FastQCFastQC Report
Thu 2 Feb 2017
SRR4062514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603966
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21490.13398039609318402No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19910.12412981322546736No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18860.11758353980071898No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18850.1175211943395309No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18490.11527675773676No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18320.11421688489656263No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17300.10785764785537849No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC16910.10542617486904336No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16170.10081261074112544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT450.001398435624.44437631
TAGGACG3750.021.119944
GTTCGGG1700.020.70582411
ATCGATT708.125057E-418.8570929
GTAATCG600.00741325318.3332814
CGGATCG600.00741325318.3332826
CGCAATA6150.017.8861336
TATACTG1601.1023076E-917.874955
TAGCGGC6300.017.80947530
GACGGAT1651.6607373E-917.33328426
TGTTCGG2055.456968E-1217.17068310
CTAGCGG6550.017.12972329
GTAGGAC5550.017.0449963
TAAACGC3750.017.01328528
GTATCAA45050.016.752321
TAAGAGG8550.016.72514
ACTATAC953.3472397E-416.210483
CGATTTT953.3472397E-416.2104831
TCGGACG1653.1124728E-815.99995423
ATACGAA7050.015.91484840
Produced by FastQC (version 0.11.5)