FastQCFastQC Report
Thu 2 Feb 2017
SRR4062511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749266
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54170.7229742174341289No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39410.5259814271567107No Hit
GTATTAGAGGCACTGCCTGCCCAGT37590.5016909882471645No Hit
GTACATGGAAGCAGTGGTATCAACG36540.4876772734916572No Hit
TATCAACGCAGAGTACTTTTTTTTT33090.44163221072356146No Hit
TCCATGTACTCTGCGTTGATACCAC32350.43175587841968005No Hit
CCATAGGGTCTTCTCGTCTTATTAT31750.423748041416533No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29800.397722571156305No Hit
CTCTAATACTTGTAATGCTAGAGGT29310.3911828376037349No Hit
CCATTGGGATGTCCTGATCCAACAT24830.3313909879802367No Hit
GATATATTTTGATCAACGGACCAAG23410.31243910707278855No Hit
GTCAGGATACCGCGGCCGTTAAACT22730.3033635584692219No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22530.30069427946817284No Hit
CTTCTACACCATTGGGATGTCCTGA22400.29895924811749097No Hit
ATCTATAACTTTATAGATGCAACAC20350.27159913835673843No Hit
GTATCCTGACCGTGCAAAGGTAGCA19450.2595873828520179No Hit
GTTATAGATTAACCCAATTTTAAGT18900.252246865599133No Hit
ATCCTGACCGTGCAAAGGTAGCATA18850.25157954584887077No Hit
GTTATATAATTTAAGCTCCATAGGG18500.24690830759703497No Hit
GTACATGGGGTGGTATCAACGCAAA18320.24450595649609083No Hit
ATCGTAAATAGATAGAAACCGACCT18290.2441055646459335No Hit
GTTCATGCTAGTCCCTAATTAAGGA18250.24357170884572368No Hit
CCTCTAGCATTACAAGTATTAGAGG18190.242770925145409No Hit
CTGTTAGTATGAGTAACAAGAATTC18170.24250399724530408No Hit
GGTCAGGATACCGCGGCCGTTAAAC18140.24210360539514672No Hit
GCCTAAAGGAAAGATCCAAAAAGAT17580.23462962419220942No Hit
GTACATGGGAGAAATCGTAAATAGA17340.23142648939095062No Hit
GGTATCAACGCAGAGTACTTTTTTT17090.22808989063963933No Hit
ACCTATAACTTCTCTGTTAACCCAA16440.21941473388622998No Hit
CTATAGAACTAGTACCGCAAGGGAA16050.2142096398341844No Hit
GAGTACATGGAAGCAGTGGTATCAA15960.21300846428371234No Hit
TGCTAGAGGTGATGTTTTTGGTAAA15900.2122076805833976No Hit
ATAAATAATCCACCTATAACTTCTC15310.204333307530303No Hit
AAATATATCTGGGTCAATAAGATAT15260.20366598778004072No Hit
CACTATAAATAATCCACCTATAACT15140.20206442037941133No Hit
CCTATAACTTCTCTGTTAACCCAAC15120.20179749247930642No Hit
TCCCAATGGTGTAGAAGCTATTAAT14630.1952577589267363No Hit
GTGTAGAAGCTATTAATGGTTCGTT14610.1949908310266314No Hit
GATTAAAGATAAGAGACAGTTGGAC14550.19419004732631667No Hit
CATGTACTCTGCGTTGATACCACTG14170.18911841722432354No Hit
GATTAAACCTTGTACCTTTTGCATA14090.1880507056239039No Hit
GTATCAACGCAGAGTACATGGGAGA13960.18631567427322207No Hit
GACTATAGGCAATAATCACACTATA13800.18418025107238284No Hit
TACTAACAGTGTTGCATCTATAAAG13550.18084365232107155No Hit
GATAGAAACCGACCTGGATTGCTCC13440.1793755488704946No Hit
AGCATGAACGGCTAAACGAGGGTCC12850.17150117581739996No Hit
CCAATAAAGAAAGCGTTCAAGCTCA12810.17096732001719014No Hit
CCTATAGTCTGATTAACTAACAATG12790.17070039211708526No Hit
GTTAGTATGAGTAACAAGAATTCCA12590.16803111311603622No Hit
GACCTGGATTGCTCCGGTCTGAACT12510.16696340151561662No Hit
GTCCTGATCCAACATCGAGGTCGTA12460.16629608176535438No Hit
CTGTTAACCCAACACCGGAATGCCT12450.1661626178153019No Hit
ATCCTAGCCCTAGCCCTACACAAAT12160.16229216326378082No Hit
ATTATAACCTAGACTTACAAGTCAA12070.16109098771330876No Hit
GGATTGCTCCGGTCTGAACTCAGAT12030.16055713191309895No Hit
AGGGATAACAGCGCAATCCTATTTA11850.1581547808121548No Hit
ACCCTATGGAGCTTAAATTATATAA11790.15735399711184012No Hit
CTTTATTGGTGGCTGCTTTTAGGCC11790.15735399711184012No Hit
GTTATACGCGTATGCCTGGAGAATT11730.15655321341152542No Hit
ACCTTTGCACGGTCAGGATACCGCG11700.15615282156136806No Hit
GGATACCGCGGCCGTTAAACTTTAG11630.1552185739110009No Hit
CCATTAATAGCTTCTACACCATTGG11620.15508510996094846No Hit
CTATTAAAGGTTTTTTCCGTTCCAG11420.15241583095989944No Hit
ATTTAAGAGTTCATATCGACAATTA11040.14734420085790628No Hit
CTATTATATAAATCAAAACATTTAT10920.14574263345727687No Hit
CTTAAATAGGATTGCGCTGTTATCC10910.14560916950722438No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10870.1450753137070146No Hit
GTTATAGGTGGATTATTTATAGTGT10610.14160525100565086No Hit
CCTTTAGGCATTCCGGTGTTGGGTT10550.14080446730533616No Hit
GTTCTATAGCTCCTAGATGTACGAA10330.1378682604041822No Hit
GTACATGGGTGGTATCAACGCAAAA10060.13426473375276604No Hit
GGGCAGGCAGTGCCTCTAATACTTG9980.13319702215234644No Hit
CTATAGTATAAGTTTGAAATTTCGG9970.13306355820229399No Hit
CAGTTGGACCCTCGTTTAGCCGTTC9850.13146199080166454No Hit
GTTTAGATTATAGCCAAAAGAGGGA9610.12825885600040574No Hit
GTTTAAAATTGAACTTAAATTCATT9610.12825885600040574No Hit
GCTATCACCAAGCTCGTTAGGCTTT9570.12772500020019592No Hit
CTCTTAAATAGGATTGCGCTGTTAT9450.1261234327995665No Hit
GGTTTAATCTTTGCTTGTTCTTACT9420.12572304094940917No Hit
ACGTAGGACTTTAATCGTTGAACAA9380.12518918514919936No Hit
TTATATAACTTATCTATTTAATTTA9360.12492225724909445No Hit
GTTATCCCTAGGGTAACTTGGTCCG9350.124788793299042No Hit
CAATTAGGGTTTACGACCTCGATGT9240.12332068984846503No Hit
GCTTCCATGTACTCTGCGTTGATAC9110.12158565849778316No Hit
AATCTAAACTTACTTTTTGATTTTG9090.12131873059767827No Hit
CCTTTAATAGTGAATAATTAACAAA8960.11958369924699638No Hit
GCTTTTAACCATTGTAGGCCTAAAA8910.11891637949673413No Hit
GCGGTATCCTGACCGTGCAAAGGTA8880.11851598764657678No Hit
GGTTATAATCATTCGGAGGATTTTT8810.11758173999620962No Hit
TCTTTAATCAGTGAAATTGACCTTT8730.11651402839579002No Hit
GATAAAAGGAACTCGGCAAACAAGA8720.11638056444573756No Hit
GCGTTGATACCACTGCTTCCATGTA8700.11611363654563266No Hit
GGGATGTCCTGATCCAACATCGAGG8700.11611363654563266No Hit
CATGGAAGCAGTGGTATCAACGCAG8670.11571324469547531No Hit
CTATTTAAGAGTTCATATCGACAAT8630.1151793888952655No Hit
CCCTAATTGTCGATATGAACTCTTA8560.11424514124489835No Hit
GGTCTGAACTCAGATCACGTAGGAC8510.11357782149463609No Hit
CATTTATCCTACTAAAAGTATTGGA8500.11344435754458364No Hit
ATTTCAGCCTCTTCACTGAAAGGTC8220.10970736694311499No Hit
GTATAAGTTTGAAATTTCGGTTGGG8210.10957390299306254No Hit
TATCAACGCAGAGTACATGGGAGAA8200.1094404390430101No Hit
CTGCCTGCCCAGTGACTAAAGTTTA8100.10810579954248559No Hit
GCGTATAACAACTCGGATAACCATT8010.10690462399201352No Hit
ATGCAACACTGTTAGTATGAGTAAC8010.10690462399201352No Hit
ACTAAAAGTATTGGAGAAAGAAATT7970.10637076819180372No Hit
AAGTTACCCTAGGGATAACAGCGCA7910.105569984491489No Hit
CGTCTATGTGGCAAAATAGTGAGAA7890.10530305659138411No Hit
TGCTAGTCCCTAATTAAGGAACAAG7780.10383495314080715No Hit
ATTTTAAACTTGCTAAAAAAACAAC7770.10370148919075468No Hit
CGTTTAGCCGTTCATGCTAGTCCCT7680.10250031364028261No Hit
GTGCAAAGGTAGCATAATCACTTGT7670.10236684969023017No Hit
GATTATAACCTAGACTTACAAGTCA7610.10156606598991547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT250.00603804618.995194
CATTTAC250.00604504118.991381
ATGTATG402.7584203E-416.6252337
AGAGTGC350.002168581616.2859426
AAGGTGC350.00217245816.281595
TAAGACC350.00217245816.281594
GTTTAAA1150.015.6885331
TGAACTT1100.015.54879110
ACTTGCT1100.015.5467138
AACTTGC1100.015.5456747
GTCCTAT508.738073E-515.1931051
AATCGTA2450.015.12468313
ATTGAAC1200.015.0428848
AATGTCC704.422982E-714.9297798
CTTGCTA1151.8189894E-1214.8717639
AAGAGTG456.769517E-414.7740365
ACGGACC3750.014.69452316
CGGACCA3750.014.69452317
TGCTAAA1250.014.44309911
CCGGTCG400.00527054814.2521059
Produced by FastQC (version 0.11.5)