FastQCFastQC Report
Thu 2 Feb 2017
SRR4062505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303392
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35920.2755886180059414No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31270.23991247452800077No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14940.11462399646460927No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14400.11048096044781615No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14320.10986717733421718No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14010.10748876776902114No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC13290.10196471974663034No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13120.1006604306302325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAG550.004483864619.9999492
GTATTAG3150.018.8592621
CGCAATA4100.018.7804436
TTAACGG1303.5879566E-818.61533735
TATGCGT951.5973015E-518.52626839
TATTAGA2150.017.3953042
GGTAAAC1651.6589183E-917.33328835
CGAGCCG4950.017.33328815
CGCGGTT5900.017.15249810
CGAATGC4750.017.13679743
GTCCTAT4450.016.8108791
TAGGACC7900.016.7088174
CTATTCC4750.016.6736414
ATACGAA4900.016.612240
CAATACG4650.016.55909738
TACGAAT5050.016.55441341
GTTTTCG6150.016.45524228
TAACGGC1351.0856329E-616.29625336
GACCGCG6850.016.0583527
CGCTAGA5100.015.96074237
Produced by FastQC (version 0.11.5)