FastQCFastQC Report
Thu 2 Feb 2017
SRR4062512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785055
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29220.16369243524709323No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24440.13691454885143595No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24120.13512188700068065No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23630.1323768735417116No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20940.11730730985879986No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19410.10873614538487608No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18780.1052068423662016No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18500.10363826324679072No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18250.10223774617588814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG550.004484567319.9998975
GTCCTAC9900.019.1142251
TGTAGGA10200.018.981362
TAGGACG6550.018.8090654
GTAGGAC10700.018.0933653
GGACCGT1355.5302735E-817.9258336
CTAGCGG7250.017.90335829
GTAAACG4600.017.69556227
CGCAATA7600.017.36833436
TAGCGGC7500.017.30657830
TCCTACA10750.017.1915722
CTGTAGG10400.017.1374071
TAGAACG902.2162024E-417.1110254
GATATAC4400.017.0027711
TCTAGCG7700.016.85705828
TAAACGC4700.016.85097928
AGGACGT7050.016.8509775
TAATACT3800.016.7893894
ACGATTA1451.2502642E-716.6895732
GTCCTAT7150.016.6180921
Produced by FastQC (version 0.11.5)