##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062505_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303392
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.128871436989026	32.0	32.0	32.0	32.0	32.0
2	30.85936617686774	32.0	32.0	32.0	32.0	32.0
3	30.857931458839705	32.0	32.0	32.0	32.0	32.0
4	30.887383841545752	32.0	32.0	32.0	32.0	32.0
5	30.732601550416145	32.0	32.0	32.0	32.0	32.0
6	34.343257439051335	36.0	36.0	36.0	32.0	36.0
7	34.21336021703371	36.0	36.0	36.0	32.0	36.0
8	34.20520227222509	36.0	36.0	36.0	32.0	36.0
9	34.34279786894503	36.0	36.0	36.0	32.0	36.0
10	34.08518772556529	36.0	36.0	36.0	32.0	36.0
11	34.36010425106184	36.0	36.0	36.0	32.0	36.0
12	34.16051962878397	36.0	36.0	36.0	32.0	36.0
13	34.274638021408755	36.0	36.0	36.0	32.0	36.0
14	34.18903062163954	36.0	36.0	36.0	32.0	36.0
15	34.145970667305	36.0	36.0	36.0	32.0	36.0
16	34.11942454764185	36.0	36.0	36.0	32.0	36.0
17	34.04441411332891	36.0	36.0	36.0	32.0	36.0
18	34.06037247428249	36.0	36.0	36.0	32.0	36.0
19	34.04937578257347	36.0	36.0	36.0	32.0	36.0
20	34.02982295426088	36.0	36.0	36.0	32.0	36.0
21	33.96827508531585	36.0	36.0	36.0	32.0	36.0
22	33.93577987282414	36.0	36.0	36.0	32.0	36.0
23	33.88905563330142	36.0	36.0	36.0	32.0	36.0
24	33.871792216002554	36.0	36.0	36.0	32.0	36.0
25	33.36262920134541	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	50.0
5	133.0
6	212.0
7	68.0
8	206.0
9	221.0
10	143.0
11	45.0
12	92.0
13	61.0
14	276.0
15	317.0
16	481.0
17	691.0
18	933.0
19	1306.0
20	1925.0
21	2560.0
22	3956.0
23	5523.0
24	7596.0
25	10788.0
26	14701.0
27	19227.0
28	26203.0
29	35137.0
30	45807.0
31	64048.0
32	93009.0
33	135635.0
34	300292.0
35	531742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15284278370291	16.93516458221438	11.302923348423233	25.60906928565948
2	16.494445126442425	20.03728823962681	36.55758961453474	26.910677019396022
3	19.083932566682932	24.370843346131302	28.07458115046665	28.470642936719116
4	12.631637911718574	15.153898569363319	34.86459442785276	37.34986909106535
5	14.459783365350889	36.54429896901678	33.44566980139393	15.550247864238397
6	34.797155969570134	34.29799992170703	16.98596636933249	13.918877739390343
7	30.232974153650083	30.38053596723237	21.030859766373258	18.35563011274429
8	28.983122301307507	31.886249271453813	19.74081801434593	19.389810412892754
9	27.441048222730437	14.341719872888712	18.380298671597938	39.83693323278292
10	15.972181682433655	26.721669344853122	31.623057802740046	25.683091169973178
11	37.494891892473184	21.09049262128107	21.339829719507286	20.07478576673846
12	24.382265543615542	23.773673034788565	28.692271289982575	23.151790131613314
13	30.078887736682415	19.202980374083037	25.083150900641392	25.634980988593153
14	23.953983573463123	19.28650879246825	24.933630154033352	31.825877480035274
15	25.718251203774038	26.373346994923757	23.150635164104223	24.75776663719798
16	26.22054066011042	25.33126876011933	23.921321941313277	24.52686863845698
17	24.14305378881453	25.534861641133418	25.744566119130578	24.57751845092147
18	25.53048309260389	23.88883170038825	26.779736014466813	23.800949192541047
19	25.05200699367312	26.160977393474006	25.237219492559237	23.549796120293635
20	25.18206712729144	23.633123504537757	26.2600673654132	24.9247420027576
21	26.36782440962484	24.626562407576834	24.054489432359496	24.951123750438832
22	25.230634713184692	24.68488694346674	24.78446305349834	25.30001528985023
23	23.84950353868895	24.478723306177265	25.225028656773297	26.4467444983605
24	24.092923923147243	26.00694471118759	24.967773159920412	24.93235820574475
25	25.17228902612432	24.386876919070442	24.620103753676815	25.820730301128414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	218.0
2	603.0
3	988.0
4	988.0
5	988.0
6	2659.0
7	4330.0
8	4330.0
9	4330.0
10	4290.5
11	4251.0
12	4251.0
13	4251.0
14	3994.5
15	3738.0
16	3738.0
17	3738.0
18	5926.5
19	8115.0
20	8115.0
21	8115.0
22	13056.0
23	17997.0
24	17997.0
25	17997.0
26	26394.0
27	34791.0
28	34791.0
29	34791.0
30	42332.0
31	49873.0
32	49873.0
33	49873.0
34	62314.5
35	74756.0
36	74756.0
37	74756.0
38	84982.0
39	95208.0
40	95208.0
41	95208.0
42	113676.0
43	132144.0
44	132144.0
45	132144.0
46	166032.0
47	199920.0
48	199920.0
49	199920.0
50	189655.0
51	179390.0
52	179390.0
53	179390.0
54	162644.5
55	145899.0
56	145899.0
57	145899.0
58	134517.0
59	123135.0
60	123135.0
61	123135.0
62	109630.0
63	96125.0
64	96125.0
65	96125.0
66	79424.0
67	62723.0
68	62723.0
69	62723.0
70	47448.5
71	32174.0
72	32174.0
73	32174.0
74	24926.5
75	17679.0
76	17679.0
77	17679.0
78	14726.0
79	11773.0
80	11773.0
81	11773.0
82	8178.0
83	4583.0
84	4583.0
85	4583.0
86	3336.0
87	2089.0
88	2089.0
89	2089.0
90	1410.0
91	731.0
92	731.0
93	731.0
94	441.5
95	152.0
96	152.0
97	152.0
98	381.0
99	610.0
100	610.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007058505806388254
2	0.0024551324543959146
3	0.00460337335199234
4	0.015037686283174978
5	0.03536925192114115
6	0.045496673295524294
7	0.06805320272028677
8	0.08754081657705433
9	0.09406226215904348
10	0.11631190002700646
11	0.11815324936780339
12	0.13042891163978296
13	0.11830669514620314
14	0.12244973116299625
15	0.11086457489381553
16	0.10956028577741769
17	0.11968770715180084
18	0.12689965873658884
19	0.1261324298445901
20	0.11792308070020377
21	0.12544192384179126
22	0.14385541724976064
23	0.13656674277577274
24	0.12904789963418525
25	0.12697638162578873
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1303392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.827984258339185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63787458060025	40.75642525872601
2	13.187943588101621	13.670090653679923
3	3.822491287767348	5.943360548701344
4	1.5500814740726678	3.2135039294952574
5	0.7866957024475596	2.0386426241277604
6	0.4529232457259753	1.4084459309833044
7	0.2854969003403058	1.03577101996494
8	0.19597659458718375	0.8125657487413984
9	0.13835284345720375	0.645349409351678
>10	0.7450269385480417	7.136534062795925
>50	0.08581432040166856	3.08304153986985
>100	0.09402540837180559	10.272530100480187
>500	0.011974926169760513	4.180943151662041
>1k	0.005026512219405648	4.546735451006589
>5k	2.956771893768028E-4	1.2560605704137349
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8742	0.670711497385284	No Hit
TCCATGTACTCTGCGTTGATACCAC	7651	0.5870068252682232	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3838	0.29446244874911004	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3620	0.27773685890353783	No Hit
CATGTACTCTGCGTTGATACCACTG	3218	0.2468942574451892	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3065	0.23515565539760871	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2842	0.21804645110603715	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2370	0.18183324740369744	No Hit
CCCATGTACTCTGCGTTGATACCAC	2346	0.1799918980629005	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2174	0.16679556112052246	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2169	0.16641194667452308	No Hit
GCGTTGATACCACTGCTTCCATGTA	1990	0.15267854950774595	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1979	0.15183459772654737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1594	0.1222962853845965	No Hit
GTACATGGGGTGGTATCAACGCAAA	1577	0.12099199626819868	No Hit
GTATCAACGCAGAGTACATGGAAGC	1535	0.11776963492180403	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1472	0.11293609290221207	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1402	0.107565490658221	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1401	0.10748876776902114	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1389	0.10656809309862267	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1387	0.10641464732022293	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1337	0.1025785028602293	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1335	0.10242505708182956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.672288919987233E-5	0.0
8	0.0	0.0	0.0	7.672288919987233E-5	0.0
9	0.0	0.0	0.0	7.672288919987233E-5	0.0
10	0.0	0.0	0.0	1.5344577839974466E-4	0.0
11	0.0	0.0	0.0	1.5344577839974466E-4	0.0
12	0.0	0.0	0.0	1.5344577839974466E-4	7.672288919987233E-5
13	0.0	0.0	0.0	2.30168667599617E-4	7.672288919987233E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCCA	280	0.0	14.593417	16
CGCATCG	280	0.0	13.912515	13
GCATCGC	280	0.0	13.912515	14
CGAACGA	130	1.8189894E-12	13.888547	16
GATAACG	130	1.8189894E-12	13.884279	11
GCTCGTA	85	2.6961425E-7	13.412471	9
ACGAACG	135	1.8189894E-12	13.372615	15
GGATATA	50	0.001506554	13.291507	1
AACCGCG	95	7.378003E-8	13.000684	7
GTTCTAA	110	2.7703209E-9	12.946273	1
ATAACGA	140	3.6379788E-12	12.8945265	12
CATCGCC	320	0.0	12.767769	15
CGAACGT	135	2.7284841E-11	12.6644125	4
TCGAACG	135	2.7284841E-11	12.661008	3
AACGTCT	145	7.2759576E-12	12.44893	6
TCGCCAG	315	0.0	12.368581	17
CGCCAGT	325	0.0	12.280871	18
CCGACCA	240	0.0	12.271479	9
CGCAAGA	250	0.0	12.153167	2
CGACCAT	235	0.0	12.128297	10
>>END_MODULE