FastQCFastQC Report
Thu 2 Feb 2017
SRR4062511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749266
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT35030.46752421703373703No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG31240.41694137996385794No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT26920.359284953541199No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA24120.3219150475265126No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC20920.2792065835097282No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC20630.27533612895820714No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA19830.264659012954011No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC19610.261722806052857No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18690.24944412264803154No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT18410.24570713204656291No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA18190.242770925145409No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC17690.2360977276427864No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA17590.23476308814226188No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT17540.23409576839199964No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC17380.2319603451911604No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA17170.22915760224005893No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT16780.22395250818801332No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG16350.21821355833575792No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT15980.21327539218381725No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC15860.21167382478318783No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT15670.20913800973219127No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA15660.2090045457821388No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG15580.20793683418171915No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC14920.1991282134782574No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC14760.19699279027741817No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC14630.1952577589267363No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG14550.19419004732631667No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA14510.19365619152610689No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT14510.19365619152610689No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT14500.19352272757605443No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC14450.19285540782579216No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT13530.18057672442096667No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT13490.18004286862075683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13390.17870822912023232No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC13300.17750705356976026No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA12730.16989960841677054No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA12680.1692322886665083No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC12410.1656287620150921No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA12140.1620252353636759No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG12030.16055713191309895No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA12010.16029020401299404No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT11650.1554855018111058No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC11610.154951646010896No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA11430.15254929490995187No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA11420.15241583095989944No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC11260.1502804077590602No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC11230.14988001590890285No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT11160.1489457682585357No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT11140.1486788403584308No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG10990.146676881107644No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA10520.1404040754551788No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA10470.13973675570491656No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT10460.1396032917548641No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT10270.1370674767038675No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT10230.1365336209036577No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA10170.135732837203343No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA9940.13266316635213662No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT9840.13132852685161212No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC9430.1258565048994616No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9350.124788793299042No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT9350.124788793299042No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA9290.12398800959872729No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA9280.12385454564867483No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT9180.12251990614815032No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATC9000.12011755504720618No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT8940.1193167713468915No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC8880.11851598764657678No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT8850.11811559579641942No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG8830.11784866789631453No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA8720.11638056444573756No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT8680.11584670864552776No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAA8660.11557978074542286No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA8370.11170932619390175No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT8360.11157586224384933No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT8250.11010775879327235No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGAC8230.10984083089316744No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC8200.1094404390430101No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC8170.10904004719285273No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGTA8060.10757194374227577No Hit
GCGGTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG8000.10677116004196105No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC7990.10663769609190862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.10543652054143654No Hit
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACTTTTAGTAG7900.10543652054143654No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT7850.10476920079117429No Hit
TCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA7780.10383495314080715No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC7780.10383495314080715No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7740.10330109734059732No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG7720.10303416944049243No Hit
TGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTCA7720.10303416944049243No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC7690.10263377759033507No Hit
GGTTATAATCATTCGGAGGATTTTTTATTCTCCGAGGTCACCCCAACCGA7670.10236684969023017No Hit
CTATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGA7640.10196645784007281No Hit
GCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAAT7510.10023142648939096No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCAT7500.1000979625393385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA207.8550196E-443.99966410
TATGTCG404.1222302E-738.49970616
CCGGTTA250.00235231135.19973429
ATGTCGA502.3600096E-630.79976717
GTCCTAG407.018971E-427.5071331
ATACATG1250.026.406851
TGTCGAG651.8123039E-523.6921318
CTGTAGG4200.023.5775451
TGTAGGA4600.022.479592
TTAGAAC500.002579643921.9998343
TCCACCC703.2134387E-521.99983427
GACGTGA1600.021.9998327
AGTAAGT1750.021.37126736
ATCACGG2300.021.04331835
CCGCGGT8550.020.84194642
CGCGGTA8450.020.82824543
AATTCAT2750.020.7998418
CACGGAA2250.020.53317837
GGCCGCG8800.020.49984640
TAGGACG3350.020.3580554
Produced by FastQC (version 0.11.5)