FastQCFastQC Report
Thu 2 Feb 2017
SRR4062510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977672
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA18880.19311180027657537No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15770.1613015408030505No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9860.10085181942410133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC1750.023.88554836
TTAACGG1850.022.59443735
TAGGACG4800.020.1665274
TCTAATA2050.019.3179262
AGGACGT5000.018.9198675
CGCAATA2250.018.57764836
CACGACT600.007411411518.3332064
GGCCGCG2300.018.17378640
TAATACT2450.017.9590594
TTAGGAC3200.017.8748763
GTCCTAC5450.017.7658861
TGTAGGA5100.017.6870562
GAATCCG1002.4823743E-517.5998789
GGACGTG5150.017.514446
GACGTGA2400.017.4165467
GTCCTAA3300.017.3376461
GCGAGGA2300.017.2172720
TAGAGTG2950.017.1524225
CCCCTAG902.2106206E-417.1153681
CTAATAG902.214944E-417.1109923
Produced by FastQC (version 0.11.5)