FastQCFastQC Report
Thu 2 Feb 2017
SRR4062510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977672
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG31710.3243419060789304No Hit
TCCATGTACTCTGCGTTGATACCAC27390.2801553077105614No Hit
GTATCAACGCAGAGTACTTTTTTTT25170.2574483057712607No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19110.19546432750452095No Hit
TATCAACGCAGAGTACTTTTTTTTT16070.16437005457863169No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15450.1580284594424306No Hit
GAGTACATGGAAGCAGTGGTATCAA14680.15015260741843892No Hit
CATGTACTCTGCGTTGATACCACTG12370.12652505134646386No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11420.11680809105712345No Hit
GCGCAAGACGGACCAGAGCGAAAGC10820.11067106350596109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT1700.013.9722839
TTACTCT707.273733E-613.568214
GTCGTAG1001.00371835E-813.30161311
CGATAAC500.001497481313.30161310
GTACTAG655.481604E-513.1453421
CGCATCG1550.012.8712113
CCGTCGT1051.9865183E-812.6682039
CGTCGTA1051.9865183E-812.66820310
CGCAAGA2250.012.6597732
GCGCCGA1457.2759576E-1212.45042419
AAGACGG2450.012.4077615
AATCGTA853.938241E-612.2943513
CCGACCA1405.2750693E-1112.2157679
CGACCAT1251.4042598E-912.16147510
GCTCGTT550.00306307912.09299612
ATGCCGG550.00306307912.09299619
TCGAACT550.00306307912.09299619
GATAACG550.003064254512.09237611
CGTGCGC550.003064254512.09237610
ACCGGCG802.8602492E-511.8764418