826
14	0.12269586761814154
15	0.11134899368191095
16	0.11035146630290167
17	0.12038908555418257
18	0.12787054089675218
19	0.13017732296071113
20	0.11926686725279713
21	0.1296785592712065
22	0.1474470157098093
23	0.13753408738090458
24	0.13229706864110585
25	0.12930448650407803
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1603966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.667024522973875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24247498872722	38.86070924003286
2	13.086418969875574	12.999269837894412
3	3.9037453693122273	5.816622509672284
4	1.5726758374749898	3.1244051754623516
5	0.8218231943834488	2.040875637449574
6	0.48874982669333633	1.45648497767863
7	0.3179113750314615	1.105279844187396
8	0.22555230653928052	0.8962009552079813
9	0.16372597086031648	0.7318603628790341
>10	0.9432922829583986	8.822115787999147
>50	0.10527088575954589	3.676789205131001
>100	0.1083893768994171	11.46511700116117
>500	0.014443432583359088	4.800526268206516
>1k	0.00552618290145913	4.203743197037789
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3541	0.22076527806699145	No Hit
GTACATGGAAGCAGTGGTATCAACG	2622	0.16346979923514587	No Hit
TCCATGTACTCTGCGTTGATACCAC	2257	0.14071370590149668	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2157	0.13447915978268865	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2138	0.13329459602011512	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2112	0.13167361402922506	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2066	0.1288057228145734	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1934	0.1205761219377468	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1923	0.11989032186467793	No Hit
GAACTACGACGGTATCTGATCGTCT	1882	0.11733415795596663	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1846	0.11508972135319577	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1788	0.11147368460428712	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1706	0.10636135678686456	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1695	0.10567555671379568	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1643	0.10243359273201552	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1607	0.10018915612924463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.234546118808005E-5
6	0.0	0.0	0.0	0.0	6.234546118808005E-5
7	0.0	0.0	0.0	0.0	6.234546118808005E-5
8	0.0	0.0	0.0	0.0	6.234546118808005E-5
9	0.0	0.0	0.0	0.0	6.234546118808005E-5
10	0.0	0.0	0.0	0.0	6.234546118808005E-5
11	0.0	0.0	0.0	6.234546118808005E-5	6.234546118808005E-5
12	0.0	0.0	0.0	6.234546118808005E-5	2.493818447523202E-4
13	0.0	0.0	0.0	6.234546118808005E-5	3.740727671284803E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	80	1.2855162E-7	14.252693	15
AGGCCCG	340	0.0	13.692054	10
CGGTTCT	395	0.0	13.469218	12
GCTCGTA	120	5.0931703E-11	13.459195	9
TACGCGT	50	0.0015006225	13.298777	5
TTATACC	50	0.0015027423	13.296285	4
ACGGTAT	355	0.0	12.578271	9
TAGAGTG	235	0.0	12.530761	5
ACGCGCG	130	1.9826984E-10	12.424261	13
CGGTCCA	310	0.0	12.25885	10
GGTTCTA	450	0.0	12.245612	13
GTCTTAG	125	1.4260877E-9	12.151671	1
TCCTATA	80	2.8848131E-5	11.867608	2
TCTCGTC	115	7.066774E-8	11.56596	12
GCGCGCT	140	6.7848305E-10	11.537894	15
TGAGTTA	170	1.0913936E-11	11.177187	12
GCATCGC	290	0.0	11.138993	14
CTCGTAG	145	1.2150849E-9	11.138645	10
GACGGTA	395	0.0	11.064001	8
CGTCGTA	275	0.0	11.055254	10
>>END_MODULE