FastQCFastQC Report
Thu 2 Feb 2017
SRR4062509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92100
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG73928.026058631921824No Hit
TCCATGTACTCTGCGTTGATACCAC63896.937024972855592No Hit
GAGTACATGGAAGCAGTGGTATCAA31223.3897937024972853No Hit
CATGTACTCTGCGTTGATACCACTG27923.031487513572204No Hit
GCTTCCATGTACTCTGCGTTGATAC20632.239956568946797No Hit
CCCATGTACTCTGCGTTGATACCAC18592.018458197611292No Hit
CATGGAAGCAGTGGTATCAACGCAG17441.8935939196525517No Hit
GCGTTGATACCACTGCTTCCATGTA15961.732899022801303No Hit
GTACATGGGAAGCAGTGGTATCAAC15891.7252985884907708No Hit
GTATCAACGCAGAGTACATGGAAGC12961.4071661237785016No Hit
ACTCTGCGTTGATACCACTGCTTCC11591.2584147665580891No Hit
ACGCAGAGTACATGGAAGCAGTGGT10431.1324647122692726No Hit
CAGTGGTATCAACGCAGAGTACATG9821.0662323561346363No Hit
GTATCAACGCAGAGTACTTTTTTTT9000.9771986970684038No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8930.9695982627578719No Hit
TATCAACGCAGAGTACATGGAAGCA8690.9435396308360479No Hit
TATCAACGCAGAGTACTTTTTTTTT6740.7318132464712269No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6710.7285559174809989No Hit
GTATCAACGCAGAGTACATGGGAAG6100.6623235613463626No Hit
GGTATCAACGCAGAGTACATGGAAG6100.6623235613463626No Hit
AAGCAGTGGTATCAACGCAGAGTAC5800.6297502714440825No Hit
ATACCACTGCTTCCATGTACTCTGC5390.5852334419109664No Hit
GAGTACATGGGAAGCAGTGGTATCA5320.5776330076004343No Hit
GTACATGGGGTGGTATCAACGCAAA5300.5754614549402822No Hit
GCGTTGATACCACTGCTTCCCATGT5130.5570032573289903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4780.5190010857763301No Hit
CCACTGCTTCCATGTACTCTGCGTT4560.495114006514658No Hit
GTACTCTGCGTTGATACCACTGCTT4520.49077090119435396No Hit
GTGGTATCAACGCAGAGTACATGGA4400.47774158523344196No Hit
GCTTCCCATGTACTCTGCGTTGATA4390.47665580890336584No Hit
GAAGCAGTGGTATCAACGCAGAGTA4260.46254071661237783No Hit
ACATGGAAGCAGTGGTATCAACGCA4190.4549402823018458No Hit
CTGCTTCCATGTACTCTGCGTTGAT3920.4256243213897937No Hit
AAAAAGTACTCTGCGTTGATACCAC3800.4125950054288816No Hit
TATCAACGCAGAGTACATGGGAAGC3630.3941368078175896No Hit
TGGAAGCAGTGGTATCAACGCAGAG3600.39087947882736157No Hit
GTTGATACCACTGCTTCCATGTACT3530.38327904451682954No Hit
GGTATCAACGCAGAGTACTTTTTTT3400.3691639522258415No Hit
ACGCAGAGTACATGGGAAGCAGTGG3340.36264929424538545No Hit
GTACATGGGTGGTATCAACGCAAAA3330.36156351791530944No Hit
GATACCACTGCTTCCATGTACTCTG3250.3528773072747014No Hit
GGTATCAACGCAGAGTACATGGGAA3180.3452768729641694No Hit
CCCCATGTACTCTGCGTTGATACCA3090.3355048859934854No Hit
GCAGTGGTATCAACGCAGAGTACAT2860.31053203040173727No Hit
CTTCCATGTACTCTGCGTTGATACC2750.2985884907709012No Hit
GCAGAGTACATGGAAGCAGTGGTAT2670.2899022801302932No Hit
GTACATGGGAGTGGTATCAACGCAA2410.26167209554831705No Hit
CCATGTACTCTGCGTTGATACCACT2370.25732899022801303No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2130.23127035830618892No Hit
TCCATGTACTCTGCGTTGATACCAA2120.2301845819761129No Hit
CTGCTTCCCATGTACTCTGCGTTGA1940.21064060803474483No Hit
GTGGTATCAACGCAGAGTACATGGG1920.20846905537459282No Hit
CACTGCTTCCATGTACTCTGCGTTG1870.20304017372421282No Hit
TGATACCACTGCTTCCATGTACTCT1860.20195439739413681No Hit
ATCAACGCAGAGTACATGGAAGCAG1830.19869706840390877No Hit
GTATCAACGCAGAGTACATGGGGTG1740.18892508143322476No Hit
AAAGTACTCTGCGTTGATACCACTG1720.18675352877307275No Hit
GATACCACTGCTTCCCATGTACTCT1700.18458197611292074No Hit
GTACATGGAAGCAGTGGTATCAACC1600.17372421281216072No Hit
CTGCGTTGATACCACTGCTTCCATG1480.16069489685124863No Hit
CTCTGCGTTGATACCACTGCTTCCA1350.1465798045602606No Hit
TCCATGTACTCTGCGTTGATACAAC1250.13572204125950055No Hit
TATCAACGCAGAGTACATGGGGTGG1220.13246471226927253No Hit
ATACCACTGCTTCCCATGTACTCTG1210.13137893593919653No Hit
GTATCAACGCAGAGTACATGGAATC1190.12920738327904452No Hit
GAGTACATGGGGTGGTATCAACGCA1140.1237785016286645No Hit
CATGGGAAGCAGTGGTATCAACGCA1130.12269272529858849No Hit
TACCACTGCTTCCATGTACTCTGCG1080.11726384364820848No Hit
GTATCAACGCAGAGTACATGGGAGT1080.11726384364820848No Hit
GCAGAGTACATGGGAAGCAGTGGTA1060.11509229098805646No Hit
GTACATGGAAGCAGTGGTATCAAAG1040.11292073832790445No Hit
ATCAACGCAGAGTACTTTTTTTTTT1040.11292073832790445No Hit
AGTGGTATCAACGCAGAGTACATGG1010.10966340933767645No Hit
GTATCAACGCAGAGTACATGGGCAG990.10749185667752444No Hit
TCCATGTACTCTGCGTTGATACAAA950.1031487513572204No Hit
TACCACTGCTTCCCATGTACTCTGC940.1020629750271444No Hit
ACGCAGAGTACATGGGGTGGTATCA930.10097719869706841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCAA350.002149812216.28524817
ATATGGC400.00523031414.24959315
AAAAAGT551.9292734E-413.8177861
AAAAGTA655.3460324E-513.153472
TGGGTGG1201.21001E-711.0890436
CATGGGT1701.2732926E-1010.6173434
ATGGGTG1451.3622412E-810.4824595
GGGAAGC3600.010.2913727
TGGGGTG1752.3719622E-99.7764626
GGGGTGG1752.39379E-99.7711497
AAAGTAC900.00109298599.4997283
ATGGGAG1002.692905E-49.4997275
AGTACTC1002.692905E-49.4997275
TCCATGT14200.09.299031
ACATGGA16850.09.2460273
TACATGG27900.09.1592362
ATGGGGT2106.1118044E-109.0473595
TGATACC19850.09.04508117
AAGTACT950.00179023538.99974254
GATACCA19900.08.97461818
Produced by FastQC (version 0.11.5)