##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1785055
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268302097134264	32.0	32.0	32.0	32.0	32.0
2	31.420003305220288	32.0	32.0	32.0	32.0	32.0
3	31.493887863399166	32.0	32.0	32.0	32.0	32.0
4	31.595455602208336	32.0	32.0	32.0	32.0	32.0
5	31.528356269134566	32.0	32.0	32.0	32.0	32.0
6	35.113737671948485	36.0	36.0	36.0	36.0	36.0
7	35.12170381304778	36.0	36.0	36.0	36.0	36.0
8	35.066688701468586	36.0	36.0	36.0	36.0	36.0
9	35.19051065653439	36.0	36.0	36.0	36.0	36.0
10	35.07134289979861	36.0	36.0	36.0	36.0	36.0
11	35.19348311396568	36.0	36.0	36.0	36.0	36.0
12	35.10404161216321	36.0	36.0	36.0	36.0	36.0
13	35.137734691648156	36.0	36.0	36.0	36.0	36.0
14	35.103615854973654	36.0	36.0	36.0	36.0	36.0
15	35.06997655534423	36.0	36.0	36.0	36.0	36.0
16	35.082557680295565	36.0	36.0	36.0	36.0	36.0
17	35.05610975572181	36.0	36.0	36.0	36.0	36.0
18	35.07497696149419	36.0	36.0	36.0	36.0	36.0
19	35.05146844214884	36.0	36.0	36.0	36.0	36.0
20	35.029958180560264	36.0	36.0	36.0	36.0	36.0
21	35.01983076151715	36.0	36.0	36.0	36.0	36.0
22	35.00657290671716	36.0	36.0	36.0	36.0	36.0
23	34.972520734655234	36.0	36.0	36.0	32.0	36.0
24	34.948980283520676	36.0	36.0	36.0	32.0	36.0
25	34.92615129505813	36.0	36.0	36.0	32.0	36.0
26	34.88993168277728	36.0	36.0	36.0	32.0	36.0
27	34.87360557517835	36.0	36.0	36.0	32.0	36.0
28	34.8539664043965	36.0	36.0	36.0	32.0	36.0
29	34.82657509152379	36.0	36.0	36.0	32.0	36.0
30	34.81074980882942	36.0	36.0	36.0	32.0	36.0
31	34.809311757901014	36.0	36.0	36.0	32.0	36.0
32	34.77006030626507	36.0	36.0	36.0	32.0	36.0
33	34.7398965297988	36.0	36.0	36.0	32.0	36.0
34	34.714411600763	36.0	36.0	36.0	32.0	36.0
35	34.682967191487094	36.0	36.0	36.0	32.0	36.0
36	34.654150712443034	36.0	36.0	36.0	32.0	36.0
37	34.63911588158348	36.0	36.0	36.0	32.0	36.0
38	34.58928044233931	36.0	36.0	36.0	32.0	36.0
39	34.554384038587045	36.0	36.0	36.0	32.0	36.0
40	34.539987283304995	36.0	36.0	36.0	32.0	36.0
41	34.50965264375608	36.0	36.0	36.0	32.0	36.0
42	34.46730100753198	36.0	36.0	36.0	32.0	36.0
43	34.451052768682196	36.0	36.0	36.0	32.0	36.0
44	34.4063247350922	36.0	36.0	36.0	32.0	36.0
45	34.342097582427435	36.0	36.0	36.0	32.0	36.0
46	34.33705291993804	36.0	36.0	36.0	32.0	36.0
47	34.296205439048094	36.0	36.0	36.0	32.0	36.0
48	34.245824358353104	36.0	36.0	36.0	32.0	36.0
49	34.23019178680769	36.0	36.0	36.0	32.0	36.0
50	33.70650428138069	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	6.0
21	31.0
22	130.0
23	399.0
24	1163.0
25	2706.0
26	6025.0
27	11673.0
28	19199.0
29	29913.0
30	43379.0
31	61589.0
32	90314.0
33	144770.0
34	306826.0
35	1066929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.648260985278256	17.788852936644677	11.415444523819529	26.14744155425754
2	15.973848446481192	20.383533709061165	36.94181447411176	26.700803370345888
3	19.302327275049592	24.08970285969741	28.056484723948756	28.551485141304244
4	12.37218859730697	15.308405581462054	35.68977262287037	36.62963319836061
5	14.296758363187688	36.762844842315786	33.17169498979023	15.768701804706298
6	34.630327088693825	35.057317690082805	16.601598497746004	13.710756723477369
7	30.276899186243106	30.30104411407162	20.529575015657787	18.892481684027487
8	28.27319046191854	32.982905288632566	19.45200568049724	19.291898568951655
9	27.204203792040023	14.346336667497638	18.804406586911888	39.645052953550454
10	16.157485343588853	26.571170075992057	31.254050995627587	26.017293584791506
11	37.73396337927963	21.112402699076497	22.004476052558605	19.14915786908527
12	24.89895269333438	23.665825422746078	28.632619162994978	22.802602720924565
13	29.716171210410884	19.337555425463083	25.557867964852626	25.388405399273413
14	23.352165619546735	19.99081260801488	25.04835985445827	31.60866191798012
15	25.116256922055623	27.54363310934397	22.691625748226247	24.64848422037416
16	25.63534456921495	25.86340476904073	24.480422171865854	24.02082848987846
17	23.708121038287334	26.065639434079063	25.693550058681662	24.532689468951936
18	24.79268145799429	24.621034085784473	27.281400292988174	23.304884163233066
19	25.200680091089627	25.331320323463423	25.591368333188612	23.876631252258335
20	25.36196363697477	24.279139858435734	25.939144732235142	24.419751772354353
21	26.274316477643545	24.498404811056243	24.67027626599741	24.557002445302807
22	25.24986625061973	24.942256681166686	25.418096361176545	24.389780707037037
23	23.803020075011695	24.636831918344253	25.964802204974074	25.59534580166998
24	24.358297083283148	25.427843959990028	25.7758444417679	24.438014514958923
25	24.731114727557415	24.733523616919367	25.699152126965274	24.836209528557944
26	23.65159616930571	25.902619247026003	26.42198699759951	24.023797586068778
27	24.86573242841257	25.116761108201146	25.785816123312728	24.231690340073555
28	24.002957892053747	25.02197411284246	26.166084518404194	24.8089834766996
29	23.779155264123514	25.14594788395876	26.26148774127408	24.81340911064365
30	24.020548386464284	25.367453663892707	26.464674757920626	24.147323191722382
31	24.355047883678655	25.217430275257623	25.379049945239785	25.048471895823937
32	24.04962312085622	25.405827831635442	25.427115691113162	25.117433356395182
33	23.731817787127007	24.94494567394282	26.147205548288426	25.176030990641745
34	24.486976591757678	25.317651276851414	26.336555456274457	23.858816675116454
35	25.252387181347352	25.100459089495843	26.013540199041486	23.63361353011532
36	23.793664620978063	25.800045376753094	25.688676259274924	24.717613742993912
37	24.91290184336057	25.54313452526673	25.4225219951206	24.121441636252104
38	24.106652175983374	25.285663466951995	25.733828929640822	24.873855427423806
39	24.793745850968175	24.877496771808154	25.465321796807384	24.86343558041629
40	25.277260364526583	25.133063126906453	25.690132797028664	23.8995437115383
41	23.762517121321192	25.416247678642957	26.643660839581973	24.177574360453878
42	25.381402813918896	26.041774623190882	25.56806372912879	23.008758833761426
43	24.208105632599555	24.654646495486134	26.013652240407158	25.12359563150715
44	23.70795297623883	25.466834355243957	25.91556002476114	24.909652643756075
45	24.020548386464284	25.61657764046486	25.769570125290258	24.5933038477806
46	23.728427111127793	24.979314339686272	26.0594503356483	25.232808213537638
47	24.389500603622857	24.83021531549448	26.27722955315102	24.50305452773164
48	24.717725784359583	25.95197346860461	24.979902580032547	24.350398167003256
49	23.73954864135839	25.714333732013863	25.778365372495525	24.767752254132226
50	23.68420020671632	26.347423468744662	24.981919324614648	24.98645699992437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	22.0
2	33.0
3	112.0
4	191.0
5	234.5
6	278.0
7	275.0
8	272.0
9	389.5
10	507.0
11	785.5
12	1064.0
13	1876.5
14	2689.0
15	3947.5
16	5206.0
17	6168.0
18	7130.0
19	7696.5
20	8263.0
21	9305.0
22	10347.0
23	11203.5
24	12060.0
25	12876.5
26	13693.0
27	17016.5
28	20340.0
29	23811.5
30	27283.0
31	30265.5
32	33248.0
33	38428.0
34	43608.0
35	49978.0
36	56348.0
37	64687.5
38	73027.0
39	76612.0
40	80197.0
41	85220.5
42	90244.0
43	92047.5
44	93851.0
45	105137.0
46	116423.0
47	124771.0
48	133119.0
49	135572.5
50	138026.0
51	128595.5
52	119165.0
53	116525.5
54	113886.0
55	116493.0
56	119100.0
57	115732.5
58	112365.0
59	103603.0
60	94841.0
61	83607.5
62	72374.0
63	62921.0
64	53468.0
65	46238.0
66	39008.0
67	34008.5
68	29009.0
69	26609.5
70	24210.0
71	18686.0
72	13162.0
73	11981.0
74	10800.0
75	7880.0
76	4960.0
77	4429.5
78	3899.0
79	3417.0
80	2935.0
81	2381.0
82	1827.0
83	1538.5
84	1250.0
85	1034.5
86	819.0
87	587.5
88	356.0
89	223.5
90	91.0
91	64.0
92	37.0
93	28.0
94	19.0
95	12.0
96	5.0
97	5.0
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015685791194108865
2	0.004201551212707731
3	3.361240970166185E-4
4	1.6806204850830926E-4
5	0.0
6	2.2408273134441237E-4
7	5.602068283610309E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1204136567220619E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1785055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.230531507253765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29917336625073	39.851463784833754
2	14.307229259505288	14.945483772401879
3	4.4491816946631495	6.971493740538009
4	1.8272306883752378	3.8174892016081534
5	0.946380143763136	2.4714968958329906
6	0.5239657159961635	1.6420204702835037
7	0.330746444504953	1.2092543813439671
8	0.20711151793093746	0.8654035730245585
9	0.1567051606784567	0.7366314448948855
>10	0.7684584877217447	7.350701052535403
>50	0.08238967792445463	3.038129846068779
>100	0.08518707189149832	9.559027011928388
>500	0.011831025081998145	4.139809644657447
>1k	0.004409745712381127	3.401595180048278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2922	0.16369243524709323	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2444	0.13691454885143595	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2412	0.13512188700068065	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2363	0.1323768735417116	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2094	0.11730730985879986	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1941	0.10873614538487608	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1878	0.1052068423662016	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1850	0.10363826324679072	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1825	0.10223774617588814	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.6020682836103086E-5
6	0.0	0.0	0.0	0.0	5.6020682836103086E-5
7	0.0	0.0	0.0	0.0	5.6020682836103086E-5
8	0.0	0.0	0.0	0.0	5.6020682836103086E-5
9	0.0	0.0	0.0	5.6020682836103086E-5	5.6020682836103086E-5
10	0.0	0.0	0.0	5.6020682836103086E-5	5.6020682836103086E-5
11	0.0	0.0	0.0	5.6020682836103086E-5	5.6020682836103086E-5
12	0.0	0.0	0.0	5.6020682836103086E-5	2.2408273134441234E-4
13	0.0	0.0	0.0	1.1204136567220617E-4	2.2408273134441234E-4
14	0.0	0.0	0.0	1.6806204850830926E-4	2.2408273134441234E-4
15	0.0	0.0	0.0	1.6806204850830926E-4	3.361240970166185E-4
16	0.0	0.0	0.0	2.2408273134441234E-4	3.361240970166185E-4
17	0.0	0.0	0.0	2.8010341418051543E-4	3.361240970166185E-4
18	0.0	0.0	0.0	2.8010341418051543E-4	3.361240970166185E-4
19	0.0	0.0	0.0	2.8010341418051543E-4	3.361240970166185E-4
20	0.0	0.0	0.0	3.921447798527216E-4	3.361240970166185E-4
21	0.0	0.0	0.0	5.602068283610309E-4	3.921447798527216E-4
22	0.0	0.0	0.0	8.963309253776494E-4	4.481654626888247E-4
23	0.0	0.0	0.0	0.0020167445820997113	4.481654626888247E-4
24	0.0	0.0	0.0	0.003753385750018907	4.481654626888247E-4
25	0.0	0.0	0.0	0.004481654626888247	4.481654626888247E-4
26	0.0	0.0	0.0	0.005714109649282515	4.481654626888247E-4
27	0.0	0.0	0.0	0.007562792182873917	5.041861455249278E-4
28	0.0	0.0	0.0	0.012828736369467608	5.041861455249278E-4
29	0.0	0.0	0.0	0.022800417914293956	5.602068283610309E-4
30	0.0	0.0	0.0	0.047057373582326596	5.602068283610309E-4
31	0.0	0.0	0.0	0.09221004394822568	5.602068283610309E-4
32	0.0	0.0	0.0	0.146718168347754	6.16227511197134E-4
33	0.0	0.0	0.0	0.20453151303461237	6.16227511197134E-4
34	0.0	0.0	0.0	0.27114010492673896	6.72248194033237E-4
35	0.0	0.0	0.0	0.34878477133757785	6.72248194033237E-4
36	0.0	0.0	0.0	0.4569607098940929	6.72248194033237E-4
37	0.0	0.0	0.0	0.6182442557792337	6.72248194033237E-4
38	0.0	0.0	0.0	0.8307307057765727	6.72248194033237E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	55	0.0044845673	19.999897	5
GTCCTAC	990	0.0	19.114225	1
TGTAGGA	1020	0.0	18.98136	2
TAGGACG	655	0.0	18.809065	4
GTAGGAC	1070	0.0	18.093365	3
GGACCGT	135	5.5302735E-8	17.925833	6
CTAGCGG	725	0.0	17.903358	29
GTAAACG	460	0.0	17.695562	27
CGCAATA	760	0.0	17.368334	36
TAGCGGC	750	0.0	17.306578	30
TCCTACA	1075	0.0	17.191572	2
CTGTAGG	1040	0.0	17.137407	1
TAGAACG	90	2.2162024E-4	17.111025	4
GATATAC	440	0.0	17.002771	1
TCTAGCG	770	0.0	16.857058	28
TAAACGC	470	0.0	16.850979	28
AGGACGT	705	0.0	16.850977	5
TAATACT	380	0.0	16.789389	4
ACGATTA	145	1.2502642E-7	16.68957	32
GTCCTAT	715	0.0	16.618092	1
>>END_MODULE