FastQCFastQC Report
Thu 2 Feb 2017
SRR4062505_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062505_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303392
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG87420.670711497385284No Hit
TCCATGTACTCTGCGTTGATACCAC76510.5870068252682232No Hit
GTATCAACGCAGAGTACTTTTTTTT38380.29446244874911004No Hit
GAGTACATGGAAGCAGTGGTATCAA36200.27773685890353783No Hit
CATGTACTCTGCGTTGATACCACTG32180.2468942574451892No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30650.23515565539760871No Hit
TATCAACGCAGAGTACTTTTTTTTT28420.21804645110603715No Hit
GCTTCCATGTACTCTGCGTTGATAC23700.18183324740369744No Hit
CCCATGTACTCTGCGTTGATACCAC23460.1799918980629005No Hit
GTACATGGGAAGCAGTGGTATCAAC21740.16679556112052246No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21690.16641194667452308No Hit
GCGTTGATACCACTGCTTCCATGTA19900.15267854950774595No Hit
CATGGAAGCAGTGGTATCAACGCAG19790.15183459772654737No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15940.1222962853845965No Hit
GTACATGGGGTGGTATCAACGCAAA15770.12099199626819868No Hit
GTATCAACGCAGAGTACATGGAAGC15350.11776963492180403No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14720.11293609290221207No Hit
GCGCAAGACGGACCAGAGCGAAAGC14020.107565490658221No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14010.10748876776902114No Hit
GAATAACGCCGCCGCATCGCCAGTC13890.10656809309862267No Hit
GAATAGGACCGCGGTTCTATTTTGT13870.10641464732022293No Hit
ACTCTGCGTTGATACCACTGCTTCC13370.1025785028602293No Hit
GGTATCAACGCAGAGTACTTTTTTT13350.10242505708182956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCA2800.014.59341716
CGCATCG2800.013.91251513
GCATCGC2800.013.91251514
CGAACGA1301.8189894E-1213.88854716
GATAACG1301.8189894E-1213.88427911
GCTCGTA852.6961425E-713.4124719
ACGAACG1351.8189894E-1213.37261515
GGATATA500.00150655413.2915071
AACCGCG957.378003E-813.0006847
GTTCTAA1102.7703209E-912.9462731
ATAACGA1403.6379788E-1212.894526512
CATCGCC3200.012.76776915
CGAACGT1352.7284841E-1112.66441254
TCGAACG1352.7284841E-1112.6610083
AACGTCT1457.2759576E-1212.448936
TCGCCAG3150.012.36858117
CGCCAGT3250.012.28087118
CCGACCA2400.012.2714799
CGCAAGA2500.012.1531672
CGACCAT2350.012.12829710
Produced by FastQC (version 0.11.5)