##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062509_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92100
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.651205211726385	32.0	32.0	32.0	32.0	32.0
2	30.813355048859936	32.0	32.0	32.0	32.0	32.0
3	30.8571444082519	32.0	32.0	32.0	32.0	32.0
4	30.685407166123778	32.0	32.0	32.0	32.0	32.0
5	30.84685124864278	32.0	32.0	32.0	32.0	32.0
6	34.30960912052117	36.0	36.0	36.0	32.0	36.0
7	33.99219326818675	36.0	36.0	36.0	32.0	36.0
8	34.01042345276873	36.0	36.0	36.0	32.0	36.0
9	33.89833876221498	36.0	36.0	36.0	32.0	36.0
10	34.03377850162867	36.0	36.0	36.0	32.0	36.0
11	34.18499457111835	36.0	36.0	36.0	32.0	36.0
12	34.105070575461454	36.0	36.0	36.0	32.0	36.0
13	34.02170466883822	36.0	36.0	36.0	32.0	36.0
14	33.95264929424538	36.0	36.0	36.0	32.0	36.0
15	34.053344191096635	36.0	36.0	36.0	32.0	36.0
16	33.89301845819761	36.0	36.0	36.0	32.0	36.0
17	33.781704668838216	36.0	36.0	36.0	32.0	36.0
18	33.782062975027145	36.0	36.0	36.0	32.0	36.0
19	33.74870792616721	36.0	36.0	36.0	32.0	36.0
20	33.722356134636264	36.0	36.0	36.0	27.0	36.0
21	33.35619978284473	36.0	36.0	36.0	21.0	36.0
22	33.27852334419109	36.0	36.0	36.0	21.0	36.0
23	32.97792616720955	36.0	36.0	36.0	14.0	36.0
24	32.96634093376765	36.0	36.0	36.0	14.0	36.0
25	32.03638436482085	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	2.0
5	10.0
6	18.0
7	7.0
8	29.0
9	19.0
10	14.0
11	2.0
12	7.0
13	2.0
14	35.0
15	55.0
16	63.0
17	83.0
18	135.0
19	189.0
20	255.0
21	346.0
22	526.0
23	677.0
24	808.0
25	957.0
26	1149.0
27	1509.0
28	1980.0
29	2657.0
30	3529.0
31	5030.0
32	7369.0
33	10024.0
34	20067.0
35	34546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.878382487078134	14.295487121574077	15.294488120575078	20.531642270772704
2	8.05898193189715	25.280142460041695	36.08408617095205	30.576789437109102
3	21.867636679515716	32.78896791356751	25.244584396547044	20.098811010369726
4	12.883189088816255	20.19350845377841	29.70387342679365	37.21942903061169
5	10.414268025112419	40.45575999826212	33.959333521604066	15.170638455021399
6	32.023424089002845	27.0267921166424	22.68095000108646	18.268833793268293
7	24.401191097200485	33.170318205529476	24.503347243957574	17.92514345331247
8	33.19889989238078	27.310280353512844	25.308997619332324	14.181822134774055
9	27.816974137649886	19.808233772163458	16.632785067455185	35.74200702273148
10	16.41748850156031	30.08839935195555	33.51672846285162	19.977383683632528
11	32.80488865692415	24.733602992345162	14.668145441892833	27.793362908837853
12	18.89802309649638	26.948087253430764	30.626780626780626	23.52710902329223
13	37.15829763173347	22.223430398190636	22.45286301459235	18.165408955483546
14	29.3387845640284	17.289897463220502	23.27682755771092	30.094490415040177
15	30.03533568904594	19.573797227507477	35.43462897526502	14.956238108181571
16	27.64761521651681	23.680405309907695	30.298220245925698	18.37375922764979
17	24.045884527563334	23.24127432858541	34.268783298901816	18.44405784494944
18	29.87592026708135	17.064497536892244	36.203877899453005	16.8557042965734
19	19.24185252443971	40.71073607290047	24.899685736344754	15.147725666315068
20	19.4419373430041	18.50240862974522	44.392731701482155	17.66292232576853
21	19.990213136146153	32.32057416267943	20.14680295780774	27.54240974336668
22	17.390500429623344	32.62962117009822	24.868121947770852	25.11175645250759
23	16.518400904819906	29.99717243779363	23.020706455542022	30.463720201844442
24	15.073245532946897	38.01372470119955	24.244434535785363	22.66859523006819
25	27.67368775894129	24.482117419340806	19.43650025554311	28.40769456617479
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	54.0
2	122.5
3	191.0
4	191.0
5	191.0
6	621.5
7	1052.0
8	1052.0
9	1052.0
10	971.0
11	890.0
12	890.0
13	890.0
14	551.0
15	212.0
16	212.0
17	212.0
18	390.0
19	568.0
20	568.0
21	568.0
22	814.5
23	1061.0
24	1061.0
25	1061.0
26	1504.5
27	1948.0
28	1948.0
29	1948.0
30	2191.5
31	2435.0
32	2435.0
33	2435.0
34	2548.5
35	2662.0
36	2662.0
37	2662.0
38	3221.5
39	3781.0
40	3781.0
41	3781.0
42	7631.0
43	11481.0
44	11481.0
45	11481.0
46	26054.5
47	40628.0
48	40628.0
49	40628.0
50	28199.0
51	15770.0
52	15770.0
53	15770.0
54	9737.0
55	3704.0
56	3704.0
57	3704.0
58	2884.0
59	2064.0
60	2064.0
61	2064.0
62	1782.0
63	1500.0
64	1500.0
65	1500.0
66	1244.5
67	989.0
68	989.0
69	989.0
70	763.5
71	538.0
72	538.0
73	538.0
74	420.0
75	302.0
76	302.0
77	302.0
78	224.5
79	147.0
80	147.0
81	147.0
82	94.0
83	41.0
84	41.0
85	41.0
86	27.0
87	13.0
88	13.0
89	13.0
90	9.0
91	5.0
92	5.0
93	5.0
94	5.5
95	6.0
96	6.0
97	6.0
98	32.0
99	58.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008686210640608035
2	0.004343105320304018
3	0.005428881650380022
4	0.011943539630836048
5	0.03691639522258414
6	0.06297502714440825
7	0.09120521172638436
8	0.11834961997828448
9	0.12269272529858849
10	0.14223669923995658
11	0.14332247557003258
12	0.14983713355048858
13	0.1454940282301846
14	0.1444082519001086
15	0.13572204125950055
16	0.13137893593919653
17	0.14115092290988057
18	0.15526601520086863
19	0.15092290988056462
20	0.15092290988056462
21	0.15200868621064062
22	0.1726384364820847
23	0.16069489685124863
24	0.16178067318132466
25	0.15092290988056462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	92100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.22909880564604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81178579430876	19.337676438653638
2	11.059825229666144	5.359391965255157
3	3.1369034281873183	2.2801302931596092
4	1.541563970423482	1.494028230184582
5	0.8290387631637912	1.004343105320304
6	0.5780864889088058	0.8403908794788273
7	0.4346851893345284	0.7372421281216069
8	0.33609679587721264	0.6514657980456027
9	0.2106206587497199	0.4592833876221498
>10	1.5370826798117858	7.1368078175895775
>50	0.19717678691463142	3.3257328990228014
>100	0.2151019493614161	12.914223669923997
>500	0.0582567779520502	9.666666666666666
>1k	0.04481290611696168	19.829533116178066
>5k	0.008962581223392337	14.963083604777417
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7392	8.026058631921824	No Hit
TCCATGTACTCTGCGTTGATACCAC	6389	6.937024972855592	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3122	3.3897937024972853	No Hit
CATGTACTCTGCGTTGATACCACTG	2792	3.031487513572204	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2063	2.239956568946797	No Hit
CCCATGTACTCTGCGTTGATACCAC	1859	2.018458197611292	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1744	1.8935939196525517	No Hit
GCGTTGATACCACTGCTTCCATGTA	1596	1.732899022801303	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1589	1.7252985884907708	No Hit
GTATCAACGCAGAGTACATGGAAGC	1296	1.4071661237785016	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1159	1.2584147665580891	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1043	1.1324647122692726	No Hit
CAGTGGTATCAACGCAGAGTACATG	982	1.0662323561346363	No Hit
GTATCAACGCAGAGTACTTTTTTTT	900	0.9771986970684038	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	893	0.9695982627578719	No Hit
TATCAACGCAGAGTACATGGAAGCA	869	0.9435396308360479	No Hit
TATCAACGCAGAGTACTTTTTTTTT	674	0.7318132464712269	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	671	0.7285559174809989	No Hit
GTATCAACGCAGAGTACATGGGAAG	610	0.6623235613463626	No Hit
GGTATCAACGCAGAGTACATGGAAG	610	0.6623235613463626	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	580	0.6297502714440825	No Hit
ATACCACTGCTTCCATGTACTCTGC	539	0.5852334419109664	No Hit
GAGTACATGGGAAGCAGTGGTATCA	532	0.5776330076004343	No Hit
GTACATGGGGTGGTATCAACGCAAA	530	0.5754614549402822	No Hit
GCGTTGATACCACTGCTTCCCATGT	513	0.5570032573289903	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	478	0.5190010857763301	No Hit
CCACTGCTTCCATGTACTCTGCGTT	456	0.495114006514658	No Hit
GTACTCTGCGTTGATACCACTGCTT	452	0.49077090119435396	No Hit
GTGGTATCAACGCAGAGTACATGGA	440	0.47774158523344196	No Hit
GCTTCCCATGTACTCTGCGTTGATA	439	0.47665580890336584	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	426	0.46254071661237783	No Hit
ACATGGAAGCAGTGGTATCAACGCA	419	0.4549402823018458	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	392	0.4256243213897937	No Hit
AAAAAGTACTCTGCGTTGATACCAC	380	0.4125950054288816	No Hit
TATCAACGCAGAGTACATGGGAAGC	363	0.3941368078175896	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	360	0.39087947882736157	No Hit
GTTGATACCACTGCTTCCATGTACT	353	0.38327904451682954	No Hit
GGTATCAACGCAGAGTACTTTTTTT	340	0.3691639522258415	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	334	0.36264929424538545	No Hit
GTACATGGGTGGTATCAACGCAAAA	333	0.36156351791530944	No Hit
GATACCACTGCTTCCATGTACTCTG	325	0.3528773072747014	No Hit
GGTATCAACGCAGAGTACATGGGAA	318	0.3452768729641694	No Hit
CCCCATGTACTCTGCGTTGATACCA	309	0.3355048859934854	No Hit
GCAGTGGTATCAACGCAGAGTACAT	286	0.31053203040173727	No Hit
CTTCCATGTACTCTGCGTTGATACC	275	0.2985884907709012	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	267	0.2899022801302932	No Hit
GTACATGGGAGTGGTATCAACGCAA	241	0.26167209554831705	No Hit
CCATGTACTCTGCGTTGATACCACT	237	0.25732899022801303	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	213	0.23127035830618892	No Hit
TCCATGTACTCTGCGTTGATACCAA	212	0.2301845819761129	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	194	0.21064060803474483	No Hit
GTGGTATCAACGCAGAGTACATGGG	192	0.20846905537459282	No Hit
CACTGCTTCCATGTACTCTGCGTTG	187	0.20304017372421282	No Hit
TGATACCACTGCTTCCATGTACTCT	186	0.20195439739413681	No Hit
ATCAACGCAGAGTACATGGAAGCAG	183	0.19869706840390877	No Hit
GTATCAACGCAGAGTACATGGGGTG	174	0.18892508143322476	No Hit
AAAGTACTCTGCGTTGATACCACTG	172	0.18675352877307275	No Hit
GATACCACTGCTTCCCATGTACTCT	170	0.18458197611292074	No Hit
GTACATGGAAGCAGTGGTATCAACC	160	0.17372421281216072	No Hit
CTGCGTTGATACCACTGCTTCCATG	148	0.16069489685124863	No Hit
CTCTGCGTTGATACCACTGCTTCCA	135	0.1465798045602606	No Hit
TCCATGTACTCTGCGTTGATACAAC	125	0.13572204125950055	No Hit
TATCAACGCAGAGTACATGGGGTGG	122	0.13246471226927253	No Hit
ATACCACTGCTTCCCATGTACTCTG	121	0.13137893593919653	No Hit
GTATCAACGCAGAGTACATGGAATC	119	0.12920738327904452	No Hit
GAGTACATGGGGTGGTATCAACGCA	114	0.1237785016286645	No Hit
CATGGGAAGCAGTGGTATCAACGCA	113	0.12269272529858849	No Hit
TACCACTGCTTCCATGTACTCTGCG	108	0.11726384364820848	No Hit
GTATCAACGCAGAGTACATGGGAGT	108	0.11726384364820848	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	106	0.11509229098805646	No Hit
GTACATGGAAGCAGTGGTATCAAAG	104	0.11292073832790445	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	104	0.11292073832790445	No Hit
AGTGGTATCAACGCAGAGTACATGG	101	0.10966340933767645	No Hit
GTATCAACGCAGAGTACATGGGCAG	99	0.10749185667752444	No Hit
TCCATGTACTCTGCGTTGATACAAA	95	0.1031487513572204	No Hit
TACCACTGCTTCCCATGTACTCTGC	94	0.1020629750271444	No Hit
ACGCAGAGTACATGGGGTGGTATCA	93	0.10097719869706841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0010857763300760044	0.0
10	0.0	0.0	0.0	0.0010857763300760044	0.0
11	0.0	0.0	0.0	0.0010857763300760044	0.0
12	0.0	0.0	0.0	0.0010857763300760044	0.0
13	0.0	0.0	0.0	0.0010857763300760044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGCAA	35	0.0021498122	16.285248	17
ATATGGC	40	0.005230314	14.249593	15
AAAAAGT	55	1.9292734E-4	13.817786	1
AAAAGTA	65	5.3460324E-5	13.15347	2
TGGGTGG	120	1.21001E-7	11.089043	6
CATGGGT	170	1.2732926E-10	10.617343	4
ATGGGTG	145	1.3622412E-8	10.482459	5
GGGAAGC	360	0.0	10.291372	7
TGGGGTG	175	2.3719622E-9	9.776462	6
GGGGTGG	175	2.39379E-9	9.771149	7
AAAGTAC	90	0.0010929859	9.499728	3
ATGGGAG	100	2.692905E-4	9.499727	5
AGTACTC	100	2.692905E-4	9.499727	5
TCCATGT	1420	0.0	9.29903	1
ACATGGA	1685	0.0	9.246027	3
TACATGG	2790	0.0	9.159236	2
ATGGGGT	210	6.1118044E-10	9.047359	5
TGATACC	1985	0.0	9.045081	17
AAGTACT	95	0.0017902353	8.9997425	4
GATACCA	1990	0.0	8.974618	18
>>END_MODULE