##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303392
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173959177285113	32.0	32.0	32.0	32.0	32.0
2	31.34389270457391	32.0	32.0	32.0	32.0	32.0
3	31.452441015442783	32.0	32.0	32.0	32.0	32.0
4	31.580408656797033	32.0	32.0	32.0	32.0	32.0
5	31.48720645822592	32.0	32.0	32.0	32.0	32.0
6	35.07821975276816	36.0	36.0	36.0	36.0	36.0
7	35.095672675603346	36.0	36.0	36.0	36.0	36.0
8	35.03030861014952	36.0	36.0	36.0	36.0	36.0
9	35.17779225282954	36.0	36.0	36.0	36.0	36.0
10	35.03046665930127	36.0	36.0	36.0	36.0	36.0
11	35.18111205224522	36.0	36.0	36.0	36.0	36.0
12	35.07097787925659	36.0	36.0	36.0	36.0	36.0
13	35.12215971864182	36.0	36.0	36.0	36.0	36.0
14	35.07797040337826	36.0	36.0	36.0	36.0	36.0
15	35.04225743291351	36.0	36.0	36.0	36.0	36.0
16	35.04673114458275	36.0	36.0	36.0	36.0	36.0
17	35.02462958189094	36.0	36.0	36.0	36.0	36.0
18	35.02906032874223	36.0	36.0	36.0	36.0	36.0
19	34.994558045469056	36.0	36.0	36.0	36.0	36.0
20	34.988984894797575	36.0	36.0	36.0	36.0	36.0
21	34.9659879759888	36.0	36.0	36.0	36.0	36.0
22	34.94212945913432	36.0	36.0	36.0	32.0	36.0
23	34.8905049286784	36.0	36.0	36.0	32.0	36.0
24	34.86527460656502	36.0	36.0	36.0	32.0	36.0
25	34.83847376690972	36.0	36.0	36.0	32.0	36.0
26	34.78240544671135	36.0	36.0	36.0	32.0	36.0
27	34.75900880164985	36.0	36.0	36.0	32.0	36.0
28	34.727062925044805	36.0	36.0	36.0	32.0	36.0
29	34.695329570842844	36.0	36.0	36.0	32.0	36.0
30	34.67950624217426	36.0	36.0	36.0	32.0	36.0
31	34.67280910117601	36.0	36.0	36.0	32.0	36.0
32	34.616529793032335	36.0	36.0	36.0	32.0	36.0
33	34.58305789816111	36.0	36.0	36.0	32.0	36.0
34	34.55319428076895	36.0	36.0	36.0	32.0	36.0
35	34.49814330608136	36.0	36.0	36.0	32.0	36.0
36	34.45786378925143	36.0	36.0	36.0	32.0	36.0
37	34.42341674645847	36.0	36.0	36.0	32.0	36.0
38	34.36527383933613	36.0	36.0	36.0	32.0	36.0
39	34.28868828410793	36.0	36.0	36.0	32.0	36.0
40	34.2322317460902	36.0	36.0	36.0	32.0	36.0
41	34.210210742432054	36.0	36.0	36.0	32.0	36.0
42	34.102151156367384	36.0	36.0	36.0	32.0	36.0
43	34.123391888242374	36.0	36.0	36.0	32.0	36.0
44	34.09393873830743	36.0	36.0	36.0	32.0	36.0
45	33.97380910731384	36.0	36.0	36.0	32.0	36.0
46	34.03871130097469	36.0	36.0	36.0	32.0	36.0
47	33.94790285654661	36.0	36.0	36.0	32.0	36.0
48	33.93973110161793	36.0	36.0	36.0	32.0	36.0
49	33.93680335616607	36.0	36.0	36.0	32.0	36.0
50	33.36556615354398	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	27.0
22	119.0
23	457.0
24	1156.0
25	2633.0
26	5728.0
27	10792.0
28	18070.0
29	26385.0
30	35960.0
31	49862.0
32	73181.0
33	110418.0
34	232819.0
35	735781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.65585388705283	17.658460525578743	11.811441125036543	25.874244462331887
2	15.988310088034948	20.576654242234984	36.044764782337154	27.39027088739291
3	18.588194856300653	24.601478769446537	28.43458666345451	28.375739710798303
4	12.448624317068811	15.694316892347565	35.11146710613639	36.745591684447234
5	14.32723232918416	36.79975019027277	33.26973005818664	15.603287422356434
6	35.13934869589569	34.497635004238965	16.59954656528961	13.76346973457574
7	30.193142201271762	30.436660651592153	21.048771206206574	18.321425940929515
8	29.021583683189707	32.17312980285286	19.57377366133903	19.231512852618398
9	27.431348358743957	14.078266553730574	18.34275490412708	40.14763018339839
10	15.880103606589577	26.8629084726621	31.62087844639218	25.63610947435614
11	37.769220618202354	20.90261410228082	21.353744690776068	19.974420588740763
12	24.30957072009035	23.867723601188285	28.673568657779086	23.149137020942277
13	30.32395472735754	19.046227075200708	25.110864574893814	25.518953622547937
14	23.9804295254229	19.155940806756526	24.925425351697726	31.938204316122853
15	25.798761999459867	26.367738945766124	23.05998502369203	24.773514031081977
16	26.27406029805308	25.24781493211559	23.951044658859345	24.527080110971987
17	24.329288502614716	25.530462055927917	25.683524219881665	24.456725221575702
18	25.773520168913112	23.656121872775035	26.894978640347645	23.675379317964204
19	25.280422160025534	26.03836758243107	25.187280572536892	23.493929685006506
20	25.423356902604894	23.35782327956593	26.320477646019004	24.89834217181017
21	26.419526895976038	24.534138616778375	24.126663352237856	24.919671135007736
22	25.226179077361223	24.50621148510962	24.861285016326633	25.406324421202527
23	23.88207078146866	24.114387689965874	25.42865078195969	26.574890746605778
24	24.26775674547642	25.58800422282782	25.088691659915053	25.055547371780705
25	25.315944857725075	24.09321217254671	24.768757211951584	25.82208575777663
26	24.364274140089858	24.854226510520242	25.658435835113302	25.123063514276595
27	25.875791780216545	24.953122314698877	24.862666028332228	24.308419876752353
28	24.68067933515013	23.858363408705898	25.780041614495104	25.680915641648866
29	24.144156170975425	25.111555080896615	26.23493162456115	24.509357123566815
30	25.071045395399082	24.511428641575215	26.144168446637693	24.273357516388007
31	24.501991726203627	24.753719525668412	25.003912867349193	25.740375880778764
32	24.390896982642214	24.939388917532103	24.925885689032924	25.743828410792762
33	24.004060175296456	24.479511919668067	25.602044511551398	25.91438339348408
34	24.58485244653949	24.98964240995802	26.024404016596698	24.401101126905797
35	25.904332694998892	24.449436547101715	25.52348027301073	24.122750484888662
36	24.64316184233139	25.312492327711077	25.16886707912892	24.87547875082861
37	25.765464265547127	25.027313348555154	25.16740934423412	24.0398130416636
38	24.702775527239694	24.797835186958338	25.036443372369938	25.462945913432026
39	24.907472195624955	24.65259875770298	25.25241830546758	25.187510741204488
40	25.50537367115956	24.848318848051854	25.076646166310674	24.569661314477916
41	23.854603987135107	25.299065822101102	25.802598143919862	25.043732046843925
42	26.121458471434533	25.273747268665144	25.038975227713532	23.565819032186784
43	25.050483661093516	23.880075986349464	24.99424578331001	26.075194569247014
44	24.594289361911077	24.676459576244138	25.73439149542118	24.994859566423607
45	24.878547666396603	24.89619393091257	25.200093295033266	25.02516510765756
46	24.26081218975134	24.693913563860395	25.94449857678822	25.100775669600043
47	24.961638555400064	24.21297660258771	26.21521384203678	24.61017099997545
48	25.229324715818418	26.31587427266701	24.24259163781886	24.21220937369571
49	23.986797525226486	25.14554332081216	25.958192163217202	24.90946699074415
50	23.860588372492696	26.438247280940807	24.279188455967198	25.421975890599295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	20.0
2	20.0
3	111.0
4	202.0
5	222.5
6	243.0
7	229.5
8	216.0
9	322.0
10	428.0
11	667.0
12	906.0
13	1570.5
14	2235.0
15	3154.5
16	4074.0
17	4838.5
18	5603.0
19	6130.0
20	6657.0
21	7244.5
22	7832.0
23	8176.5
24	8521.0
25	8592.0
26	8663.0
27	10118.5
28	11574.0
29	13084.5
30	14595.0
31	16382.0
32	18169.0
33	20887.0
34	23605.0
35	26897.5
36	30190.0
37	36466.5
38	42743.0
39	46781.5
40	50820.0
41	56782.5
42	62745.0
43	66377.0
44	70009.0
45	81152.5
46	92296.0
47	100943.0
48	109590.0
49	109212.5
50	108835.0
51	100921.5
52	93008.0
53	90578.0
54	88148.0
55	88983.0
56	89818.0
57	87518.0
58	85218.0
59	78165.0
60	71112.0
61	63055.0
62	54998.0
63	48157.5
64	41317.0
65	35369.0
66	29421.0
67	25408.0
68	21395.0
69	19730.5
70	18066.0
71	13968.5
72	9871.0
73	9056.0
74	8241.0
75	5968.0
76	3695.0
77	3378.0
78	3061.0
79	2579.5
80	2098.0
81	1680.5
82	1263.0
83	1086.0
84	909.0
85	729.5
86	550.0
87	409.5
88	269.0
89	184.5
90	100.0
91	62.5
92	25.0
93	16.0
94	7.0
95	8.0
96	9.0
97	4.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013886842945176893
2	0.003529252903194127
3	8.439517811985957E-4
4	2.30168667599617E-4
5	0.0
6	5.370602243991063E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5344577839974466E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1303392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.0627634438584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48561279948899	44.56183107567875
2	12.760785958020385	14.308098490444134
3	3.643586696424856	6.128086171465686
4	1.4643875438877234	3.28390449852441
5	0.7441320143833589	2.085904854668804
6	0.440588773616638	1.4820374514773558
7	0.268234180128714	1.0526564571679375
8	0.18684640172702371	0.8380100496286612
9	0.1361061495593802	0.6867438179401749
>10	0.6969387274155845	7.193997810393913
>50	0.07777521809613316	3.020037181852397
>100	0.08290644028614041	9.647794435044888
>500	0.009349302200382058	3.482807422525797
>1k	0.002749794764818252	2.2280902831872047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3592	0.2755886180059414	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3127	0.23991247452800077	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1494	0.11462399646460927	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1440	0.11048096044781615	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1432	0.10986717733421718	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1401	0.10748876776902114	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1329	0.10196471974663034	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1312	0.1006604306302325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.672288919987233E-5
6	0.0	0.0	0.0	0.0	7.672288919987233E-5
7	0.0	0.0	0.0	0.0	7.672288919987233E-5
8	0.0	0.0	0.0	0.0	7.672288919987233E-5
9	0.0	0.0	0.0	0.0	7.672288919987233E-5
10	0.0	0.0	0.0	7.672288919987233E-5	7.672288919987233E-5
11	0.0	0.0	0.0	7.672288919987233E-5	7.672288919987233E-5
12	0.0	0.0	0.0	7.672288919987233E-5	1.5344577839974466E-4
13	0.0	0.0	0.0	1.5344577839974466E-4	1.5344577839974466E-4
14	0.0	0.0	0.0	1.5344577839974466E-4	1.5344577839974466E-4
15	0.0	0.0	0.0	1.5344577839974466E-4	3.0689155679948933E-4
16	0.0	0.0	0.0	2.30168667599617E-4	3.836144459993617E-4
17	0.0	0.0	0.0	3.0689155679948933E-4	3.836144459993617E-4
18	0.0	0.0	0.0	3.0689155679948933E-4	3.836144459993617E-4
19	0.0	0.0	0.0	3.836144459993617E-4	3.836144459993617E-4
20	0.0	0.0	0.0	6.137831135989787E-4	5.370602243991063E-4
21	0.0	0.0	0.0	0.0010741204487982126	5.370602243991063E-4
22	0.0	0.0	0.0	0.0019947951191966805	6.137831135989787E-4
23	0.0	0.0	0.0	0.0030689155679948935	6.137831135989787E-4
24	0.0	0.0	0.0	0.005293879354791191	6.90506002798851E-4
25	0.0	0.0	0.0	0.0064447226927892756	6.90506002798851E-4
26	0.0	0.0	0.0	0.008976578036385062	6.90506002798851E-4
27	0.0	0.0	0.0	0.01281272249637868	6.90506002798851E-4
28	0.0	0.0	0.0	0.020254842748766298	7.672288919987234E-4
29	0.0	0.0	0.0	0.030535709901549187	7.672288919987234E-4
30	0.0	0.0	0.0	0.05270862488031229	7.672288919987234E-4
31	0.0	0.0	0.0	0.09713117772703837	7.672288919987234E-4
32	0.0	0.0	0.0	0.15881638064373574	8.439517811985956E-4
33	0.0	0.0	0.0	0.2119086199700474	8.439517811985956E-4
34	0.0	0.0	0.0	0.2796549311335347	9.20674670398468E-4
35	0.0	0.0	0.0	0.34908914585941914	9.20674670398468E-4
36	0.0	0.0	0.0	0.4539693353956446	9.20674670398468E-4
37	0.0	0.0	0.0	0.6103305835849844	9.20674670398468E-4
38	0.0	0.0	0.0	0.7910897105398836	9.973975595983402E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTAAG	55	0.0044838646	19.999949	2
GTATTAG	315	0.0	18.859262	1
CGCAATA	410	0.0	18.78044	36
TTAACGG	130	3.5879566E-8	18.615337	35
TATGCGT	95	1.5973015E-5	18.526268	39
TATTAGA	215	0.0	17.395304	2
GGTAAAC	165	1.6589183E-9	17.333288	35
CGAGCCG	495	0.0	17.333288	15
CGCGGTT	590	0.0	17.152498	10
CGAATGC	475	0.0	17.136797	43
GTCCTAT	445	0.0	16.810879	1
TAGGACC	790	0.0	16.708817	4
CTATTCC	475	0.0	16.673641	4
ATACGAA	490	0.0	16.6122	40
CAATACG	465	0.0	16.559097	38
TACGAAT	505	0.0	16.554413	41
GTTTTCG	615	0.0	16.455242	28
TAACGGC	135	1.0856329E-6	16.296253	36
GACCGCG	685	0.0	16.058352	7
CGCTAGA	510	0.0	15.960742	37
>>END_MODULE