##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062514_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1603966
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28123414087331	32.0	32.0	32.0	32.0	32.0
2	31.39572596925371	32.0	32.0	32.0	32.0	32.0
3	31.47766162125631	32.0	32.0	32.0	32.0	32.0
4	31.577521593350482	32.0	32.0	32.0	32.0	32.0
5	31.51324279941096	32.0	32.0	32.0	32.0	32.0
6	35.09555003036224	36.0	36.0	36.0	36.0	36.0
7	35.10598666056512	36.0	36.0	36.0	36.0	36.0
8	35.047131921748964	36.0	36.0	36.0	36.0	36.0
9	35.18128563822425	36.0	36.0	36.0	36.0	36.0
10	35.05341260350905	36.0	36.0	36.0	36.0	36.0
11	35.18577949906669	36.0	36.0	36.0	36.0	36.0
12	35.08442510626784	36.0	36.0	36.0	36.0	36.0
13	35.133389984575736	36.0	36.0	36.0	36.0	36.0
14	35.10147596644817	36.0	36.0	36.0	36.0	36.0
15	35.060238184599925	36.0	36.0	36.0	36.0	36.0
16	35.06618344777882	36.0	36.0	36.0	36.0	36.0
17	35.03861303793222	36.0	36.0	36.0	36.0	36.0
18	35.050165651890374	36.0	36.0	36.0	36.0	36.0
19	35.035470203233736	36.0	36.0	36.0	36.0	36.0
20	35.01871174326638	36.0	36.0	36.0	36.0	36.0
21	35.02006276941032	36.0	36.0	36.0	36.0	36.0
22	35.00934184390442	36.0	36.0	36.0	36.0	36.0
23	34.96658033898474	36.0	36.0	36.0	32.0	36.0
24	34.93632907430706	36.0	36.0	36.0	32.0	36.0
25	34.91568836247152	36.0	36.0	36.0	32.0	36.0
26	34.8752966085316	36.0	36.0	36.0	32.0	36.0
27	34.85753438663912	36.0	36.0	36.0	32.0	36.0
28	34.839871294029926	36.0	36.0	36.0	32.0	36.0
29	34.81239939001201	36.0	36.0	36.0	32.0	36.0
30	34.799543755915025	36.0	36.0	36.0	32.0	36.0
31	34.800577443661524	36.0	36.0	36.0	32.0	36.0
32	34.763468801707766	36.0	36.0	36.0	32.0	36.0
33	34.742684071856885	36.0	36.0	36.0	32.0	36.0
34	34.72246419188437	36.0	36.0	36.0	32.0	36.0
35	34.69356021262296	36.0	36.0	36.0	32.0	36.0
36	34.66979287590884	36.0	36.0	36.0	32.0	36.0
37	34.65551514184216	36.0	36.0	36.0	32.0	36.0
38	34.61332409789235	36.0	36.0	36.0	32.0	36.0
39	34.60066173472505	36.0	36.0	36.0	32.0	36.0
40	34.58598997734366	36.0	36.0	36.0	32.0	36.0
41	34.55412084794815	36.0	36.0	36.0	32.0	36.0
42	34.52360461506042	36.0	36.0	36.0	32.0	36.0
43	34.5166138184974	36.0	36.0	36.0	32.0	36.0
44	34.47457240365444	36.0	36.0	36.0	32.0	36.0
45	34.428755971136546	36.0	36.0	36.0	32.0	36.0
46	34.43292750594464	36.0	36.0	36.0	32.0	36.0
47	34.40208395938567	36.0	36.0	36.0	32.0	36.0
48	34.37199105217941	36.0	36.0	36.0	32.0	36.0
49	34.35806993415073	36.0	36.0	36.0	32.0	36.0
50	33.83621036854896	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	37.0
22	118.0
23	397.0
24	1033.0
25	2506.0
26	5267.0
27	9903.0
28	16944.0
29	25946.0
30	37596.0
31	54210.0
32	79658.0
33	129345.0
34	280941.0
35	960056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3159922928709	17.99982540484252	11.356292596541769	26.327889705744806
2	16.420849180123447	20.63912962154748	36.336492300018705	26.60352889831037
3	18.706735903767992	24.33238546918652	28.38561133496721	28.575267292078276
4	12.302380474399083	15.549456783996668	36.1772631090684	35.970899632535854
5	14.42918018784699	36.61463934686854	33.277097692281316	15.679082773003152
6	34.26336425885667	35.5237440311828	16.651215335036202	13.561676374924328
7	30.122895373093943	30.488987921190347	20.90250042706641	18.485616278649296
8	28.327052024202526	32.38817555245907	19.671875633196485	19.612896790141928
9	27.572529592273153	14.001917746386145	18.739736378451912	39.685816282888794
10	16.06012845658823	26.398315176256855	31.23582420076236	26.305732166392552
11	37.926614404544736	21.193840767198306	21.908257406952515	18.971287421304442
12	24.89847041645521	23.796265008111146	28.4065248265861	22.898739748847543
13	29.326743833722162	19.38401437436953	25.51456826391582	25.774673527992487
14	23.591086095341176	19.839572659270832	24.849030465733062	31.72031077965493
15	25.370238521265414	27.06559864735287	22.35683300020075	25.207329831180957
16	26.022621427137484	25.74992237990082	23.705677053004866	24.52177913995683
17	24.052754235438904	25.811706731938205	25.184012628696617	24.95152640392627
18	25.072975362320648	24.39029256231117	26.47500009351819	24.06173198184999
19	25.384328595493923	25.093050600823208	25.420488962983008	24.102131840699865
20	25.638511040757724	24.140162572024593	25.188002738212656	25.03332364900503
21	26.72350910181388	24.25749672998056	24.34976801253892	24.66922615566664
22	25.685519518493532	24.484558899627547	24.97116522420051	24.858756357678406
23	24.19066239558694	24.097206549266005	25.641316586511188	26.070814468635866
24	24.614985604433013	25.277219093172796	25.27004936513617	24.83774593725802
25	24.86312053996157	24.526517395007126	25.30009987742882	25.31026218760248
26	24.29714844329618	25.331272608022864	25.780346964960604	24.591231983720352
27	25.443369747239032	24.819977480819418	24.919667873259158	24.81698489868239
28	24.31497924519597	24.491853318586553	25.842754771609872	25.350412664607603
29	24.34559086663932	24.932822765569842	25.738388469581025	24.983197898209813
30	24.44883495036678	25.024408248055135	26.072123723320818	24.45463307825727
31	24.79298189612498	24.88974205188888	24.90308398058313	25.414192071403008
32	24.612928204213805	24.883320469386508	24.823344135723573	25.680407190676114
33	24.137606408115882	24.409370273434725	25.653785678748804	25.79923763970059
34	24.985816407579712	24.732132726005414	25.843565262605317	24.438485603809557
35	25.760645799225166	24.436490549051538	25.57890877986192	24.223954871861373
36	24.380816052210584	25.13226589591051	25.211132904313434	25.275785147565472
37	25.59056738110409	24.908133962939363	24.92409440100351	24.577204254953035
38	24.450518277818855	24.671719974114165	25.55035455863778	25.327407189429202
39	25.225721742231443	24.58749125604907	24.953583804145474	25.23320319757401
40	25.479530114728117	24.74366663632521	25.374976776315705	24.401826472630965
41	24.163479774508936	25.175720682358605	26.051300339284	24.60949920384846
42	25.784711147243772	25.443806165467347	25.156144207545545	23.615338479743336
43	24.697406304123653	24.138978008262022	25.530341665596403	25.633274022017922
44	24.329630428575168	24.880327887249482	25.323666461757917	25.46637522241743
45	24.426764657106197	25.12160482204735	25.335262717538896	25.116367803307547
46	24.259818636940334	24.687758149336176	25.58921173467002	25.46321147905347
47	24.570159217838782	24.44584236822975	26.019504154078078	24.96449425985339
48	25.222542123710852	25.48701157007069	24.713117360343052	24.57732894587541
49	24.11366575101966	25.26013643680726	25.368679884735712	25.257517927437366
50	24.061919018233553	25.870249119993815	24.88587663329522	25.18195522847741
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	37.0
2	43.0
3	135.5
4	228.0
5	272.5
6	317.0
7	284.5
8	252.0
9	341.5
10	431.0
11	623.0
12	815.0
13	1438.5
14	2062.0
15	2747.5
16	3433.0
17	4020.0
18	4607.0
19	5047.5
20	5488.0
21	6431.5
22	7375.0
23	7896.0
24	8417.0
25	9649.0
26	10881.0
27	13477.0
28	16073.0
29	18221.5
30	20370.0
31	22738.5
32	25107.0
33	29321.0
34	33535.0
35	38780.5
36	44026.0
37	52697.0
38	61368.0
39	65929.5
40	70491.0
41	77279.5
42	84068.0
43	85254.0
44	86440.0
45	96763.0
46	107086.0
47	114549.0
48	122012.0
49	124818.5
50	127625.0
51	119508.0
52	111391.0
53	109337.0
54	107283.0
55	108777.5
56	110272.0
57	108042.5
58	105813.0
59	96456.0
60	87099.0
61	76601.0
62	66103.0
63	57475.0
64	48847.0
65	42431.5
66	36016.0
67	31820.5
68	27625.0
69	25452.5
70	23280.0
71	17904.0
72	12528.0
73	11288.0
74	10048.0
75	7154.5
76	4261.0
77	3932.0
78	3603.0
79	3150.0
80	2697.0
81	2157.0
82	1617.0
83	1510.5
84	1404.0
85	1147.5
86	891.0
87	654.0
88	417.0
89	270.0
90	123.0
91	78.5
92	34.0
93	27.0
94	20.0
95	13.5
96	7.0
97	5.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015960438064148492
2	0.004114800438413283
3	5.611091506927204E-4
4	0.0
5	6.234546118808005E-5
6	3.1172730594040027E-4
7	0.0
8	6.234546118808005E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.234546118808005E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1603966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.830321962320646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65759793856697	41.55506223877262
2	13.106261945217264	13.848160765766782
3	3.82570308797999	6.063393776106815
4	1.4733799138068775	3.1135654087693436
5	0.7653935766184617	2.0217994540322737
6	0.4463455247712026	1.4148346668062162
7	0.2963915726946578	1.0960923548664134
8	0.20974928519082162	0.8864897814398177
9	0.15976899069500577	0.759658249621095
>10	0.85954111890415	8.384903975838728
>50	0.09363827585538792	3.496179898792994
>100	0.0905965993986619	10.259779480850224
>500	0.011842553258043202	4.26947019627842
>1k	0.0037896170425738246	2.8306097520585065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2149	0.13398039609318402	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1991	0.12412981322546736	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1886	0.11758353980071898	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1885	0.1175211943395309	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1849	0.11527675773676	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1832	0.11421688489656263	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1730	0.10785764785537849	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1691	0.10542617486904336	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1617	0.10081261074112544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.493818447523202E-4	0.0
10	0.0	0.0	0.0	2.493818447523202E-4	0.0
11	0.0	0.0	0.0	3.117273059404002E-4	0.0
12	0.0	0.0	0.0	3.117273059404002E-4	0.0
13	0.0	0.0	0.0	3.740727671284803E-4	6.234546118808005E-5
14	0.0	6.234546118808005E-5	0.0	3.740727671284803E-4	6.234546118808005E-5
15	0.0	6.234546118808005E-5	0.0	3.740727671284803E-4	1.8703638356424015E-4
16	0.0	6.234546118808005E-5	0.0	3.740727671284803E-4	1.8703638356424015E-4
17	0.0	6.234546118808005E-5	0.0	4.364182283165603E-4	1.8703638356424015E-4
18	0.0	6.234546118808005E-5	0.0	4.364182283165603E-4	1.8703638356424015E-4
19	0.0	6.234546118808005E-5	0.0	5.611091506927204E-4	1.8703638356424015E-4
20	0.0	6.234546118808005E-5	0.0	6.858000730688806E-4	1.8703638356424015E-4
21	0.0	6.234546118808005E-5	0.0	9.351819178212006E-4	1.8703638356424015E-4
22	0.0	6.234546118808005E-5	0.0	0.0015586365297020012	1.8703638356424015E-4
23	0.0	6.234546118808005E-5	0.0	0.0024314729863351217	2.493818447523202E-4
24	0.0	6.234546118808005E-5	0.0	0.004239491360789443	2.493818447523202E-4
25	0.0	6.234546118808005E-5	0.0	0.0056110915069272045	3.117273059404002E-4
26	0.0	6.234546118808005E-5	0.0	0.007045037114253045	4.987636895046404E-4
27	0.0	6.234546118808005E-5	0.0	0.009476510100588167	4.987636895046404E-4
28	0.0	6.234546118808005E-5	0.0	0.015960438064148492	4.987636895046404E-4
29	0.0	6.234546118808005E-5	0.0	0.0281178029958241	5.611091506927204E-4
30	0.0	6.234546118808005E-5	0.0	0.052557223781551476	5.611091506927204E-4
31	0.0	6.234546118808005E-5	0.0	0.10305704734389631	6.234546118808004E-4
32	0.0	6.234546118808005E-5	0.0	0.1577963622670306	6.234546118808004E-4
33	0.0	6.234546118808005E-5	0.0	0.21852084146422057	6.234546118808004E-4
34	0.0	6.234546118808005E-5	0.0	0.28311073925507146	6.234546118808004E-4
35	0.0	6.234546118808005E-5	0.0	0.3568030743793821	6.858000730688806E-4
36	0.0	6.234546118808005E-5	0.0	0.46877552267317385	8.104909954450406E-4
37	0.0	6.234546118808005E-5	0.0	0.6211478298168415	8.104909954450406E-4
38	0.0	6.234546118808005E-5	0.0	0.8174113416369175	9.351819178212006E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	45	0.0013984356	24.444376	31
TAGGACG	375	0.0	21.11994	4
GTTCGGG	170	0.0	20.705824	11
ATCGATT	70	8.125057E-4	18.85709	29
GTAATCG	60	0.007413253	18.33328	14
CGGATCG	60	0.007413253	18.33328	26
CGCAATA	615	0.0	17.88613	36
TATACTG	160	1.1023076E-9	17.87495	5
TAGCGGC	630	0.0	17.809475	30
GACGGAT	165	1.6607373E-9	17.333284	26
TGTTCGG	205	5.456968E-12	17.170683	10
CTAGCGG	655	0.0	17.129723	29
GTAGGAC	555	0.0	17.044996	3
TAAACGC	375	0.0	17.013285	28
GTATCAA	4505	0.0	16.75232	1
TAAGAGG	855	0.0	16.7251	4
ACTATAC	95	3.3472397E-4	16.21048	3
CGATTTT	95	3.3472397E-4	16.21048	31
TCGGACG	165	3.1124728E-8	15.999954	23
ATACGAA	705	0.0	15.914848	40
>>END_MODULE