##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062506_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	550403
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00634625901385	32.0	32.0	32.0	32.0	32.0
2	30.70558300009266	32.0	32.0	32.0	32.0	32.0
3	30.695157911566614	32.0	32.0	32.0	32.0	32.0
4	30.74202720552032	32.0	32.0	32.0	32.0	32.0
5	30.5596390281303	32.0	32.0	32.0	32.0	32.0
6	34.143850960114676	36.0	36.0	36.0	32.0	36.0
7	34.016838571010695	36.0	36.0	36.0	32.0	36.0
8	33.994594869577384	36.0	36.0	36.0	32.0	36.0
9	34.14859839063377	36.0	36.0	36.0	32.0	36.0
10	33.83983917238823	36.0	36.0	36.0	32.0	36.0
11	34.16635992173008	36.0	36.0	36.0	32.0	36.0
12	33.948392359780016	36.0	36.0	36.0	32.0	36.0
13	34.075154023506414	36.0	36.0	36.0	32.0	36.0
14	33.97455682472661	36.0	36.0	36.0	32.0	36.0
15	33.90936640970344	36.0	36.0	36.0	32.0	36.0
16	33.93359047824957	36.0	36.0	36.0	32.0	36.0
17	33.83770437297762	36.0	36.0	36.0	32.0	36.0
18	33.86426491134678	36.0	36.0	36.0	32.0	36.0
19	33.83759536194388	36.0	36.0	36.0	32.0	36.0
20	33.82278257931007	36.0	36.0	36.0	32.0	36.0
21	33.79459777653828	36.0	36.0	36.0	32.0	36.0
22	33.739441827170275	36.0	36.0	36.0	32.0	36.0
23	33.71597356845802	36.0	36.0	36.0	27.0	36.0
24	33.687946831685146	36.0	36.0	36.0	27.0	36.0
25	33.23016044607315	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	26.0
5	40.0
6	91.0
7	33.0
8	83.0
9	111.0
10	65.0
11	21.0
12	53.0
13	25.0
14	134.0
15	160.0
16	227.0
17	319.0
18	452.0
19	706.0
20	984.0
21	1379.0
22	1924.0
23	2908.0
24	3857.0
25	5413.0
26	7151.0
27	9406.0
28	12507.0
29	16684.0
30	21654.0
31	29366.0
32	41703.0
33	59025.0
34	125326.0
35	208568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85012555555152	17.46548585092504	11.159907986495964	25.524480607027478
2	16.28441492135419	20.37105340138341	37.53867789287019	25.80585378439221
3	19.352804905746083	24.418805359981828	28.248194412900297	27.98019532137179
4	12.326559240603855	15.27714179804191	36.086580464156185	36.309718497198055
5	14.1711647856351	36.99289388074078	33.72553295894444	15.110408374679679
6	33.81034348002319	35.25791447261993	17.187997011794685	13.743745035562188
7	29.685693300074906	30.70289944658168	20.98680813619472	18.624599117148698
8	28.10439450532265	32.7896725669832	20.020766883549065	19.085166044145087
9	27.451747071543274	14.259006334305091	18.75467161820308	39.53457497594856
10	15.714769056142494	26.795017317151252	32.123315579615245	25.36689804709101
11	37.356904163763666	21.22120574329794	22.231903936374767	19.189986156563627
12	24.378307672435593	23.962784875989005	29.188657173680888	22.470250277894515
13	29.70905082242168	19.63734132773531	25.632051342688435	25.02155650715458
14	23.212281407071742	20.002765099352565	25.597907256384744	31.187046237190952
15	24.968941387897054	27.550944309134596	23.10706171887363	24.37305258409472
16	25.143517171129222	25.7697904540163	24.910688300349243	24.17600407450524
17	23.356157156942288	26.036076679315578	26.140130030306448	24.46763613343569
18	24.416977908925027	24.738802100862504	27.581915990969275	23.262303999243194
19	25.033748258007705	25.613381411714194	25.799861004479148	23.55300932579895
20	24.930509066565765	24.530481415723145	26.40127921529819	24.137730302412898
21	26.02318807886057	24.65639036601362	24.7995648369321	24.52085671819371
22	24.955919361090036	25.04726499878994	25.59279133708542	24.404024303034603
23	23.701112032953436	24.88210121828741	25.9901314353085	25.426655313450652
24	23.944225717009555	25.761401925610034	25.886573855077106	24.40779850230331
25	24.373524092988056	25.164555672395032	25.824233801863688	24.63768643275322
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	116.0
2	290.0
3	464.0
4	464.0
5	464.0
6	1229.0
7	1994.0
8	1994.0
9	1994.0
10	2017.0
11	2040.0
12	2040.0
13	2040.0
14	1960.0
15	1880.0
16	1880.0
17	1880.0
18	2980.5
19	4081.0
20	4081.0
21	4081.0
22	6687.0
23	9293.0
24	9293.0
25	9293.0
26	13701.5
27	18110.0
28	18110.0
29	18110.0
30	22444.5
31	26779.0
32	26779.0
33	26779.0
34	31799.0
35	36819.0
36	36819.0
37	36819.0
38	40790.0
39	44761.0
40	44761.0
41	44761.0
42	51126.5
43	57492.0
44	57492.0
45	57492.0
46	66690.0
47	75888.0
48	75888.0
49	75888.0
50	73650.0
51	71412.0
52	71412.0
53	71412.0
54	64895.0
55	58378.0
56	58378.0
57	58378.0
58	53562.5
59	48747.0
60	48747.0
61	48747.0
62	43402.0
63	38057.0
64	38057.0
65	38057.0
66	31742.0
67	25427.0
68	25427.0
69	25427.0
70	19285.5
71	13144.0
72	13144.0
73	13144.0
74	10258.0
75	7372.0
76	7372.0
77	7372.0
78	5988.5
79	4605.0
80	4605.0
81	4605.0
82	3253.5
83	1902.0
84	1902.0
85	1902.0
86	1429.5
87	957.0
88	957.0
89	957.0
90	638.0
91	319.0
92	319.0
93	319.0
94	198.0
95	77.0
96	77.0
97	77.0
98	183.0
99	289.0
100	289.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008902567754899593
2	0.004360441349338576
3	0.005087181574228338
4	0.012899638991793286
5	0.03143151472648223
6	0.04360441349338576
7	0.06667841563363572
8	0.08884399249277347
9	0.09810993036011796
10	0.11973045205058838
11	0.12427257845614939
12	0.13335683126727144
13	0.12445426351237183
14	0.1260894290183738
15	0.11555169575747225
16	0.11682349115102934
17	0.12445426351237183
18	0.13153998070504705
19	0.13535536688571828
20	0.1255443738497065
21	0.13153998070504705
22	0.15297881733929503
23	0.14153265879728127
24	0.13789895767283245
25	0.13462862666082853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	550403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.88015836293039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.58470844030023	48.62600711321312
2	11.111650600135963	13.085114940191122
3	2.8471465129078224	5.029213126874329
4	1.0866148009198728	2.559202062306648
5	0.5371807987421068	1.5814645249730344
6	0.34780545340145363	1.2287304105461037
7	0.2320193800211489	0.9562936487239924
8	0.15787160583347976	0.743640412198833
9	0.12269626999780567	0.6501938227210496
>10	0.7747833683578014	8.845292216818319
>50	0.10757108731575288	4.525210749670149
>100	0.0837694358764153	9.077904152318709
>500	0.004636684642605993	1.713584770416834
>1k	0.001545561547535331	1.3781480490277502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	1869	0.3395693700797416	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1614	0.2932396807430192	No Hit
TCCATGTACTCTGCGTTGATACCAC	1567	0.2847004831005645	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1342	0.24382134545051534	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1180	0.21438836634247996	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	986	0.17914146543532647	No Hit
GAGTACATGGAAGCAGTGGTATCAA	871	0.1582476839697458	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	761	0.13826232778527733	No Hit
CATGTACTCTGCGTTGATACCACTG	694	0.1260894290183738	No Hit
GGTATCAACGCAGAGTACTTTTTTT	639	0.11609675092613958	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	590	0.10719418317123998	No Hit
GAATAGGACCGCGGTTCTATTTTGT	584	0.10610407283390534	No Hit
GAATAACGCCGCCGCATCGCCAGTC	573	0.10410553721545848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.8168505622244064E-4	0.0
11	0.0	0.0	0.0	1.8168505622244064E-4	0.0
12	0.0	0.0	0.0	1.8168505622244064E-4	0.0
13	0.0	0.0	0.0	1.8168505622244064E-4	1.8168505622244064E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGC	35	0.0021767588	16.275667	2
CGGTCCA	100	4.0017767E-11	15.201673	10
GTACCGG	50	8.736773E-5	15.192004	1
GTGTTAA	45	6.7795056E-4	14.770004	1
ACGAACG	60	2.5574593E-5	14.252864	15
CGAACGA	60	2.5596537E-5	14.251569	16
AATTCCG	60	2.56185E-5	14.250274	5
GCCTTAT	40	0.005291813	14.242503	1
GGTTCTA	95	4.8548827E-9	14.004087	13
CGGTTCT	100	9.977157E-9	13.303884	12
TCGGAAT	50	0.0014982102	13.2990465	19
ACAATAC	50	0.0015043808	13.291796	3
TAACGAA	65	5.4251188E-5	13.157687	13
TCACTAC	65	5.429751E-5	13.15649	17
GATAACG	65	5.434387E-5	13.155294	11
CCGATAA	65	5.434387E-5	13.155294	9
CGATAAC	65	5.434387E-5	13.155294	10
AGCCTGT	60	4.0807677E-4	12.668061	6
GGTTAAT	90	5.4181874E-7	12.660004	1
CTATTTT	130	1.9645086E-10	12.425574	17
>>END_MODULE