##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062512_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1785055
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07388455817888	32.0	32.0	32.0	32.0	32.0
2	30.775778337362155	32.0	32.0	32.0	32.0	32.0
3	30.763937245631087	32.0	32.0	32.0	32.0	32.0
4	30.808591331919743	32.0	32.0	32.0	32.0	32.0
5	30.654601678939866	32.0	32.0	32.0	32.0	32.0
6	34.26152751595889	36.0	36.0	36.0	32.0	36.0
7	34.12125956903289	36.0	36.0	36.0	32.0	36.0
8	34.1087445484873	36.0	36.0	36.0	32.0	36.0
9	34.253640924229224	36.0	36.0	36.0	32.0	36.0
10	33.94656467167678	36.0	36.0	36.0	32.0	36.0
11	34.2621952824983	36.0	36.0	36.0	32.0	36.0
12	34.0410065796292	36.0	36.0	36.0	32.0	36.0
13	34.15965614504875	36.0	36.0	36.0	32.0	36.0
14	34.08408816535065	36.0	36.0	36.0	32.0	36.0
15	34.03314127575901	36.0	36.0	36.0	32.0	36.0
16	34.03290374806379	36.0	36.0	36.0	32.0	36.0
17	33.948317558842724	36.0	36.0	36.0	32.0	36.0
18	33.97716036760772	36.0	36.0	36.0	32.0	36.0
19	33.95607922444967	36.0	36.0	36.0	32.0	36.0
20	33.93392360459482	36.0	36.0	36.0	32.0	36.0
21	33.90462646809202	36.0	36.0	36.0	32.0	36.0
22	33.869193386198184	36.0	36.0	36.0	32.0	36.0
23	33.845074241409925	36.0	36.0	36.0	32.0	36.0
24	33.829167728725444	36.0	36.0	36.0	32.0	36.0
25	33.376450585556185	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	53.0
5	171.0
6	310.0
7	79.0
8	276.0
9	319.0
10	222.0
11	72.0
12	144.0
13	119.0
14	345.0
15	420.0
16	702.0
17	930.0
18	1332.0
19	1982.0
20	2990.0
21	4400.0
22	6412.0
23	8935.0
24	12353.0
25	16912.0
26	22440.0
27	28846.0
28	38741.0
29	50436.0
30	65054.0
31	88049.0
32	125028.0
33	179417.0
34	395652.0
35	731909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.251523884650894	17.1431420708696	10.927765626540692	25.67756841793881
2	16.546629183898247	19.97576467872793	37.194159321186916	26.283446816186906
3	19.981434142466345	23.76547411564277	27.700998937253118	28.55209280463777
4	12.570917912831058	14.949994677304588	35.482656417209675	36.99643099265468
5	14.538710398707048	36.540477897024445	33.343682034113726	15.577129670154777
6	34.58635828379168	34.76408609177615	16.84866345437619	13.800892170055976
7	30.261678261308276	30.338365463851062	20.558224534299026	18.841731740541633
8	28.103563318287588	32.87431663349127	19.69744035436934	19.324679693851802
9	27.012317018120847	14.649709387906162	18.9107303573982	39.42724323657479
10	16.11622397171299	26.52467821510061	31.258118940942346	26.100978872244045
11	37.40640070538111	21.317025508366864	22.140138406363686	19.136435379888336
12	24.88840994905873	23.773491129172953	28.629881170690002	22.708217751078315
13	29.484229726819112	19.438224683203504	25.625347758193634	25.452197831783753
14	23.23303656768994	20.15788859621175	25.027485225289098	31.581589610809207
15	24.951892526417748	27.618597331332197	22.789005191849405	24.64050495040065
16	25.487872385651798	25.955343727443175	24.537506232483846	24.019277654421177
17	23.481464363519695	26.23836404470181	25.78924814172476	24.490923450053735
18	24.63605194898445	24.792558586212156	27.312764210129515	23.25862525467388
19	25.0370377849918	25.488671570632082	25.479415630422086	23.99487501395403
20	25.162453759924613	24.576414266202605	25.87340806533488	24.387723908537904
21	26.096580537820067	24.61947698911413	24.691559552538372	24.59238292052743
22	25.122636136912597	25.100754360224542	25.363391787600886	24.41321771526198
23	23.666267231261877	24.86098301501922	25.965994134134	25.506755619584904
24	24.234658959589456	25.653257818337664	25.72904582285241	24.383037399220466
25	24.464577914228126	25.14120760055625	25.618416085185107	24.775798400030517
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	334.0
1	334.0
2	758.5
3	1183.0
4	1183.0
5	1183.0
6	3335.5
7	5488.0
8	5488.0
9	5488.0
10	5614.5
11	5741.0
12	5741.0
13	5741.0
14	5508.5
15	5276.0
16	5276.0
17	5276.0
18	8820.0
19	12364.0
20	12364.0
21	12364.0
22	20025.5
23	27687.0
24	27687.0
25	27687.0
26	41286.0
27	54885.0
28	54885.0
29	54885.0
30	70153.5
31	85422.0
32	85422.0
33	85422.0
34	100739.0
35	116056.0
36	116056.0
37	116056.0
38	129595.0
39	143134.0
40	143134.0
41	143134.0
42	162719.5
43	182305.0
44	182305.0
45	182305.0
46	212238.0
47	242171.0
48	242171.0
49	242171.0
50	238052.0
51	233933.0
52	233933.0
53	233933.0
54	213751.0
55	193569.0
56	193569.0
57	193569.0
58	178996.5
59	164424.0
60	164424.0
61	164424.0
62	146479.0
63	128534.0
64	128534.0
65	128534.0
66	106288.5
67	84043.0
68	84043.0
69	84043.0
70	64154.5
71	44266.0
72	44266.0
73	44266.0
74	34735.0
75	25204.0
76	25204.0
77	25204.0
78	21088.0
79	16972.0
80	16972.0
81	16972.0
82	11867.0
83	6762.0
84	6762.0
85	6762.0
86	5003.0
87	3244.0
88	3244.0
89	3244.0
90	2155.5
91	1067.0
92	1067.0
93	1067.0
94	642.5
95	218.0
96	218.0
97	218.0
98	495.5
99	773.0
100	773.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008010957645562741
2	0.0031931789216578764
3	0.003249199604493979
4	0.013725067294845258
5	0.03428465789569509
6	0.04851391133606528
7	0.06638450916078216
8	0.08991319595194547
9	0.0980361949631804
10	0.12251723336255745
11	0.12262927472822967
12	0.13523392836635287
13	0.12363764701927953
14	0.12767113618347894
15	0.11585077210506119
16	0.11529056527670016
17	0.12430989521331276
18	0.13349728719843365
19	0.13534596973202506
20	0.12548632955287092
21	0.13316116310141704
22	0.1540568777992835
23	0.14223651372086574
24	0.13730669363128867
25	0.134225556075303
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1785055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.45446890386019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40991659125673	36.5394745851374
2	14.674967119077523	14.221354758730254
3	4.611791009891889	6.703856522397256
4	1.977899657211425	3.8335230974122685
5	0.9624019701628869	2.3316338168135693
6	0.5384967953858493	1.56555457361112
7	0.34416804502138637	1.1673535884633783
8	0.23914608279280966	0.9270157145731336
9	0.1662162342848598	0.724852741992523
>10	0.8565536608267976	7.738572918671631
>50	0.09753504364575095	3.3207498611195168
>100	0.09901970673607981	10.350717494616642
>500	0.015518535537613785	5.211016930754743
>1k	0.006253738201724957	5.065791040822335
>5k	1.1580996669861032E-4	0.29853235488424057
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5320	0.29803003268806844	No Hit
GTACATGGAAGCAGTGGTATCAACG	4454	0.24951612135200316	No Hit
TCCATGTACTCTGCGTTGATACCAC	3914	0.2192649526205075	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3612	0.20234670640400435	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3428	0.19203890076216137	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2840	0.15909873925453277	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2748	0.1539448364336113	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2385	0.13360932856410587	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2223	0.12453397794465718	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2175	0.12184498516852423	No Hit
CATGTACTCTGCGTTGATACCACTG	2109	0.11814762010134142	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2096	0.11741935122447208	No Hit
GAACTACGACGGTATCTGATCGTCT	1983	0.11108901406399242	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1901	0.10649531807143198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.6020682836103086E-5
2	0.0	0.0	0.0	0.0	5.6020682836103086E-5
3	0.0	0.0	0.0	0.0	5.6020682836103086E-5
4	0.0	0.0	0.0	0.0	5.6020682836103086E-5
5	0.0	0.0	0.0	0.0	5.6020682836103086E-5
6	0.0	0.0	0.0	0.0	1.6806204850830926E-4
7	0.0	0.0	0.0	1.1204136567220617E-4	1.6806204850830926E-4
8	0.0	0.0	0.0	1.6806204850830926E-4	1.6806204850830926E-4
9	0.0	0.0	0.0	1.6806204850830926E-4	1.6806204850830926E-4
10	0.0	0.0	0.0	1.6806204850830926E-4	1.6806204850830926E-4
11	0.0	0.0	0.0	1.6806204850830926E-4	1.6806204850830926E-4
12	0.0	0.0	0.0	1.6806204850830926E-4	2.2408273134441234E-4
13	0.0	0.0	0.0	1.6806204850830926E-4	2.2408273134441234E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	490	0.0	14.34732	10
AGAACCG	240	0.0	13.061015	5
CAAGACG	460	0.0	13.008296	4
ACGGTAT	315	0.0	12.968599	9
CGCAAGA	410	0.0	12.967586	2
AAGACGG	470	0.0	12.9347	5
CGCCAGT	270	0.0	12.672337	18
CGTTCAA	60	4.085823E-4	12.669137	14
GGTCCAA	610	0.0	12.613673	11
AGAATTT	605	0.0	12.567255	17
CCGGTCC	560	0.0	12.553905	9
AACCGCG	190	0.0	12.499982	7
GTTATAT	130	2.0008883E-10	12.413306	1
GCGTAAC	200	0.0	12.348943	11
GATATAC	400	0.0	12.340287	1
CGCATCG	270	0.0	12.315833	13
TAGAACC	255	0.0	12.291685	4
TACACCG	70	1.09223285E-4	12.212897	5
CGCGGTC	195	0.0	12.179811	10
TCGCGTA	195	0.0	12.179811	9
>>END_MODULE