FastQCFastQC Report
Thu 2 Feb 2017
SRR4062507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980649
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69510.7088163043045983No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54370.5544287507558769No Hit
GTACATGGAAGCAGTGGTATCAACG45540.4643863400666293No Hit
TATCAACGCAGAGTACTTTTTTTTT43740.44603114875964794No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41520.4233930794810376No Hit
TCCATGTACTCTGCGTTGATACCAC39740.4052418347441337No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29050.296232393037672No Hit
GTCCTAAAGTGTGTATTTCTCATTT28470.2903179425054224No Hit
GTATTAGAGGCACTGCCTGCCCAGT27450.2799166674314663No Hit
GTCCTACAGTGGACATTTCTAAATT25790.2629891021150279No Hit
CCATAGGGTCTTCTCGTCTTATTAT25300.25799241114812743No Hit
CTCTAATACTTGTAATGCTAGAGGT23640.24106484583168902No Hit
CTGTAGGACGTGGAATATGGCAAGA23150.23606815486478852No Hit
CTTTAGGACGTGAAATATGGCGAGG22600.2304596241876553No Hit
GGTATCAACGCAGAGTACTTTTTTT22320.22760437220656934No Hit
GTCCTACAGTGTGCATTTCTCATTT20950.2136340321562557No Hit
GAGTACATGGAAGCAGTGGTATCAA20890.213022192446023No Hit
CCATTGGGATGTCCTGATCCAACAT20660.21067680689013094No Hit
GTACATGGGGTGGTATCAACGCAAA20310.20710774191377343No Hit
GTTATAGATTAACCCAATTTTAAGT17820.18171639393911582No Hit
GATATATTTTGATCAACGGACCAAG17550.17896311524306863No Hit
CTTCTACACCATTGGGATGTCCTGA17470.17814732896275834No Hit
CATGTACTCTGCGTTGATACCACTG17330.17671970297221534No Hit
ATCTATAACTTTATAGATGCAACAC17100.17437431741632328No Hit
GTCAGGATACCGCGGCCGTTAAACT16590.1691736798793452No Hit
CTGTTAGTATGAGTAACAAGAATTC16000.16315725606205686No Hit
CTGTAGGACCTGGAATATGGCGAGA15460.15765069866996245No Hit
ATCCTGACCGTGCAAAGGTAGCATA15350.15652899253453578No Hit
GTTATATAATTTAAGCTCCATAGGG15210.15510136654399279No Hit
GTATCCTGACCGTGCAAAGGTAGCA15100.15397966040856617No Hit
GCCTAAAGGAAAGATCCAAAAAGAT14960.15255203441802317No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14520.1480652098763166No Hit
ATCGTAAATAGATAGAAACCGACCT13960.14235470591414462No Hit
CCTCTAGCATTACAAGTATTAGAGG13860.14133497306375675No Hit
CTATAGAACTAGTACCGCAAGGGAA13740.14011129364329133No Hit
AAATATATCTGGGTCAATAAGATAT13720.13990734707321376No Hit
GTTCATGCTAGTCCCTAATTAAGGA13680.13949945393305863No Hit
GTACATGGGTGGTATCAACGCAAAA13020.13276921712049877No Hit
GGTCAGGATACCGCGGCCGTTAAAC12920.1317494842701109No Hit
TACTAACAGTGTTGCATCTATAAAG12800.1305258048496455No Hit
GATTAAACCTTGTACCTTTTGCATA12670.12920015214414127No Hit
TCCCAATGGTGTAGAAGCTATTAAT12500.12746660629848192No Hit
TGCTAGAGGTGATGTTTTTGGTAAA12330.12573306045282256No Hit
ACCTATAACTTCTCTGTTAACCCAA12200.12440740774731837No Hit
GATATACACTGTTCTACAAATCCCG12130.12369359475204686No Hit
GTACATGGGAGAAATCGTAAATAGA12100.1233876748969305No Hit
CACTATAAATAATCCACCTATAACT12060.12297978175677536No Hit
CCAATAAAGAAAGCGTTCAAGCTCA12060.12297978175677536No Hit
GTTATACGCGTATGCCTGGAGAATT11760.11992058320561179No Hit
GTGTAGAAGCTATTAATGGTTCGTT11690.11920677021034029No Hit
GTATCAACGCAGAGTACATGGGAGA11640.11869690378514637No Hit
CCTATAGTCTGATTAACTAACAATG11630.11859493050010758No Hit
ATCCTAGCCCTAGCCCTACACAAAT11630.11859493050010758No Hit
TTGTAGAACAGTGTATATCAATGAG11590.11818703735995244No Hit
CTGAAGGACCTGGAATATGGCGAGA11590.11818703735995244No Hit
GATTAAAGATAAGAGACAGTTGGAC11500.11726927779460337No Hit
GACTATAGGCAATAATCACACTATA11410.1163515182292543No Hit
GCGTTGATACCACTGCTTCCATGTA11340.11563770523398281No Hit
GCTTCCATGTACTCTGCGTTGATAC11300.11522981209382765No Hit
ATAAATAATCCACCTATAACTTCTC11260.11482191895367251No Hit
AGCATGAACGGCTAAACGAGGGTCC10980.11196666697258653No Hit
GATAGAAACCGACCTGGATTGCTCC10910.11125285397731502No Hit
CCTATAACTTCTCTGTTAACCCAAC10900.11115088069227623No Hit
CATGGAAGCAGTGGTATCAACGCAG10730.10941733484661688No Hit
GTCCTGATCCAACATCGAGGTCGTA10700.10911141499150054No Hit
GTCCTTCAGTGTGCATTTCTCATTT10670.10880549513638416No Hit
GTTAGTATGAGTAACAAGAATTCCA10500.10707194929072482No Hit
ATTTAGAAATGTCCACTGTAGGACG10470.10676602943560846No Hit
GTACATGGGAGTGGTATCAACGCAA10460.10666405615056967No Hit
GACCTGGATTGCTCCGGTCTGAACT10370.10574629658522061No Hit
GTGTATATCAATGAGTTACAATGAG10330.10533840344506547No Hit
ATTATAACCTAGACTTACAAGTCAA10320.10523643016002668No Hit
CTGTTAACCCAACACCGGAATGCCT10180.10380880416948368No Hit
CTATTATATAAATCAAAACATTTAT10180.10380880416948368No Hit
GTTCTATAGCTCCTAGATGTACGAA10160.10360485759940612No Hit
GGTTTAATCTTTGCTTGTTCTTACT10160.10360485759940612No Hit
CTTTATTGGTGGCTGCTTTTAGGCC10070.10268709803405705No Hit
ACCCTATGGAGCTTAAATTATATAA10040.10238117817894067No Hit
CCTTTAGGCATTCCGGTGTTGGGTT10010.10207525832382433No Hit
GTGTATATCAATGAGTTACAATGAA9980.10176933846870798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG250.00604082318.9943854
TCCGAAC250.00604616918.9914783
ACACGTC350.002169272616.285896
GTGTTAA350.002181139516.2725941
AAGCGTT1800.014.78020111
AAAGCGT2050.014.367475510
CTTGTCT400.005269733714.25306514
TACACGT400.005285825614.2465165
GAAAGCG2200.013.8190369
CGTTCAA2000.013.3028614
ACTTGCT2150.013.2566348
GCGTTCA2100.013.12253913
AGCGTTC2100.013.12186912
AACTTGC2100.013.1198597
CCAACGA1251.0186341E-1012.92343819
TAGAAAT2900.012.7720864
TAGGACC6850.012.6167084
AAGATCC3100.012.56657711
CTTGCTA2200.012.5235019
AGATCCA3050.012.46169612
Produced by FastQC (version 0.11.5)