##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062511_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 749266 Sequences flagged as poor quality 0 Sequence length 25 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.913883720868157 32.0 32.0 32.0 32.0 32.0 2 30.754295537232437 32.0 32.0 32.0 32.0 32.0 3 30.779564266895868 32.0 32.0 32.0 32.0 32.0 4 30.774441920492855 32.0 32.0 32.0 32.0 32.0 5 30.72587705834777 32.0 32.0 32.0 32.0 32.0 6 34.248916940045326 36.0 36.0 36.0 32.0 36.0 7 34.0038063918555 36.0 36.0 36.0 32.0 36.0 8 33.96949547957601 36.0 36.0 36.0 32.0 36.0 9 34.09901423526491 36.0 36.0 36.0 32.0 36.0 10 33.90596797399054 36.0 36.0 36.0 32.0 36.0 11 34.26456959210748 36.0 36.0 36.0 32.0 36.0 12 34.02643920850539 36.0 36.0 36.0 32.0 36.0 13 34.17491251438074 36.0 36.0 36.0 32.0 36.0 14 34.09112651581681 36.0 36.0 36.0 32.0 36.0 15 34.07590361767383 36.0 36.0 36.0 32.0 36.0 16 34.06060864899782 36.0 36.0 36.0 32.0 36.0 17 34.01718081429025 36.0 36.0 36.0 32.0 36.0 18 34.08857201581281 36.0 36.0 36.0 32.0 36.0 19 34.01776271711248 36.0 36.0 36.0 32.0 36.0 20 33.95222124052072 36.0 36.0 36.0 32.0 36.0 21 33.904498535900466 36.0 36.0 36.0 32.0 36.0 22 33.864897112640904 36.0 36.0 36.0 32.0 36.0 23 33.89152850923437 36.0 36.0 36.0 32.0 36.0 24 33.860237886144574 36.0 36.0 36.0 32.0 36.0 25 33.43478017152787 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 25.0 5 73.0 6 121.0 7 30.0 8 104.0 9 153.0 10 96.0 11 31.0 12 54.0 13 49.0 14 163.0 15 234.0 16 374.0 17 552.0 18 797.0 19 1165.0 20 1698.0 21 2472.0 22 3379.0 23 4373.0 24 5553.0 25 7112.0 26 9045.0 27 11112.0 28 14804.0 29 19482.0 30 25564.0 31 35197.0 32 51289.0 33 74796.0 34 168176.0 35 311188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53758470690776 22.66748175723204 12.387463444389422 23.40747009147078 2 14.187328742897792 22.910446466347853 41.11064693642483 21.791577854329528 3 16.394009324227106 28.62281459256224 32.69178664524025 22.2913894379704 4 9.289992404836877 19.033024943903914 43.40468871177224 28.272293939486975 5 9.578268856941447 41.57992972037682 37.81882169482836 11.022979727853373 6 25.123844672742955 43.74140431179749 20.17793575298896 10.956815262470592 7 24.47938438280022 36.60662653900961 23.25926143564773 15.65472764254244 8 24.24776774979561 35.79441924090134 22.17275024980897 17.785062759494078 9 25.68771183100812 16.50802302637292 23.584664937951967 34.219600204666996 10 14.020925757656745 27.97356887059704 36.272248650382174 21.73325672136405 11 30.121222487092048 25.60277186868633 27.81235635562872 16.4636492885929 12 21.410701541965025 29.996151101346314 31.63567501396562 16.957472342723044 13 24.11300322835824 23.59227011309944 30.365141213948217 21.929585444594103 14 19.728164784898457 25.90225317744658 29.409705193359535 24.95987684429543 15 22.565619638590924 30.280570387117116 27.902309447838334 19.251500526453626 16 21.472282794595518 30.526071062083123 27.910012613305966 20.091633530015393 17 17.97824277175489 29.646194489469487 30.788976800931117 21.586585937844507 18 18.530209261830528 28.901759595454745 33.310704172273844 19.25732697044088 19 20.679807182157266 30.233431470683747 30.643710634248823 18.44305071291016 20 19.06056159986744 30.552326280596827 31.59901224273814 18.78809987679759 21 21.707312835850416 27.751250522860882 29.464229633816974 21.07720700747173 22 19.243971555365448 30.761107843661446 29.42576057317008 20.569160027803026 23 20.588129157461278 30.003622011673304 30.396697153045366 19.01155167782006 24 19.55736423230359 32.023869187231455 29.45893390533849 18.95983267512646 25 20.155317437195468 30.134015080037525 31.305912005495312 18.404755477271696 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 237.0 1 237.0 2 731.0 3 1225.0 4 1225.0 5 1225.0 6 4495.5 7 7766.0 8 7766.0 9 7766.0 10 7594.0 11 7422.0 12 7422.0 13 7422.0 14 7627.5 15 7833.0 16 7833.0 17 7833.0 18 12386.0 19 16939.0 20 16939.0 21 16939.0 22 25875.0 23 34811.0 24 34811.0 25 34811.0 26 50807.0 27 66803.0 28 66803.0 29 66803.0 30 75920.5 31 85038.0 32 85038.0 33 85038.0 34 89379.5 35 93721.0 36 93721.0 37 93721.0 38 87844.0 39 81967.0 40 81967.0 41 81967.0 42 75926.5 43 69886.0 44 69886.0 45 69886.0 46 86176.5 47 102467.0 48 102467.0 49 102467.0 50 86940.0 51 71413.0 52 71413.0 53 71413.0 54 57537.0 55 43661.0 56 43661.0 57 43661.0 58 33326.5 59 22992.0 60 22992.0 61 22992.0 62 18107.5 63 13223.0 64 13223.0 65 13223.0 66 11255.5 67 9288.0 68 9288.0 69 9288.0 70 7387.0 71 5486.0 72 5486.0 73 5486.0 74 4634.5 75 3783.0 76 3783.0 77 3783.0 78 2744.5 79 1706.0 80 1706.0 81 1706.0 82 1254.0 83 802.0 84 802.0 85 802.0 86 562.5 87 323.0 88 323.0 89 323.0 90 202.5 91 82.0 92 82.0 93 82.0 94 60.5 95 39.0 96 39.0 97 39.0 98 196.0 99 353.0 100 353.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008675156753409336 2 0.003603526651416186 3 0.0046712382518357964 4 0.014013714755507391 5 0.03443369911353244 6 0.045911598818043256 7 0.06526387157564871 8 0.09262398133640122 9 0.09782907538844682 10 0.12091833874752089 11 0.11878291554668167 12 0.13346395005245135 13 0.1198506271471013 14 0.1261234327995665 15 0.11504592494521305 16 0.11344435754458364 17 0.12238644219809787 18 0.12932656760082534 19 0.13279663030218908 20 0.12065141084741601 21 0.13066120710134987 22 0.15294968676010923 23 0.14187217890575576 24 0.13626669300355282 25 0.13239623845203172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 749266.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.08144686785436 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37345192212517 18.152308890279944 2 14.293279948264964 7.169922831795517 3 5.099188686412601 3.8368509032226523 4 2.312464820695164 2.3199985413619246 5 1.253538760081786 1.5720282903893676 6 0.7899905256408029 1.1888463236980882 7 0.5577588709543928 0.9792579640841927 8 0.38156606992791064 0.7656183287578314 9 0.313934258889408 0.708653289090053 >10 1.8786938095322454 9.360545719571926 >50 0.26788334098518357 4.7489144376583425 >100 0.3765278768916911 21.23039387073946 >500 0.06390855053324318 11.231519258033861 >1k 0.03727998781105852 16.011557037950634 >5k 5.325712544436932E-4 0.7235843133661909 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5417 0.7229742174341289 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3941 0.5259814271567107 No Hit GTATTAGAGGCACTGCCTGCCCAGT 3759 0.5016909882471645 No Hit GTACATGGAAGCAGTGGTATCAACG 3654 0.4876772734916572 No Hit TATCAACGCAGAGTACTTTTTTTTT 3309 0.44163221072356146 No Hit TCCATGTACTCTGCGTTGATACCAC 3235 0.43175587841968005 No Hit CCATAGGGTCTTCTCGTCTTATTAT 3175 0.423748041416533 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2980 0.397722571156305 No Hit CTCTAATACTTGTAATGCTAGAGGT 2931 0.3911828376037349 No Hit CCATTGGGATGTCCTGATCCAACAT 2483 0.3313909879802367 No Hit GATATATTTTGATCAACGGACCAAG 2341 0.31243910707278855 No Hit GTCAGGATACCGCGGCCGTTAAACT 2273 0.3033635584692219 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2253 0.30069427946817284 No Hit CTTCTACACCATTGGGATGTCCTGA 2240 0.29895924811749097 No Hit ATCTATAACTTTATAGATGCAACAC 2035 0.27159913835673843 No Hit GTATCCTGACCGTGCAAAGGTAGCA 1945 0.2595873828520179 No Hit GTTATAGATTAACCCAATTTTAAGT 1890 0.252246865599133 No Hit ATCCTGACCGTGCAAAGGTAGCATA 1885 0.25157954584887077 No Hit GTTATATAATTTAAGCTCCATAGGG 1850 0.24690830759703497 No Hit GTACATGGGGTGGTATCAACGCAAA 1832 0.24450595649609083 No Hit ATCGTAAATAGATAGAAACCGACCT 1829 0.2441055646459335 No Hit GTTCATGCTAGTCCCTAATTAAGGA 1825 0.24357170884572368 No Hit CCTCTAGCATTACAAGTATTAGAGG 1819 0.242770925145409 No Hit CTGTTAGTATGAGTAACAAGAATTC 1817 0.24250399724530408 No Hit GGTCAGGATACCGCGGCCGTTAAAC 1814 0.24210360539514672 No Hit GCCTAAAGGAAAGATCCAAAAAGAT 1758 0.23462962419220942 No Hit GTACATGGGAGAAATCGTAAATAGA 1734 0.23142648939095062 No Hit GGTATCAACGCAGAGTACTTTTTTT 1709 0.22808989063963933 No Hit ACCTATAACTTCTCTGTTAACCCAA 1644 0.21941473388622998 No Hit CTATAGAACTAGTACCGCAAGGGAA 1605 0.2142096398341844 No Hit GAGTACATGGAAGCAGTGGTATCAA 1596 0.21300846428371234 No Hit TGCTAGAGGTGATGTTTTTGGTAAA 1590 0.2122076805833976 No Hit ATAAATAATCCACCTATAACTTCTC 1531 0.204333307530303 No Hit AAATATATCTGGGTCAATAAGATAT 1526 0.20366598778004072 No Hit CACTATAAATAATCCACCTATAACT 1514 0.20206442037941133 No Hit CCTATAACTTCTCTGTTAACCCAAC 1512 0.20179749247930642 No Hit TCCCAATGGTGTAGAAGCTATTAAT 1463 0.1952577589267363 No Hit GTGTAGAAGCTATTAATGGTTCGTT 1461 0.1949908310266314 No Hit GATTAAAGATAAGAGACAGTTGGAC 1455 0.19419004732631667 No Hit CATGTACTCTGCGTTGATACCACTG 1417 0.18911841722432354 No Hit GATTAAACCTTGTACCTTTTGCATA 1409 0.1880507056239039 No Hit GTATCAACGCAGAGTACATGGGAGA 1396 0.18631567427322207 No Hit GACTATAGGCAATAATCACACTATA 1380 0.18418025107238284 No Hit TACTAACAGTGTTGCATCTATAAAG 1355 0.18084365232107155 No Hit GATAGAAACCGACCTGGATTGCTCC 1344 0.1793755488704946 No Hit AGCATGAACGGCTAAACGAGGGTCC 1285 0.17150117581739996 No Hit CCAATAAAGAAAGCGTTCAAGCTCA 1281 0.17096732001719014 No Hit CCTATAGTCTGATTAACTAACAATG 1279 0.17070039211708526 No Hit GTTAGTATGAGTAACAAGAATTCCA 1259 0.16803111311603622 No Hit GACCTGGATTGCTCCGGTCTGAACT 1251 0.16696340151561662 No Hit GTCCTGATCCAACATCGAGGTCGTA 1246 0.16629608176535438 No Hit CTGTTAACCCAACACCGGAATGCCT 1245 0.1661626178153019 No Hit ATCCTAGCCCTAGCCCTACACAAAT 1216 0.16229216326378082 No Hit ATTATAACCTAGACTTACAAGTCAA 1207 0.16109098771330876 No Hit GGATTGCTCCGGTCTGAACTCAGAT 1203 0.16055713191309895 No Hit AGGGATAACAGCGCAATCCTATTTA 1185 0.1581547808121548 No Hit ACCCTATGGAGCTTAAATTATATAA 1179 0.15735399711184012 No Hit CTTTATTGGTGGCTGCTTTTAGGCC 1179 0.15735399711184012 No Hit GTTATACGCGTATGCCTGGAGAATT 1173 0.15655321341152542 No Hit ACCTTTGCACGGTCAGGATACCGCG 1170 0.15615282156136806 No Hit GGATACCGCGGCCGTTAAACTTTAG 1163 0.1552185739110009 No Hit CCATTAATAGCTTCTACACCATTGG 1162 0.15508510996094846 No Hit CTATTAAAGGTTTTTTCCGTTCCAG 1142 0.15241583095989944 No Hit ATTTAAGAGTTCATATCGACAATTA 1104 0.14734420085790628 No Hit CTATTATATAAATCAAAACATTTAT 1092 0.14574263345727687 No Hit CTTAAATAGGATTGCGCTGTTATCC 1091 0.14560916950722438 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1087 0.1450753137070146 No Hit GTTATAGGTGGATTATTTATAGTGT 1061 0.14160525100565086 No Hit CCTTTAGGCATTCCGGTGTTGGGTT 1055 0.14080446730533616 No Hit GTTCTATAGCTCCTAGATGTACGAA 1033 0.1378682604041822 No Hit GTACATGGGTGGTATCAACGCAAAA 1006 0.13426473375276604 No Hit GGGCAGGCAGTGCCTCTAATACTTG 998 0.13319702215234644 No Hit CTATAGTATAAGTTTGAAATTTCGG 997 0.13306355820229399 No Hit CAGTTGGACCCTCGTTTAGCCGTTC 985 0.13146199080166454 No Hit GTTTAGATTATAGCCAAAAGAGGGA 961 0.12825885600040574 No Hit GTTTAAAATTGAACTTAAATTCATT 961 0.12825885600040574 No Hit GCTATCACCAAGCTCGTTAGGCTTT 957 0.12772500020019592 No Hit CTCTTAAATAGGATTGCGCTGTTAT 945 0.1261234327995665 No Hit GGTTTAATCTTTGCTTGTTCTTACT 942 0.12572304094940917 No Hit ACGTAGGACTTTAATCGTTGAACAA 938 0.12518918514919936 No Hit TTATATAACTTATCTATTTAATTTA 936 0.12492225724909445 No Hit GTTATCCCTAGGGTAACTTGGTCCG 935 0.124788793299042 No Hit CAATTAGGGTTTACGACCTCGATGT 924 0.12332068984846503 No Hit GCTTCCATGTACTCTGCGTTGATAC 911 0.12158565849778316 No Hit AATCTAAACTTACTTTTTGATTTTG 909 0.12131873059767827 No Hit CCTTTAATAGTGAATAATTAACAAA 896 0.11958369924699638 No Hit GCTTTTAACCATTGTAGGCCTAAAA 891 0.11891637949673413 No Hit GCGGTATCCTGACCGTGCAAAGGTA 888 0.11851598764657678 No Hit GGTTATAATCATTCGGAGGATTTTT 881 0.11758173999620962 No Hit TCTTTAATCAGTGAAATTGACCTTT 873 0.11651402839579002 No Hit GATAAAAGGAACTCGGCAAACAAGA 872 0.11638056444573756 No Hit GCGTTGATACCACTGCTTCCATGTA 870 0.11611363654563266 No Hit GGGATGTCCTGATCCAACATCGAGG 870 0.11611363654563266 No Hit CATGGAAGCAGTGGTATCAACGCAG 867 0.11571324469547531 No Hit CTATTTAAGAGTTCATATCGACAAT 863 0.1151793888952655 No Hit CCCTAATTGTCGATATGAACTCTTA 856 0.11424514124489835 No Hit GGTCTGAACTCAGATCACGTAGGAC 851 0.11357782149463609 No Hit CATTTATCCTACTAAAAGTATTGGA 850 0.11344435754458364 No Hit ATTTCAGCCTCTTCACTGAAAGGTC 822 0.10970736694311499 No Hit GTATAAGTTTGAAATTTCGGTTGGG 821 0.10957390299306254 No Hit TATCAACGCAGAGTACATGGGAGAA 820 0.1094404390430101 No Hit CTGCCTGCCCAGTGACTAAAGTTTA 810 0.10810579954248559 No Hit GCGTATAACAACTCGGATAACCATT 801 0.10690462399201352 No Hit ATGCAACACTGTTAGTATGAGTAAC 801 0.10690462399201352 No Hit ACTAAAAGTATTGGAGAAAGAAATT 797 0.10637076819180372 No Hit AAGTTACCCTAGGGATAACAGCGCA 791 0.105569984491489 No Hit CGTCTATGTGGCAAAATAGTGAGAA 789 0.10530305659138411 No Hit TGCTAGTCCCTAATTAAGGAACAAG 778 0.10383495314080715 No Hit ATTTTAAACTTGCTAAAAAAACAAC 777 0.10370148919075468 No Hit CGTTTAGCCGTTCATGCTAGTCCCT 768 0.10250031364028261 No Hit GTGCAAAGGTAGCATAATCACTTGT 767 0.10236684969023017 No Hit GATTATAACCTAGACTTACAAGTCA 761 0.10156606598991547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6692790010490264E-4 0.0 4 0.0 0.0 0.0 2.6692790010490264E-4 0.0 5 0.0 0.0 0.0 2.6692790010490264E-4 0.0 6 0.0 0.0 0.0 2.6692790010490264E-4 0.0 7 0.0 0.0 0.0 4.00391850157354E-4 0.0 8 0.0 0.0 0.0 4.00391850157354E-4 0.0 9 0.0 0.0 0.0 5.338558002098053E-4 0.0 10 0.0 0.0 0.0 5.338558002098053E-4 0.0 11 0.0 0.0 0.0 5.338558002098053E-4 0.0 12 0.0 0.0 0.0 5.338558002098053E-4 0.0 13 0.0 0.0 0.0 5.338558002098053E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGT 25 0.006038046 18.99519 4 CATTTAC 25 0.006045041 18.99138 1 ATGTATG 40 2.7584203E-4 16.625233 7 AGAGTGC 35 0.0021685816 16.285942 6 AAGGTGC 35 0.002172458 16.28159 5 TAAGACC 35 0.002172458 16.28159 4 GTTTAAA 115 0.0 15.688533 1 TGAACTT 110 0.0 15.548791 10 ACTTGCT 110 0.0 15.546713 8 AACTTGC 110 0.0 15.545674 7 GTCCTAT 50 8.738073E-5 15.193105 1 AATCGTA 245 0.0 15.124683 13 ATTGAAC 120 0.0 15.042884 8 AATGTCC 70 4.422982E-7 14.929779 8 CTTGCTA 115 1.8189894E-12 14.871763 9 AAGAGTG 45 6.769517E-4 14.774036 5 ACGGACC 375 0.0 14.694523 16 CGGACCA 375 0.0 14.694523 17 TGCTAAA 125 0.0 14.443099 11 CCGGTCG 40 0.005270548 14.252105 9 >>END_MODULE