##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062507_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980649
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.992445819044327	32.0	32.0	32.0	32.0	32.0
2	31.378514636735467	32.0	32.0	32.0	32.0	32.0
3	31.490662816155425	32.0	32.0	32.0	32.0	32.0
4	31.596717072061463	32.0	32.0	32.0	32.0	32.0
5	31.541676991461777	32.0	32.0	32.0	32.0	32.0
6	35.081413431309265	36.0	36.0	36.0	36.0	36.0
7	35.10985480023943	36.0	36.0	36.0	36.0	36.0
8	35.04003981037048	36.0	36.0	36.0	36.0	36.0
9	35.16191930038168	36.0	36.0	36.0	36.0	36.0
10	35.07043090851059	36.0	36.0	36.0	36.0	36.0
11	35.19069718115248	36.0	36.0	36.0	36.0	36.0
12	35.09530627166295	36.0	36.0	36.0	36.0	36.0
13	35.13831860329231	36.0	36.0	36.0	36.0	36.0
14	35.09959832723023	36.0	36.0	36.0	36.0	36.0
15	35.0879387018189	36.0	36.0	36.0	36.0	36.0
16	35.09038911985838	36.0	36.0	36.0	36.0	36.0
17	35.1053027127953	36.0	36.0	36.0	36.0	36.0
18	35.10500393107014	36.0	36.0	36.0	36.0	36.0
19	35.0549615611702	36.0	36.0	36.0	36.0	36.0
20	35.0040738327373	36.0	36.0	36.0	36.0	36.0
21	34.96926423215646	36.0	36.0	36.0	36.0	36.0
22	34.96165804482542	36.0	36.0	36.0	36.0	36.0
23	34.967691804101165	36.0	36.0	36.0	36.0	36.0
24	34.95449238208574	36.0	36.0	36.0	36.0	36.0
25	34.95404471936442	36.0	36.0	36.0	36.0	36.0
26	34.903973796944676	36.0	36.0	36.0	36.0	36.0
27	34.88110730750758	36.0	36.0	36.0	36.0	36.0
28	34.861779291061325	36.0	36.0	36.0	36.0	36.0
29	34.828455441243506	36.0	36.0	36.0	32.0	36.0
30	34.78481393444545	36.0	36.0	36.0	32.0	36.0
31	34.77529880721848	36.0	36.0	36.0	32.0	36.0
32	34.727113370839106	36.0	36.0	36.0	32.0	36.0
33	34.690749697394274	36.0	36.0	36.0	32.0	36.0
34	34.63572695225305	36.0	36.0	36.0	32.0	36.0
35	34.57207216853329	36.0	36.0	36.0	32.0	36.0
36	34.510101983482365	36.0	36.0	36.0	32.0	36.0
37	34.463684763865565	36.0	36.0	36.0	32.0	36.0
38	34.42396820880866	36.0	36.0	36.0	32.0	36.0
39	34.31206578500564	36.0	36.0	36.0	32.0	36.0
40	34.29397062557551	36.0	36.0	36.0	32.0	36.0
41	34.24703028300646	36.0	36.0	36.0	32.0	36.0
42	34.19052280683506	36.0	36.0	36.0	32.0	36.0
43	34.168593451887475	36.0	36.0	36.0	32.0	36.0
44	34.10494988522907	36.0	36.0	36.0	32.0	36.0
45	33.997796357310314	36.0	36.0	36.0	32.0	36.0
46	33.92013554289047	36.0	36.0	36.0	32.0	36.0
47	33.84361886872877	36.0	36.0	36.0	32.0	36.0
48	33.76150997961554	36.0	36.0	36.0	27.0	36.0
49	33.723948120071505	36.0	36.0	36.0	27.0	36.0
50	33.19957191614941	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	26.0
22	72.0
23	226.0
24	719.0
25	1894.0
26	4151.0
27	8050.0
28	13687.0
29	20278.0
30	27686.0
31	37570.0
32	53344.0
33	79748.0
34	161553.0
35	571635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.517942996081565	22.759241769964937	12.632815703104761	24.089999530848736
2	12.961089605266915	23.156661350258815	42.518651617561275	21.36359742691299
3	17.18683716756404	28.732664382444117	32.219162995594715	21.86133545439713
4	9.752123085959488	18.909945260684772	42.55278142615903	28.78515022719671
5	9.67532725776501	41.68596511086026	37.35668929453861	11.282018336836115
6	26.304851485956227	42.456518546919995	19.803436095316567	11.435193871807213
7	24.426986618045802	35.27245732163088	23.777722712203857	16.52283334811946
8	23.804541686169056	38.06734111797391	21.859809167194378	16.268308028662652
9	26.164203501966554	15.35748264669622	22.927265514980384	35.551048336356835
10	14.228332461461745	28.62308532410679	36.450860603539084	20.697721610892376
11	31.168746411815036	24.017767825185157	28.296260945557485	16.517224817442326
12	22.477971221099498	28.0515250614644	32.935535548397034	16.534968169039075
13	26.83131273269029	23.520240167480924	29.421434172675443	20.227012927153346
14	19.407147715441507	25.58938009420292	29.255421664632298	25.748050525723272
15	21.5422643575836	33.28142893124859	26.50968899167796	18.666617719489846
16	20.19397358280078	29.87847843621928	30.389262620978556	19.538285360001385
17	17.842469629806384	30.54426201423751	31.176802301333094	20.436466054623008
18	18.176432138308407	28.241297344921577	35.43337116542208	18.148899351347932
19	21.55062616695678	28.721081651029063	30.31125305792389	19.41703912409027
20	20.62603439151011	29.99574771401388	31.619672278256544	17.75854561621946
21	21.393077441571855	27.424389358475864	29.103787389779622	22.078745810172652
22	20.207739976281015	31.26144012791529	29.318543128071305	19.21227676773239
23	19.708274826161045	30.6438899137204	30.84569504481216	18.80214021530639
24	20.496528319510855	30.66204115845731	30.452180137847485	18.38925038418435
25	20.506623674729692	30.00359965696187	31.298354457099332	18.191422211209108
26	17.301297406105547	30.247213834919528	33.41205670938328	19.03943204959165
27	18.874541247683933	31.92365464095716	31.107562440791764	18.094241670567147
28	17.94250542242943	30.71476134682236	31.254200024677537	20.088533206070675
29	19.3090494152342	29.751419723060952	30.734442190834844	20.205088670870005
30	19.746514808050584	29.05524810610116	31.4399953500182	19.758241735830048
31	19.631896835666993	28.700890940591385	31.304778774056775	20.362433449684854
32	17.430089665109534	31.32843657618577	31.970460378789966	19.27101337991473
33	18.450026462067466	31.356989095996628	31.854720700270946	18.33826374166496
34	18.24750751798044	30.48052871108827	33.492411657993834	17.779552112937452
35	19.661978954753433	31.425005277117503	30.24894738076519	18.664068387363876
36	18.50682558183407	29.91182370042696	31.87358575800312	19.707764959735847
37	19.480568480669433	30.315637909180555	29.12255047422676	21.08124313592325
38	18.858939335072996	31.912743499458013	30.000132565270548	19.22818460019844
39	18.87627479352959	30.61666304661505	29.493631258482907	21.01343090137246
40	20.53374856855001	30.3838580368715	31.103687455960287	17.9787059386182
41	20.217019545219543	28.925334140961752	31.656484634155547	19.201161679663162
42	18.936541004987514	32.32247215874386	30.297486664443653	18.443500171824986
43	17.13671252405295	32.206630506939796	30.890461316944183	19.76619565206307
44	17.79892703709482	30.66520233029351	31.56858366245211	19.967286970159556
45	18.222728009716015	31.113170971468897	31.121430807557037	19.542670211258052
46	19.85413711559817	29.63349706877194	29.645733887933172	20.866631927696712
47	20.839974343521483	29.641696468359218	29.59968347492324	19.918645713196057
48	18.36181957050892	31.63313275188166	28.966123454977264	21.038924222632154
49	18.802038242021354	29.266638725986567	30.981829380338937	20.94949365165314
50	16.861690574303346	32.08874938943496	29.39400335900001	21.655556677261693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	37.5
2	48.0
3	124.5
4	201.0
5	236.0
6	271.0
7	278.0
8	285.0
9	418.5
10	552.0
11	848.0
12	1144.0
13	2150.0
14	3156.0
15	4590.0
16	6024.0
17	8574.5
18	11125.0
19	12523.0
20	13921.0
21	16367.5
22	18814.0
23	18581.5
24	18349.0
25	21788.0
26	25227.0
27	32857.5
28	40488.0
29	45540.0
30	50592.0
31	50904.0
32	51216.0
33	59598.0
34	67980.0
35	76759.5
36	85539.0
37	91698.5
38	97858.0
39	90486.5
40	83115.0
41	80506.5
42	77898.0
43	67148.0
44	56398.0
45	54758.5
46	53119.0
47	50916.5
48	48714.0
49	41190.5
50	33667.0
51	31717.5
52	29768.0
53	25996.5
54	22225.0
55	20016.5
56	17808.0
57	17769.0
58	17730.0
59	14555.0
60	11380.0
61	10168.0
62	8956.0
63	7872.5
64	6789.0
65	6326.5
66	5864.0
67	5241.0
68	4618.0
69	3980.5
70	3343.0
71	2812.0
72	2281.0
73	1920.5
74	1560.0
75	1316.5
76	1073.0
77	870.5
78	668.0
79	515.5
80	363.0
81	292.0
82	221.0
83	193.0
84	165.0
85	116.5
86	68.0
87	40.5
88	13.0
89	11.5
90	10.0
91	7.5
92	5.0
93	4.5
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015805859181011756
2	0.0037730115464350653
3	9.1775956534907E-4
4	1.0197328503878554E-4
5	0.0
6	1.0197328503878554E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.0394657007757108E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	980649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.579310496569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9881751888339	30.83547755055372
2	13.245609826709186	10.749954277488753
3	4.456927388222304	5.425771210420056
4	1.95343036016069	3.17075428473541
5	1.0259926789841394	2.0817037743852014
6	0.6267032538549078	1.5258711556433044
7	0.42523693833705145	1.2079075228772704
8	0.3027530998638116	0.9828409634537898
9	0.22571027808292624	0.8243250710934596
>10	1.3981245664508377	10.60905111964019
>50	0.1462432644513457	4.236208910772518
>100	0.16771164484927567	14.654945490231777
>500	0.027278400064648697	7.825543897531498
>1k	0.010103111135055071	5.8696447711730535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	2683	0.27359432375906156	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2439	0.2487128422095979	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2271	0.23158133032308198	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	2255	0.2299497577624614	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1861	0.1897722834571799	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1722	0.1755979968367887	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	1713	0.1746802372714396	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1709	0.17427234413128448	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1705	0.17386445099112935	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	1607	0.16387106905732837	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1592	0.16234146978174657	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1524	0.15540728639910917	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	1479	0.15081848857236382	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	1438	0.1466375838857736	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	1411	0.1438843051897264	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	1403	0.1430685189094161	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	1402	0.14296654562437733	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	1352	0.13786788137243805	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	1331	0.13572644238662354	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1303	0.13287119040553755	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	1284	0.13093369798980065	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	1265	0.12899620557406372	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	1250	0.12746660629848192	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	1235	0.12593700702290014	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1216	0.12399951460716323	No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA	1203	0.12267386190165899	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	1173	0.11961466335049543	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	1173	0.11961466335049543	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	1172	0.11951269006545664	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	1169	0.11920677021034029	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT	1168	0.1191047969253015	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	1145	0.11675941136940944	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT	1133	0.11553573194894402	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	1128	0.11502586552375009	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	1113	0.1134962662481683	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	1106	0.1127824532528968	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	1046	0.10666405615056967	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	1043	0.10635813629545332	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA	1042	0.10625616301041453	No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA	1004	0.10238117817894067	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG	999	0.10187131175374675	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.1015653918986304	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	993	0.10125947204351404	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	989	0.10085157890335888	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	987	0.10064763233328133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0197328503878554E-4	0.0
3	0.0	0.0	0.0	1.0197328503878554E-4	0.0
4	0.0	0.0	0.0	1.0197328503878554E-4	0.0
5	0.0	0.0	0.0	2.0394657007757108E-4	0.0
6	0.0	0.0	0.0	2.0394657007757108E-4	0.0
7	0.0	0.0	0.0	2.0394657007757108E-4	0.0
8	0.0	0.0	0.0	2.0394657007757108E-4	0.0
9	0.0	0.0	0.0	3.059198551163566E-4	0.0
10	0.0	0.0	0.0	3.059198551163566E-4	0.0
11	0.0	0.0	0.0	3.059198551163566E-4	1.0197328503878554E-4
12	0.0	0.0	0.0	4.0789314015514215E-4	1.0197328503878554E-4
13	0.0	0.0	0.0	4.0789314015514215E-4	1.0197328503878554E-4
14	0.0	0.0	0.0	4.0789314015514215E-4	1.0197328503878554E-4
15	0.0	0.0	0.0	4.0789314015514215E-4	1.0197328503878554E-4
16	0.0	0.0	0.0	5.098664251939277E-4	1.0197328503878554E-4
17	0.0	0.0	0.0	6.118397102327132E-4	1.0197328503878554E-4
18	0.0	0.0	0.0	9.177595653490698E-4	1.0197328503878554E-4
19	0.0	0.0	0.0	0.0010197328503878554	1.0197328503878554E-4
20	0.0	0.0	0.0	0.0016315725606205686	1.0197328503878554E-4
21	0.0	1.0197328503878554E-4	0.0	0.002243412270853282	4.0789314015514215E-4
22	0.0	1.0197328503878554E-4	0.0	0.002855251981085995	4.0789314015514215E-4
23	0.0	1.0197328503878554E-4	0.0	0.005098664251939277	4.0789314015514215E-4
24	0.0	1.0197328503878554E-4	0.0	0.009279568938529483	4.0789314015514215E-4
25	0.0	1.0197328503878554E-4	0.0	0.012134820919615479	4.0789314015514215E-4
26	0.0	1.0197328503878554E-4	0.0	0.015703885895972974	4.0789314015514215E-4
27	0.0	1.0197328503878554E-4	0.0	0.021618336428222536	5.098664251939277E-4
28	0.0	1.0197328503878554E-4	0.0	0.03609854290373008	5.098664251939277E-4
29	0.0	1.0197328503878554E-4	0.0	0.06169383744846525	5.098664251939277E-4
30	0.0	1.0197328503878554E-4	0.0	0.11237456011274166	5.098664251939277E-4
31	0.0	1.0197328503878554E-4	0.0	0.22107808196408704	5.098664251939277E-4
32	0.0	1.0197328503878554E-4	0.0	0.36129134889241715	5.098664251939277E-4
33	0.0	1.0197328503878554E-4	0.0	0.4851888902145416	5.098664251939277E-4
34	0.0	1.0197328503878554E-4	0.0	0.6378428979176035	5.098664251939277E-4
35	0.0	1.0197328503878554E-4	0.0	0.8121152420488881	5.098664251939277E-4
36	0.0	1.0197328503878554E-4	0.0	1.0591965116978654	5.098664251939277E-4
37	0.0	1.0197328503878554E-4	0.0	1.4337443876453246	5.098664251939277E-4
38	0.0	1.0197328503878554E-4	0.0	1.867232822345202	5.098664251939277E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTAGA	30	0.0057347054	29.343567	1
GCACTAT	30	0.0057347054	29.343567	1
ACGTTCA	30	0.0057447194	29.333096	11
AGACGGA	40	7.0315116E-4	27.499779	10
AGACGGT	45	0.0013980916	24.444246	6
TAGGACC	880	0.0	23.49981	4
AGGACCT	1355	0.0	22.730444	5
GGACCTG	1360	0.0	22.161587	6
ATAGGAC	110	5.2514224E-9	21.999823	3
TAAGCGC	60	2.8724E-4	21.99982	18
CTAGCAC	70	3.214756E-5	21.99982	3
ACTGTTC	635	0.0	20.787233	8
CTGTAGG	2130	0.0	20.664486	1
GACGGTG	75	5.4716264E-5	20.533167	7
GCTACGC	75	5.4716264E-5	20.533167	30
GGAAGTT	540	0.0	20.370205	29
GATATAC	725	0.0	20.338129	1
ACGCAGT	65	4.93152E-4	20.307528	38
GCACGAT	65	4.93152E-4	20.307528	23
TCTAGAC	65	4.93152E-4	20.307528	3
>>END_MODULE