##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062510_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977672
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011201098118796	32.0	32.0	32.0	32.0	32.0
2	30.61063730985443	32.0	32.0	32.0	32.0	32.0
3	30.59264968210197	32.0	32.0	32.0	32.0	32.0
4	30.666541539493817	32.0	32.0	32.0	32.0	32.0
5	30.42933724193799	32.0	32.0	32.0	32.0	32.0
6	34.04332230032158	36.0	36.0	36.0	32.0	36.0
7	33.9012838661637	36.0	36.0	36.0	32.0	36.0
8	33.8843620355293	36.0	36.0	36.0	32.0	36.0
9	34.090393301639	36.0	36.0	36.0	32.0	36.0
10	33.684863635247815	36.0	36.0	36.0	27.0	36.0
11	34.09562818614014	36.0	36.0	36.0	32.0	36.0
12	33.81091204412114	36.0	36.0	36.0	32.0	36.0
13	33.96123341979723	36.0	36.0	36.0	32.0	36.0
14	33.8445020415845	36.0	36.0	36.0	32.0	36.0
15	33.77935749412891	36.0	36.0	36.0	32.0	36.0
16	33.78275024752678	36.0	36.0	36.0	32.0	36.0
17	33.698334410722616	36.0	36.0	36.0	27.0	36.0
18	33.722501002381165	36.0	36.0	36.0	27.0	36.0
19	33.70338518439722	36.0	36.0	36.0	27.0	36.0
20	33.6977616214845	36.0	36.0	36.0	27.0	36.0
21	33.66545835413104	36.0	36.0	36.0	27.0	36.0
22	33.6195881645378	36.0	36.0	36.0	27.0	36.0
23	33.58871584744168	36.0	36.0	36.0	27.0	36.0
24	33.55245624299356	36.0	36.0	36.0	27.0	36.0
25	33.08365484538782	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	39.0
5	90.0
6	171.0
7	52.0
8	144.0
9	178.0
10	116.0
11	42.0
12	65.0
13	58.0
14	151.0
15	230.0
16	321.0
17	419.0
18	633.0
19	933.0
20	1692.0
21	2412.0
22	3838.0
23	5710.0
24	7882.0
25	10800.0
26	14871.0
27	18817.0
28	25138.0
29	32428.0
30	40816.0
31	54300.0
32	76182.0
33	105950.0
34	218257.0
35	354931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58822175554599	17.376756727451646	11.080207733436716	25.954813783565655
2	16.492999972382442	20.35229772953002	37.15717151218394	25.99753078590359
3	19.5627580224789	24.336250598387117	28.03152119210988	28.069470187024102
4	12.552261876629748	15.32578573116501	35.900929793387206	36.221022598818045
5	14.356416901915726	36.89499894099554	33.60870859778601	15.139875559302714
6	33.89649261940699	35.15659136841567	17.198700401647525	13.748215610529815
7	29.565606330289036	30.685136491995536	21.182947686683036	18.5663094910324
8	27.961511332330748	32.729597556119074	20.06425015381749	19.244640957732695
9	27.375640660738014	14.58853352936238	18.799028591437107	39.23679721846249
10	15.827719758894505	26.803426925904915	32.02399995084495	25.34485336435563
11	37.05664643750294	21.34753675966074	22.499329221958916	19.096487580877398
12	24.719723707902226	23.966413958814286	28.94491569690626	22.36894663637723
13	29.556046891017402	19.71526211062831	25.4766881349996	25.252002863354694
14	23.32535529904315	20.153907444560854	25.25063214912267	31.270105107273327
15	24.916364157676732	27.457644474709564	23.012835938580007	24.613155429033696
16	25.336514584432006	25.816364669680663	24.45617502342418	24.390945722463147
17	23.477700731309916	25.963294202466646	26.090282620918064	24.46872244530537
18	24.439457749883243	24.659045285832505	27.334039345498045	23.567457618786207
19	25.123978623033295	25.580768923511183	25.601150374137887	23.69410207931764
20	25.151099942038247	24.59769832443426	26.031995707143636	24.219206026383862
21	25.937294205966605	24.68268672399967	24.614989128442105	24.76502994159162
22	25.11142593440544	25.30902706285232	25.224311597209205	24.355235405533037
23	23.731785712090826	24.89629148596632	25.956115678269047	25.41580712367381
24	24.194736146716824	25.735668408174465	25.68394552402451	24.385649921084195
25	24.43110705982363	25.08731321118019	25.6951770332968	24.786402695699376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	173.0
1	173.0
2	455.5
3	738.0
4	738.0
5	738.0
6	1919.5
7	3101.0
8	3101.0
9	3101.0
10	3233.5
11	3366.0
12	3366.0
13	3366.0
14	3415.5
15	3465.0
16	3465.0
17	3465.0
18	5504.0
19	7543.0
20	7543.0
21	7543.0
22	11909.5
23	16276.0
24	16276.0
25	16276.0
26	24185.5
27	32095.0
28	32095.0
29	32095.0
30	39565.5
31	47036.0
32	47036.0
33	47036.0
34	55588.5
35	64141.0
36	64141.0
37	64141.0
38	71862.0
39	79583.0
40	79583.0
41	79583.0
42	89956.0
43	100329.0
44	100329.0
45	100329.0
46	116982.0
47	133635.0
48	133635.0
49	133635.0
50	130171.0
51	126707.0
52	126707.0
53	126707.0
54	115957.0
55	105207.0
56	105207.0
57	105207.0
58	96416.0
59	87625.0
60	87625.0
61	87625.0
62	78423.0
63	69221.0
64	69221.0
65	69221.0
66	57677.0
67	46133.0
68	46133.0
69	46133.0
70	35100.0
71	24067.0
72	24067.0
73	24067.0
74	18607.5
75	13148.0
76	13148.0
77	13148.0
78	10653.5
79	8159.0
80	8159.0
81	8159.0
82	5749.5
83	3340.0
84	3340.0
85	3340.0
86	2415.5
87	1491.0
88	1491.0
89	1491.0
90	1007.5
91	524.0
92	524.0
93	524.0
94	321.5
95	119.0
96	119.0
97	119.0
98	284.5
99	450.0
100	450.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007466716853914196
2	0.0033753651531392946
3	0.0045004868708523925
4	0.014626582330270275
5	0.034674205664067294
6	0.04572085525615953
7	0.06699588410018902
8	0.08847548052925726
9	0.09676046772332643
10	0.11967203724766587
11	0.12212684826813083
12	0.13440090337045554
13	0.1222291320606502
14	0.12693418651654131
15	0.11425099624413915
16	0.11425099624413915
17	0.12325196998584392
18	0.13255979510510682
19	0.1323552275200681
20	0.1192629020775884
21	0.13000270029212252
22	0.14964118845584204
23	0.14094706609169536
24	0.1345031871629749
25	0.13317349786022306
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	977672.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.91659679309833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34181911761341	43.31757466988401
2	12.056341614811206	13.000738192912555
3	3.310222816289711	5.354278466436203
4	1.3568986733130273	2.9263743463243417
5	0.6982955016306619	1.8824858501927368
6	0.4403039056630511	1.4243812888836676
7	0.2879793811044341	1.0868807723013643
8	0.20881338619065706	0.9006805718594548
9	0.15150740674370536	0.7351887384511477
>10	0.9253714695918245	9.58616252978745
>50	0.10980250574554094	4.200907569829626
>100	0.10314639496430077	10.929444602593092
>500	0.0075982610708507535	2.7684635157121154
>1k	0.0018995652677126884	1.886438884832283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3171	0.3243419060789304	No Hit
TCCATGTACTCTGCGTTGATACCAC	2739	0.2801553077105614	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2517	0.2574483057712607	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1911	0.19546432750452095	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1607	0.16437005457863169	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1545	0.1580284594424306	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1468	0.15015260741843892	No Hit
CATGTACTCTGCGTTGATACCACTG	1237	0.12652505134646386	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1142	0.11680809105712345	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1082	0.11067106350596109	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0228379251937255E-4
2	0.0	0.0	0.0	0.0	1.0228379251937255E-4
3	0.0	0.0	0.0	0.0	1.0228379251937255E-4
4	0.0	0.0	0.0	0.0	1.0228379251937255E-4
5	0.0	0.0	0.0	0.0	1.0228379251937255E-4
6	0.0	0.0	0.0	0.0	1.0228379251937255E-4
7	0.0	0.0	0.0	0.0	1.0228379251937255E-4
8	0.0	0.0	0.0	0.0	1.0228379251937255E-4
9	0.0	0.0	0.0	1.0228379251937255E-4	1.0228379251937255E-4
10	0.0	0.0	0.0	1.0228379251937255E-4	1.0228379251937255E-4
11	0.0	0.0	0.0	1.0228379251937255E-4	1.0228379251937255E-4
12	0.0	0.0	0.0	1.0228379251937255E-4	2.045675850387451E-4
13	0.0	0.0	0.0	1.0228379251937255E-4	2.045675850387451E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAT	170	0.0	13.972283	9
TTACTCT	70	7.273733E-6	13.56821	4
GTCGTAG	100	1.00371835E-8	13.301613	11
CGATAAC	50	0.0014974813	13.301613	10
GTACTAG	65	5.481604E-5	13.145342	1
CGCATCG	155	0.0	12.87121	13
CCGTCGT	105	1.9865183E-8	12.668203	9
CGTCGTA	105	1.9865183E-8	12.668203	10
CGCAAGA	225	0.0	12.659773	2
GCGCCGA	145	7.2759576E-12	12.450424	19
AAGACGG	245	0.0	12.407761	5
AATCGTA	85	3.938241E-6	12.29435	13
CCGACCA	140	5.2750693E-11	12.215767	9
CGACCAT	125	1.4042598E-9	12.161475	10
GCTCGTT	55	0.003063079	12.092996	12
ATGCCGG	55	0.003063079	12.092996	19
TCGAACT	55	0.003063079	12.092996	19
GATAACG	55	0.0030642545	12.092376	11
CGTGCGC	55	0.0030642545	12.092376	10
ACCGGCG	80	2.8602492E-5	11.876441	8
>>END_MODULE