FastQCFastQC Report
Thu 2 Feb 2017
SRR4062504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700002
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG48360.6908551689852315No Hit
TCCATGTACTCTGCGTTGATACCAC42100.6014268530661341No Hit
GTATCAACGCAGAGTACTTTTTTTT21810.31157053836989035No Hit
GAGTACATGGAAGCAGTGGTATCAA20310.2901420281656338No Hit
CATGTACTCTGCGTTGATACCACTG18150.25928497347150437No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17570.2509992828591918No Hit
TATCAACGCAGAGTACTTTTTTTTT16130.23042791306310553No Hit
CCCATGTACTCTGCGTTGATACCAC13240.18914231673623788No Hit
GCTTCCATGTACTCTGCGTTGATAC12950.1849994714300816No Hit
GTACATGGGAAGCAGTGGTATCAAC11780.16828523347076152No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11740.16771380653198134No Hit
CATGGAAGCAGTGGTATCAACGCAG11440.16342810449113002No Hit
GCGTTGATACCACTGCTTCCATGTA11160.1594281159196688No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9610.13728532204193702No Hit
GGTATCAACGCAGAGTACTTTTTTT8030.11471395796012011No Hit
GTATCAACGCAGAGTACATGGAAGC8000.11428538775603497No Hit
GTACATGGGGTGGTATCAACGCAAA7500.1071425510212828No Hit
GCGCAAGACGGACCAGAGCGAAAGC7390.10557112693963733No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7370.10528541347024722No Hit
GTCTTGCGCCGGTCCAAGAATTTCA7290.10414255959268687No Hit
ACTCTGCGTTGATACCACTGCTTCC7230.10328541918451661No Hit
ACGCAGAGTACATGGAAGCAGTGGT7080.10114256816409095No Hit
GAATAGGACCGCGGTTCTATTTTGT7060.10085685469470088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAA250.00605352318.986561
GTTCTAA651.0086296E-817.5260561
TCCAACG601.4540801E-615.84365418
CGGACAT704.436224E-714.9254815
GTATTAC456.792093E-414.7673251
CCGACCA1550.014.7097889
CGGTCCA1101.2732926E-1114.68192910
CGACCAT1450.014.41390110
CTACTCA602.5744903E-514.2439954
TTAGACC400.00529003614.2439944
CGCGCTA551.9504818E-413.8242216
GACCATA1650.013.81828511
CTAGGAC707.285904E-613.5647373
CTATACT500.00150287613.29439454
TAGGACA1153.783498E-1013.211824
ACAGTCC751.475715E-512.6667618
TGCGGTA751.475715E-512.66676115
TGTACCG604.0941677E-412.6640455
TATACTG905.404272E-712.6640445
CCTATTC1750.012.4795593
Produced by FastQC (version 0.11.5)