##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062507_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 980649 Sequences flagged as poor quality 0 Sequence length 25 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93061941632531 32.0 32.0 32.0 32.0 32.0 2 30.811099588129903 32.0 32.0 32.0 32.0 32.0 3 30.83571084047401 32.0 32.0 32.0 32.0 32.0 4 30.80210554438948 32.0 32.0 32.0 32.0 32.0 5 30.792307951162954 32.0 32.0 32.0 32.0 32.0 6 34.33171909623117 36.0 36.0 36.0 32.0 36.0 7 34.109628419546645 36.0 36.0 36.0 32.0 36.0 8 34.070846959513545 36.0 36.0 36.0 32.0 36.0 9 34.147436034707624 36.0 36.0 36.0 32.0 36.0 10 34.01054607713871 36.0 36.0 36.0 32.0 36.0 11 34.30948892009271 36.0 36.0 36.0 32.0 36.0 12 34.126767069563115 36.0 36.0 36.0 32.0 36.0 13 34.21158029019558 36.0 36.0 36.0 32.0 36.0 14 34.15366762215635 36.0 36.0 36.0 32.0 36.0 15 34.15930368562044 36.0 36.0 36.0 32.0 36.0 16 34.152798809767816 36.0 36.0 36.0 32.0 36.0 17 34.11804325502805 36.0 36.0 36.0 32.0 36.0 18 34.17604260035956 36.0 36.0 36.0 32.0 36.0 19 34.10544853459291 36.0 36.0 36.0 32.0 36.0 20 34.02276451615206 36.0 36.0 36.0 32.0 36.0 21 33.96892670058298 36.0 36.0 36.0 32.0 36.0 22 33.94693616166437 36.0 36.0 36.0 32.0 36.0 23 33.96909393677044 36.0 36.0 36.0 32.0 36.0 24 33.9474205347683 36.0 36.0 36.0 32.0 36.0 25 33.517545013557346 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 39.0 5 89.0 6 170.0 7 40.0 8 152.0 9 174.0 10 124.0 11 37.0 12 72.0 13 51.0 14 212.0 15 306.0 16 469.0 17 751.0 18 1072.0 19 1521.0 20 2370.0 21 3170.0 22 4217.0 23 5714.0 24 7037.0 25 8830.0 26 11188.0 27 14059.0 28 18239.0 29 24267.0 30 31527.0 31 43930.0 32 63365.0 33 92828.0 34 214604.0 35 430023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17831031391598 21.877845982199766 12.206937134197272 23.73690656968698 2 13.242912502549459 22.61594941872323 43.15102998164389 20.990108097083418 3 17.482380559518575 28.51539833549699 31.71784828160103 22.284372823383404 4 9.799216119823724 18.436992290723065 42.41213984193892 29.351651747514296 5 9.763701131508828 41.339550579219896 37.5582994315933 11.33844885767798 6 26.123071084477473 42.34238645846723 20.08061504936677 11.453927407688527 7 24.391259359942204 35.251955664129866 23.804815229791814 16.551969746136123 8 23.567987425235266 37.96027517504644 22.06126115091759 16.410476248800702 9 26.284045580555997 15.545219226796336 22.933584834788856 35.23715035785881 10 14.176135290460183 28.558403941751752 36.575347920237924 20.69011284755014 11 30.97183765814772 24.282158405005188 28.20195535517385 16.544048581673245 12 22.53657610116325 27.912915795320874 33.06369265455496 16.48681544896092 13 26.574676733489543 23.749329984532213 29.28097768657059 20.39501559540765 14 19.36929762225653 25.542976630971925 29.23364535600807 25.854080390763475 15 21.46398185259521 33.2456045425682 26.746595830262144 18.54381777457444 16 20.01031080439587 29.88836767715953 30.619413917544193 19.48190760090041 17 17.71859429878298 30.499571183533448 31.465020828228376 20.3168136894552 18 18.00337777124279 28.373891094446897 35.623846166413436 17.998884967896878 19 21.19086956432946 28.92952033966495 30.26299935261069 19.616610743394894 20 20.350192963469667 30.06626100095971 31.923304678087927 17.660241357482693 21 21.14873095565569 27.591108061497366 29.101892155991827 22.158268826855114 22 20.071287411848214 31.598707022014327 29.334157189764436 18.995848376373022 23 19.634401508877524 30.77656746702394 30.964462339842598 18.62456868425594 24 20.285357774483014 30.74074981722235 30.784350965760332 18.1895414425343 25 20.105276056079155 30.244350729580425 31.736493316859487 17.913879897480932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 311.0 1 311.0 2 983.0 3 1655.0 4 1655.0 5 1655.0 6 5527.5 7 9400.0 8 9400.0 9 9400.0 10 9306.5 11 9213.0 12 9213.0 13 9213.0 14 8961.0 15 8709.0 16 8709.0 17 8709.0 18 14386.0 19 20063.0 20 20063.0 21 20063.0 22 31109.5 23 42156.0 24 42156.0 25 42156.0 26 62185.0 27 82214.0 28 82214.0 29 82214.0 30 99181.0 31 116148.0 32 116148.0 33 116148.0 34 120172.5 35 124197.0 36 124197.0 37 124197.0 38 119196.0 39 114195.0 40 114195.0 41 114195.0 42 109135.5 43 104076.0 44 104076.0 45 104076.0 46 119083.0 47 134090.0 48 134090.0 49 134090.0 50 113106.0 51 92122.0 52 92122.0 53 92122.0 54 71596.5 55 51071.0 56 51071.0 57 51071.0 58 40198.0 59 29325.0 60 29325.0 61 29325.0 62 23489.0 63 17653.0 64 17653.0 65 17653.0 66 14354.5 67 11056.0 68 11056.0 69 11056.0 70 8594.5 71 6133.0 72 6133.0 73 6133.0 74 4869.0 75 3605.0 76 3605.0 77 3605.0 78 2687.5 79 1770.0 80 1770.0 81 1770.0 82 1245.0 83 720.0 84 720.0 85 720.0 86 493.0 87 266.0 88 266.0 89 266.0 90 166.0 91 66.0 92 66.0 93 66.0 94 55.5 95 45.0 96 45.0 97 45.0 98 217.5 99 390.0 100 390.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010197328503878554 2 0.004996690966900491 3 0.00571050396217199 4 0.014480206475507545 5 0.03722024903915672 6 0.04435837899187171 7 0.06883196740118024 8 0.09269371610025605 9 0.09514107494118691 10 0.12094031605599964 11 0.12083834277096085 12 0.13470670953623567 13 0.12175610233630993 14 0.12573306045282256 15 0.11370021281824588 16 0.11155877383243137 17 0.12328570161189172 18 0.13307513697561513 19 0.13572644238662354 20 0.121246235911116 21 0.13154553770003333 22 0.1543875535487213 23 0.14051918678344646 24 0.13429881639608054 25 0.13450276296615812 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 980649.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.119115916967 #Duplication Level Percentage of deduplicated Percentage of total 1 72.71893464059096 22.629489564388972 2 14.471267940330476 9.006661290012646 3 5.208101460256362 4.862145391471284 4 2.235046733386707 2.7821071350439754 5 1.221478810773324 1.9005670351287036 6 0.727856719217597 1.359015456976542 7 0.506543466151336 1.1034229388101973 8 0.3530158395071665 0.8788432664119146 9 0.2807846519673236 0.7863993119048741 >10 1.7512640349749513 10.586290171101146 >50 0.20252328583907322 4.497173640450467 >100 0.2570993388583523 17.29717446826936 >500 0.040110127034166144 8.420076074669963 >1k 0.025315408046154043 12.623208133377739 >5k 6.575430661338712E-4 1.2674261219821807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6951 0.7088163043045983 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5437 0.5544287507558769 No Hit GTACATGGAAGCAGTGGTATCAACG 4554 0.4643863400666293 No Hit TATCAACGCAGAGTACTTTTTTTTT 4374 0.44603114875964794 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4152 0.4233930794810376 No Hit TCCATGTACTCTGCGTTGATACCAC 3974 0.4052418347441337 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2905 0.296232393037672 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2847 0.2903179425054224 No Hit GTATTAGAGGCACTGCCTGCCCAGT 2745 0.2799166674314663 No Hit GTCCTACAGTGGACATTTCTAAATT 2579 0.2629891021150279 No Hit CCATAGGGTCTTCTCGTCTTATTAT 2530 0.25799241114812743 No Hit CTCTAATACTTGTAATGCTAGAGGT 2364 0.24106484583168902 No Hit CTGTAGGACGTGGAATATGGCAAGA 2315 0.23606815486478852 No Hit CTTTAGGACGTGAAATATGGCGAGG 2260 0.2304596241876553 No Hit GGTATCAACGCAGAGTACTTTTTTT 2232 0.22760437220656934 No Hit GTCCTACAGTGTGCATTTCTCATTT 2095 0.2136340321562557 No Hit GAGTACATGGAAGCAGTGGTATCAA 2089 0.213022192446023 No Hit CCATTGGGATGTCCTGATCCAACAT 2066 0.21067680689013094 No Hit GTACATGGGGTGGTATCAACGCAAA 2031 0.20710774191377343 No Hit GTTATAGATTAACCCAATTTTAAGT 1782 0.18171639393911582 No Hit GATATATTTTGATCAACGGACCAAG 1755 0.17896311524306863 No Hit CTTCTACACCATTGGGATGTCCTGA 1747 0.17814732896275834 No Hit CATGTACTCTGCGTTGATACCACTG 1733 0.17671970297221534 No Hit ATCTATAACTTTATAGATGCAACAC 1710 0.17437431741632328 No Hit GTCAGGATACCGCGGCCGTTAAACT 1659 0.1691736798793452 No Hit CTGTTAGTATGAGTAACAAGAATTC 1600 0.16315725606205686 No Hit CTGTAGGACCTGGAATATGGCGAGA 1546 0.15765069866996245 No Hit ATCCTGACCGTGCAAAGGTAGCATA 1535 0.15652899253453578 No Hit GTTATATAATTTAAGCTCCATAGGG 1521 0.15510136654399279 No Hit GTATCCTGACCGTGCAAAGGTAGCA 1510 0.15397966040856617 No Hit GCCTAAAGGAAAGATCCAAAAAGAT 1496 0.15255203441802317 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1452 0.1480652098763166 No Hit ATCGTAAATAGATAGAAACCGACCT 1396 0.14235470591414462 No Hit CCTCTAGCATTACAAGTATTAGAGG 1386 0.14133497306375675 No Hit CTATAGAACTAGTACCGCAAGGGAA 1374 0.14011129364329133 No Hit AAATATATCTGGGTCAATAAGATAT 1372 0.13990734707321376 No Hit GTTCATGCTAGTCCCTAATTAAGGA 1368 0.13949945393305863 No Hit GTACATGGGTGGTATCAACGCAAAA 1302 0.13276921712049877 No Hit GGTCAGGATACCGCGGCCGTTAAAC 1292 0.1317494842701109 No Hit TACTAACAGTGTTGCATCTATAAAG 1280 0.1305258048496455 No Hit GATTAAACCTTGTACCTTTTGCATA 1267 0.12920015214414127 No Hit TCCCAATGGTGTAGAAGCTATTAAT 1250 0.12746660629848192 No Hit TGCTAGAGGTGATGTTTTTGGTAAA 1233 0.12573306045282256 No Hit ACCTATAACTTCTCTGTTAACCCAA 1220 0.12440740774731837 No Hit GATATACACTGTTCTACAAATCCCG 1213 0.12369359475204686 No Hit GTACATGGGAGAAATCGTAAATAGA 1210 0.1233876748969305 No Hit CACTATAAATAATCCACCTATAACT 1206 0.12297978175677536 No Hit CCAATAAAGAAAGCGTTCAAGCTCA 1206 0.12297978175677536 No Hit GTTATACGCGTATGCCTGGAGAATT 1176 0.11992058320561179 No Hit GTGTAGAAGCTATTAATGGTTCGTT 1169 0.11920677021034029 No Hit GTATCAACGCAGAGTACATGGGAGA 1164 0.11869690378514637 No Hit CCTATAGTCTGATTAACTAACAATG 1163 0.11859493050010758 No Hit ATCCTAGCCCTAGCCCTACACAAAT 1163 0.11859493050010758 No Hit TTGTAGAACAGTGTATATCAATGAG 1159 0.11818703735995244 No Hit CTGAAGGACCTGGAATATGGCGAGA 1159 0.11818703735995244 No Hit GATTAAAGATAAGAGACAGTTGGAC 1150 0.11726927779460337 No Hit GACTATAGGCAATAATCACACTATA 1141 0.1163515182292543 No Hit GCGTTGATACCACTGCTTCCATGTA 1134 0.11563770523398281 No Hit GCTTCCATGTACTCTGCGTTGATAC 1130 0.11522981209382765 No Hit ATAAATAATCCACCTATAACTTCTC 1126 0.11482191895367251 No Hit AGCATGAACGGCTAAACGAGGGTCC 1098 0.11196666697258653 No Hit GATAGAAACCGACCTGGATTGCTCC 1091 0.11125285397731502 No Hit CCTATAACTTCTCTGTTAACCCAAC 1090 0.11115088069227623 No Hit CATGGAAGCAGTGGTATCAACGCAG 1073 0.10941733484661688 No Hit GTCCTGATCCAACATCGAGGTCGTA 1070 0.10911141499150054 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1067 0.10880549513638416 No Hit GTTAGTATGAGTAACAAGAATTCCA 1050 0.10707194929072482 No Hit ATTTAGAAATGTCCACTGTAGGACG 1047 0.10676602943560846 No Hit GTACATGGGAGTGGTATCAACGCAA 1046 0.10666405615056967 No Hit GACCTGGATTGCTCCGGTCTGAACT 1037 0.10574629658522061 No Hit GTGTATATCAATGAGTTACAATGAG 1033 0.10533840344506547 No Hit ATTATAACCTAGACTTACAAGTCAA 1032 0.10523643016002668 No Hit CTGTTAACCCAACACCGGAATGCCT 1018 0.10380880416948368 No Hit CTATTATATAAATCAAAACATTTAT 1018 0.10380880416948368 No Hit GTTCTATAGCTCCTAGATGTACGAA 1016 0.10360485759940612 No Hit GGTTTAATCTTTGCTTGTTCTTACT 1016 0.10360485759940612 No Hit CTTTATTGGTGGCTGCTTTTAGGCC 1007 0.10268709803405705 No Hit ACCCTATGGAGCTTAAATTATATAA 1004 0.10238117817894067 No Hit CCTTTAGGCATTCCGGTGTTGGGTT 1001 0.10207525832382433 No Hit GTGTATATCAATGAGTTACAATGAA 998 0.10176933846870798 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0197328503878554E-4 0.0 5 0.0 0.0 0.0 2.0394657007757108E-4 0.0 6 0.0 0.0 0.0 2.0394657007757108E-4 0.0 7 0.0 0.0 0.0 5.098664251939277E-4 0.0 8 0.0 0.0 0.0 6.118397102327132E-4 0.0 9 0.0 0.0 0.0 9.177595653490698E-4 0.0 10 0.0 0.0 0.0 9.177595653490698E-4 0.0 11 0.0 0.0 0.0 9.177595653490698E-4 4.0789314015514215E-4 12 0.0 0.0 0.0 0.0010197328503878554 4.0789314015514215E-4 13 0.0 0.0 0.0 0.0010197328503878554 4.0789314015514215E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGGG 25 0.006040823 18.994385 4 TCCGAAC 25 0.006046169 18.991478 3 ACACGTC 35 0.0021692726 16.28589 6 GTGTTAA 35 0.0021811395 16.272594 1 AAGCGTT 180 0.0 14.780201 11 AAAGCGT 205 0.0 14.3674755 10 CTTGTCT 40 0.0052697337 14.253065 14 TACACGT 40 0.0052858256 14.246516 5 GAAAGCG 220 0.0 13.819036 9 CGTTCAA 200 0.0 13.30286 14 ACTTGCT 215 0.0 13.256634 8 GCGTTCA 210 0.0 13.122539 13 AGCGTTC 210 0.0 13.121869 12 AACTTGC 210 0.0 13.119859 7 CCAACGA 125 1.0186341E-10 12.923438 19 TAGAAAT 290 0.0 12.772086 4 TAGGACC 685 0.0 12.616708 4 AAGATCC 310 0.0 12.566577 11 CTTGCTA 220 0.0 12.523501 9 AGATCCA 305 0.0 12.461696 12 >>END_MODULE