FastQCFastQC Report
Thu 2 Feb 2017
SRR4062512_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062512_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785055
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53200.29803003268806844No Hit
GTACATGGAAGCAGTGGTATCAACG44540.24951612135200316No Hit
TCCATGTACTCTGCGTTGATACCAC39140.2192649526205075No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36120.20234670640400435No Hit
TATCAACGCAGAGTACTTTTTTTTT34280.19203890076216137No Hit
GCGCAAGACGGACCAGAGCGAAAGC28400.15909873925453277No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27480.1539448364336113No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23850.13360932856410587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22230.12453397794465718No Hit
GAGTACATGGAAGCAGTGGTATCAA21750.12184498516852423No Hit
CATGTACTCTGCGTTGATACCACTG21090.11814762010134142No Hit
GAATAGGACCGCGGTTCTATTTTGT20960.11741935122447208No Hit
GAACTACGACGGTATCTGATCGTCT19830.11108901406399242No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19010.10649531807143198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA4900.014.3473210
AGAACCG2400.013.0610155
CAAGACG4600.013.0082964
ACGGTAT3150.012.9685999
CGCAAGA4100.012.9675862
AAGACGG4700.012.93475
CGCCAGT2700.012.67233718
CGTTCAA604.085823E-412.66913714
GGTCCAA6100.012.61367311
AGAATTT6050.012.56725517
CCGGTCC5600.012.5539059
AACCGCG1900.012.4999827
GTTATAT1302.0008883E-1012.4133061
GCGTAAC2000.012.34894311
GATATAC4000.012.3402871
CGCATCG2700.012.31583313
TAGAACC2550.012.2916854
TACACCG701.09223285E-412.2128975
CGCGGTC1950.012.17981110
TCGCGTA1950.012.1798119
Produced by FastQC (version 0.11.5)