FastQCFastQC Report
Thu 2 Feb 2017
SRR4062506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550403
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG18690.3395693700797416No Hit
GTATCAACGCAGAGTACTTTTTTTT16140.2932396807430192No Hit
TCCATGTACTCTGCGTTGATACCAC15670.2847004831005645No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13420.24382134545051534No Hit
TATCAACGCAGAGTACTTTTTTTTT11800.21438836634247996No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9860.17914146543532647No Hit
GAGTACATGGAAGCAGTGGTATCAA8710.1582476839697458No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7610.13826232778527733No Hit
CATGTACTCTGCGTTGATACCACTG6940.1260894290183738No Hit
GGTATCAACGCAGAGTACTTTTTTT6390.11609675092613958No Hit
GTCTTGCGCCGGTCCAAGAATTTCA5900.10719418317123998No Hit
GAATAGGACCGCGGTTCTATTTTGT5840.10610407283390534No Hit
GAATAACGCCGCCGCATCGCCAGTC5730.10410553721545848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC350.002176758816.2756672
CGGTCCA1004.0017767E-1115.20167310
GTACCGG508.736773E-515.1920041
GTGTTAA456.7795056E-414.7700041
ACGAACG602.5574593E-514.25286415
CGAACGA602.5596537E-514.25156916
AATTCCG602.56185E-514.2502745
GCCTTAT400.00529181314.2425031
GGTTCTA954.8548827E-914.00408713
CGGTTCT1009.977157E-913.30388412
TCGGAAT500.001498210213.299046519
ACAATAC500.001504380813.2917963
TAACGAA655.4251188E-513.15768713
TCACTAC655.429751E-513.1564917
GATAACG655.434387E-513.15529411
CCGATAA655.434387E-513.1552949
CGATAAC655.434387E-513.15529410
AGCCTGT604.0807677E-412.6680616
GGTTAAT905.4181874E-712.6600041
CTATTTT1301.9645086E-1012.42557417