##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92100
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.640781758957655	32.0	32.0	32.0	32.0	32.0
2	31.436112920738328	32.0	32.0	32.0	32.0	32.0
3	31.505200868621063	32.0	32.0	32.0	32.0	32.0
4	31.645222584147664	32.0	32.0	32.0	32.0	32.0
5	31.585103148751358	32.0	32.0	32.0	32.0	32.0
6	35.151976112920735	36.0	36.0	36.0	36.0	36.0
7	35.173507057546146	36.0	36.0	36.0	36.0	36.0
8	35.207687296416935	36.0	36.0	36.0	36.0	36.0
9	35.18742671009772	36.0	36.0	36.0	36.0	36.0
10	35.142323561346366	36.0	36.0	36.0	36.0	36.0
11	35.186091205211724	36.0	36.0	36.0	36.0	36.0
12	35.05393051031488	36.0	36.0	36.0	36.0	36.0
13	35.07124864277959	36.0	36.0	36.0	36.0	36.0
14	34.99479913137893	36.0	36.0	36.0	36.0	36.0
15	35.00592833876222	36.0	36.0	36.0	36.0	36.0
16	34.873735070575464	36.0	36.0	36.0	36.0	36.0
17	34.86868621064061	36.0	36.0	36.0	32.0	36.0
18	34.84815418023887	36.0	36.0	36.0	32.0	36.0
19	34.71627578718784	36.0	36.0	36.0	32.0	36.0
20	34.6756894679696	36.0	36.0	36.0	32.0	36.0
21	34.39956568946797	36.0	36.0	36.0	32.0	36.0
22	34.327915309446254	36.0	36.0	36.0	32.0	36.0
23	34.02100977198697	36.0	36.0	36.0	32.0	36.0
24	33.941216069489684	36.0	36.0	36.0	32.0	36.0
25	33.88752442996743	36.0	36.0	36.0	32.0	36.0
26	33.68482084690554	36.0	36.0	36.0	27.0	36.0
27	33.48676438653637	36.0	36.0	36.0	21.0	36.0
28	33.33033659066233	36.0	36.0	36.0	21.0	36.0
29	33.12027144408252	36.0	36.0	36.0	14.0	36.0
30	33.02020629750272	36.0	36.0	36.0	14.0	36.0
31	32.92549402823018	36.0	36.0	36.0	14.0	36.0
32	32.78	36.0	36.0	36.0	14.0	36.0
33	32.54690553745928	36.0	36.0	36.0	14.0	36.0
34	32.40641693811075	36.0	36.0	36.0	14.0	36.0
35	31.957480998914225	36.0	32.0	36.0	14.0	36.0
36	31.882681867535286	36.0	32.0	36.0	14.0	36.0
37	31.420542888165038	36.0	32.0	36.0	14.0	36.0
38	31.06986970684039	36.0	32.0	36.0	14.0	36.0
39	30.147937024972855	36.0	21.0	36.0	14.0	36.0
40	29.53343105320304	36.0	14.0	36.0	14.0	36.0
41	29.764343105320304	36.0	14.0	36.0	14.0	36.0
42	28.667090119435397	36.0	14.0	36.0	14.0	36.0
43	29.067111834962	36.0	14.0	36.0	14.0	36.0
44	29.147252985884908	36.0	14.0	36.0	14.0	36.0
45	27.930445168295332	36.0	14.0	36.0	14.0	36.0
46	28.88053203040174	36.0	14.0	36.0	14.0	36.0
47	28.210065146579804	36.0	14.0	36.0	14.0	36.0
48	28.601813246471227	36.0	14.0	36.0	14.0	36.0
49	28.951444082519	36.0	14.0	36.0	14.0	36.0
50	27.867741585233443	36.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	5.0
22	29.0
23	105.0
24	319.0
25	864.0
26	1834.0
27	3460.0
28	5291.0
29	6724.0
30	8069.0
31	9414.0
32	12603.0
33	8744.0
34	10039.0
35	24599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.196172976260286	15.126734866749203	16.10521057318477	20.571881583805737
2	8.313789359391965	26.109663409337674	34.700325732899024	30.876221498371336
3	21.686446106906697	32.22510559289461	26.285844580288604	19.8026037199101
4	13.07057546145494	20.181324647122693	29.676438653637348	37.071661237785015
5	10.51357220412595	40.45276872964169	34.044516829533116	14.98914223669924
6	32.357220412595005	27.355048859934854	21.849077090119433	18.438653637350704
7	23.7828447339848	32.91965255157437	24.5114006514658	18.786102062975026
8	33.06297502714441	27.599348534201955	25.560260586319217	13.777415852334418
9	27.87079261672096	18.92616720955483	16.6199782844734	36.583061889250814
10	16.438653637350704	29.901194353963085	33.89359391965255	19.76655808903366
11	33.3800217155266	24.18675352877307	14.707926167209553	27.72529858849077
12	18.49837133550489	27.44191096634093	30.58197611292074	23.477741585233442
13	38.25190010857764	21.514657980456025	22.561346362649296	17.672095548317046
14	29.09989142236699	16.76872964169381	23.807817589576548	30.32356134636265
15	30.28338762214984	19.934853420195438	35.00651465798046	14.775244299674267
16	28.043431053203037	23.004343105320306	30.46254071661238	18.489685124864277
17	23.838219326818674	23.416938110749186	34.46254071661238	18.282301845819763
18	30.092290988056458	16.288816503800216	36.68946796959826	16.92942453854506
19	19.59826275787188	39.928338762214985	25.585233441910965	14.888165038002171
20	19.75352877307275	18.0586319218241	44.50814332247557	17.679695982627578
21	20.308360477741587	31.5157437567861	20.758957654723126	27.416938110749182
22	17.44299674267101	31.71444082519001	25.347448425624318	25.495114006514658
23	16.596091205211728	28.180238870792618	23.874049945711185	31.349619978284473
24	15.546145494028229	36.868621064060804	24.675352877307276	22.90988056460369
25	28.043431053203037	22.547231270358306	20.08686210640608	29.322475570032573
26	16.27687296416938	23.0	27.743756786102065	32.979370249728554
27	28.56243213897937	29.418023887079265	27.069489685124864	14.950054288816503
28	24.006514657980457	18.147665580890337	27.897937024972858	29.94788273615635
29	17.471226927252985	30.740499457111838	35.102062975027145	16.686210640608035
30	30.05754614549403	21.351791530944624	30.55917480998914	18.031487513572202
31	15.549402823018458	26.798045602605864	29.009771986970684	28.64277958740499
32	18.093376764386537	28.879478827361567	30.320304017372422	22.706840390879478
33	17.800217155266015	27.216069489685125	29.039087947882734	25.94462540716612
34	14.27470141150923	32.07057546145494	34.62214983713355	19.03257328990228
35	24.92942453854506	27.362649294245383	28.78501628664495	18.922909880564603
36	21.65255157437568	30.35396308360478	29.12486427795874	18.868621064060804
37	25.560260586319217	28.661237785016286	30.55374592833876	15.224755700325732
38	22.463626492942453	28.897937024972855	22.07057546145494	26.567861020629753
39	18.429967426710096	28.13897937024973	32.904451682953315	20.526601520086864
40	24.469055374592834	28.36699239956569	22.990228013029316	24.17372421281216
41	15.687296416938109	28.30510314875136	29.712269272529856	26.295331161780673
42	28.461454940282298	27.34419109663409	28.17915309446254	16.01520086862106
43	25.427795874049945	21.467969598262755	23.03800217155266	30.066232356134638
44	23.087947882736156	24.051031487513573	36.40390879478828	16.457111834962
45	27.22258414766558	24.75787187839305	27.357220412595005	20.662323561346362
46	20.026058631921824	25.086862106406084	35.36264929424539	19.52442996742671
47	25.7828447339848	21.58197611292074	34.985884907709014	17.649294245385448
48	17.977198697068403	40.49402823018458	22.622149837133552	18.906623235613463
49	16.8099891422367	24.805646036916396	40.699239956568945	17.68512486427796
50	16.564603691639523	39.34961997828447	18.948968512486427	25.136807817589577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	3.0
2	3.0
3	19.0
4	35.0
5	47.5
6	60.0
7	57.0
8	54.0
9	76.5
10	99.0
11	162.0
12	225.0
13	385.0
14	545.0
15	719.5
16	894.0
17	1078.5
18	1263.0
19	1391.5
20	1520.0
21	1552.5
22	1585.0
23	1630.5
24	1676.0
25	1255.5
26	835.0
27	707.0
28	579.0
29	643.0
30	707.0
31	892.0
32	1077.0
33	1241.0
34	1405.0
35	1501.5
36	1598.0
37	1615.5
38	1633.0
39	1887.5
40	2142.0
41	2903.5
42	3665.0
43	5462.0
44	7259.0
45	10851.0
46	14443.0
47	17231.0
48	20019.0
49	16772.5
50	13526.0
51	9613.0
52	5700.0
53	3981.0
54	2262.0
55	1969.5
56	1677.0
57	1449.5
58	1222.0
59	1144.5
60	1067.0
61	998.5
62	930.0
63	794.0
64	658.0
65	616.5
66	575.0
67	495.0
68	415.0
69	347.5
70	280.0
71	227.0
72	174.0
73	139.0
74	104.0
75	102.0
76	100.0
77	69.0
78	38.0
79	30.0
80	22.0
81	17.5
82	13.0
83	11.5
84	10.0
85	5.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019543973941368076
2	0.0
3	0.0010857763300760044
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	92100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.79587404994571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.40539008329337	42.03040173724213
2	8.477606733243208	8.44299674267101
3	2.3984998473681918	3.5830618892508146
4	1.212332650124286	2.414766558089034
5	0.6693995028563953	1.6666666666666667
6	0.49932405913392347	1.49185667752443
7	0.3510531594784353	1.2236699239956568
8	0.2725568008372945	1.0857763300760044
9	0.19624089660285204	0.8794788273615635
>10	1.3017312807989185	12.15200868621064
>50	0.09812044830142602	3.4039087947882734
>100	0.0937595394880293	8.95114006514658
>500	0.015263180846888491	4.598262757871878
>1k	0.008721817626793424	8.07600434310532
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2807	3.047774158523344	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2486	2.6992399565689467	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1092	1.1856677524429968	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1053	1.1433224755700326	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	717	0.7785016286644951	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	660	0.7166123778501629	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	655	0.7111834961997828	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	589	0.6395222584147666	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	571	0.6199782844733985	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	523	0.5678610206297503	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	520	0.5646036916395223	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	468	0.50814332247557	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	390	0.4234527687296417	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	385	0.4180238870792617	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	348	0.3778501628664495	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	331	0.35939196525515743	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	307	0.33333333333333337	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	298	0.3235613463626493	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	261	0.28338762214983715	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	254	0.27578718783930506	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	237	0.25732899022801303	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	233	0.252985884907709	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	222	0.24104234527687296	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	215	0.23344191096634093	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	214	0.23235613463626492	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	203	0.22041259500542887	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	195	0.21172638436482086	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTATGCGTTGATA	191	0.20738327904451684	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA	180	0.19543973941368079	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.18892508143322476	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	174	0.18892508143322476	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	160	0.17372421281216072	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	160	0.17372421281216072	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	153	0.16612377850162866	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	145	0.15743756786102062	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	141	0.15309446254071662	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTA	140	0.15200868621064062	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	140	0.15200868621064062	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATCAGTGGTATA	136	0.14766558089033657	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	134	0.1454940282301846	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	134	0.1454940282301846	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	131	0.14223669923995658	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	130	0.14115092290988057	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	127	0.13789359391965253	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	121	0.13137893593919653	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	121	0.13137893593919653	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG	119	0.12920738327904452	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.1281216069489685	No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	115	0.1248642779587405	No Hit
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA	115	0.1248642779587405	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTTCGTTGATA	113	0.12269272529858849	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.11617806731813246	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	102	0.11074918566775244	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACA	102	0.11074918566775244	No Hit
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA	97	0.10532030401737241	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTA	96	0.10423452768729641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0010857763300760044	0.0
10	0.0	0.0	0.0	0.0010857763300760044	0.0
11	0.0	0.0	0.0	0.0010857763300760044	0.0
12	0.0	0.0	0.0	0.0010857763300760044	0.0
13	0.0	0.0	0.0	0.0010857763300760044	0.0
14	0.0	0.0	0.0	0.0010857763300760044	0.0
15	0.0	0.0	0.0	0.0010857763300760044	0.0
16	0.0	0.0	0.0	0.002171552660152009	0.0
17	0.0	0.0	0.0	0.003257328990228013	0.0
18	0.0	0.0	0.0	0.003257328990228013	0.0
19	0.0	0.0	0.0	0.003257328990228013	0.0
20	0.0	0.0	0.0	0.006514657980456026	0.0
21	0.0	0.0	0.0	0.008686210640608035	0.0
22	0.0	0.0	0.0	0.014115092290988056	0.0
23	0.0	0.0	0.0	0.02280130293159609	0.0
24	0.0	0.0	0.0	0.035830618892508145	0.0
25	0.0	0.0	0.0	0.04777415852334419	0.0
26	0.0	0.0	0.0	0.06948968512486428	0.0
27	0.0	0.0	0.0	0.09011943539630836	0.0
28	0.0	0.0	0.0	0.1281216069489685	0.0
29	0.0	0.0	0.0	0.1748099891422367	0.0
30	0.0	0.0	0.0	0.23778501628664495	0.0
31	0.0	0.0	0.0	0.3344191096634093	0.0
32	0.0	0.0	0.0	0.48642779587404994	0.0
33	0.0	0.0	0.0	0.6015200868621065	0.0
34	0.0	0.0	0.0	0.7741585233441911	0.0
35	0.0	0.0	0.0	0.9706840390879479	0.0
36	0.0	0.0	0.0	1.239956568946797	0.0
37	0.0	0.0	0.0	1.6319218241042346	0.0
38	0.0	0.0	0.0	1.9522258414766558	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTGT	20	7.818774E-4	44.000004	38
TGGACAT	20	7.818774E-4	44.000004	10
TTTGATA	55	7.6397555E-11	40.0	44
ACAGTGG	30	1.291822E-4	36.666664	6
CTAAATT	30	1.291822E-4	36.666664	19
ATTTCTA	30	1.291822E-4	36.666664	15
CTTCAGG	25	0.002341562	35.2	1
CTCTCAG	25	0.002341562	35.2	38
AGTGGAC	25	0.002341562	35.2	8
GGACATT	25	0.002341562	35.2	11
GACATTT	25	0.002341562	35.2	12
TTCCTCG	25	0.002341562	35.2	43
CACTCTT	25	0.002341562	35.2	41
TCTCAGT	25	0.002341562	35.2	39
GTGGACA	25	0.002341562	35.2	9
TGTTATA	65	4.5656634E-10	33.846157	44
CTACAGT	40	1.801972E-5	33.0	4
CCTACAG	40	1.801972E-5	33.0	3
TCCTACA	40	1.801972E-5	33.0	2
AATTTTC	35	3.1950118E-4	31.428572	22
>>END_MODULE