FastQCFastQC Report
Thu 2 Feb 2017
SRR4062506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550403
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10180.1849553872344446No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC8850.16079127475685998No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6540.11882202676947617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT300.005739839329.335941
GTATTAT454.0631036E-529.3359381
GTACTAC454.0631036E-529.3359381
TATAGAG756.998016E-826.3999442
GTATAGA651.8098202E-523.6944121
GTATAAT500.0025773422.0019531
GATATAC1950.021.4378011
TCTATCC654.92754E-420.307653
ATCACGG1152.0164589E-719.13039435
GTCCTAC2100.018.8588161
TCTATAG708.1157614E-418.8571053
GTGTTAT600.00740331118.334961
GCATTAT600.00740331118.334961
TAGACCC600.007407191718.3332944
CGTCTTA600.007407191718.33329415
TGTACCG600.007407191718.3332945
TTCGACC600.007407191718.33329429
TTCTACG750.001289677117.5999622
CTAGACA750.001289677117.5999624
CACCGGT750.001289677117.5999627
Produced by FastQC (version 0.11.5)