##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062504_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700002
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.058215547955577	32.0	32.0	32.0	32.0	32.0
2	30.70781369196088	32.0	32.0	32.0	32.0	32.0
3	30.713326533352763	32.0	32.0	32.0	32.0	32.0
4	30.76612638249605	32.0	32.0	32.0	32.0	32.0
5	30.557802691992308	32.0	32.0	32.0	32.0	32.0
6	34.19268087805463	36.0	36.0	36.0	32.0	36.0
7	34.049672715220815	36.0	36.0	36.0	32.0	36.0
8	34.015097099722574	36.0	36.0	36.0	32.0	36.0
9	34.19734800757712	36.0	36.0	36.0	32.0	36.0
10	33.84227330779055	36.0	36.0	36.0	32.0	36.0
11	34.224783643475305	36.0	36.0	36.0	32.0	36.0
12	33.96574152645278	36.0	36.0	36.0	32.0	36.0
13	34.1025882783192	36.0	36.0	36.0	32.0	36.0
14	34.005875697498006	36.0	36.0	36.0	32.0	36.0
15	33.965017242807875	36.0	36.0	36.0	32.0	36.0
16	33.937214465101526	36.0	36.0	36.0	32.0	36.0
17	33.863923245933584	36.0	36.0	36.0	32.0	36.0
18	33.87741749309288	36.0	36.0	36.0	32.0	36.0
19	33.858057548407004	36.0	36.0	36.0	32.0	36.0
20	33.82938477318636	36.0	36.0	36.0	32.0	36.0
21	33.78248919288802	36.0	36.0	36.0	32.0	36.0
22	33.72910220256514	36.0	36.0	36.0	32.0	36.0
23	33.69604086845466	36.0	36.0	36.0	27.0	36.0
24	33.657265264956386	36.0	36.0	36.0	27.0	36.0
25	33.17054808414833	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	7.0
4	23.0
5	76.0
6	118.0
7	31.0
8	113.0
9	130.0
10	69.0
11	33.0
12	39.0
13	35.0
14	137.0
15	186.0
16	268.0
17	422.0
18	577.0
19	856.0
20	1293.0
21	1812.0
22	2566.0
23	3753.0
24	5025.0
25	6994.0
26	9179.0
27	11928.0
28	15867.0
29	21052.0
30	26792.0
31	36482.0
32	52204.0
33	73406.0
34	156325.0
35	272202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.19020133384386	17.24667539132373	11.290668236342958	25.272455038489454
2	15.932991318186948	20.48776445814029	37.559663356520076	26.019580867152687
3	19.27287585200932	24.84302741716348	28.27078002694428	27.613316703882916
4	12.303788352893774	15.52861768126627	35.98720927242021	36.18038469341975
5	13.946525623198431	37.20755083695732	33.86986825049983	14.976055289344426
6	33.67211741719895	35.170561659322615	17.48251648302959	13.674804440448849
7	29.342513263101917	31.041019475423052	21.32289345057949	18.293573810895545
8	28.259753630130618	33.01799515267639	20.09809038328174	18.624160833911247
9	27.3177839647315	14.447585586590423	18.907054128218657	39.327576320459414
10	15.574323841125906	27.136032724516213	32.28242058440722	25.007222849950654
11	37.00351432261682	21.346112861677007	22.07829916511595	19.572073650590227
12	24.177911802278256	24.190643494033985	29.414785642657176	22.21665906103058
13	30.018708859818005	19.760875597524937	25.422165135725056	24.798250406932006
14	23.34182980172046	20.098867995777514	25.347797354937825	31.211504847564203
15	25.060568009221782	27.573532566567604	23.65284447965859	23.713054944552027
16	25.26269119929062	25.924156720847247	25.1585729507083	23.654579129153824
17	23.33878311169471	26.28023303881644	26.579899329291056	23.801084520197794
18	24.745950368772494	24.52036688428726	28.087074938919375	22.646607808020875
19	24.70049194421676	26.47229219684063	25.79209599134842	23.035119867594194
20	24.797353255435738	24.25767711833254	27.120978711900477	23.82399091433124
21	25.794334235476548	25.115724184494965	24.482459703952074	24.60748187607642
22	24.653386864614127	25.674268070384286	25.404560842230573	24.267784222771017
23	23.13686610958273	25.296102844504343	25.885661884099648	25.681369161813283
24	23.401695511729244	26.51289945094718	25.962559118311013	24.122845919012565
25	24.423588044619535	25.229377483671065	25.55838790614906	24.78864656556033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	175.0
2	385.5
3	596.0
4	596.0
5	596.0
6	1570.0
7	2544.0
8	2544.0
9	2544.0
10	2523.5
11	2503.0
12	2503.0
13	2503.0
14	2427.5
15	2352.0
16	2352.0
17	2352.0
18	3848.0
19	5344.0
20	5344.0
21	5344.0
22	8545.0
23	11746.0
24	11746.0
25	11746.0
26	17497.0
27	23248.0
28	23248.0
29	23248.0
30	28923.0
31	34598.0
32	34598.0
33	34598.0
34	41038.0
35	47478.0
36	47478.0
37	47478.0
38	52394.5
39	57311.0
40	57311.0
41	57311.0
42	65895.5
43	74480.0
44	74480.0
45	74480.0
46	90523.0
47	106566.0
48	106566.0
49	106566.0
50	99073.5
51	91581.0
52	91581.0
53	91581.0
54	80804.5
55	70028.0
56	70028.0
57	70028.0
58	64056.0
59	58084.0
60	58084.0
61	58084.0
62	51779.0
63	45474.0
64	45474.0
65	45474.0
66	38196.0
67	30918.0
68	30918.0
69	30918.0
70	23372.5
71	15827.0
72	15827.0
73	15827.0
74	12436.5
75	9046.0
76	9046.0
77	9046.0
78	7421.5
79	5797.0
80	5797.0
81	5797.0
82	4091.5
83	2386.0
84	2386.0
85	2386.0
86	1772.5
87	1159.0
88	1159.0
89	1159.0
90	768.5
91	378.0
92	378.0
93	378.0
94	227.0
95	76.0
96	76.0
97	76.0
98	191.5
99	307.0
100	307.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00999997142865306
2	0.004714272244936443
3	0.005571412653106705
4	0.017142808163405247
5	0.03785703469418659
6	0.0489998600004
7	0.07214265102099708
8	0.09242830734769329
9	0.09728543632732477
10	0.11885680326627639
11	0.12328536204182274
12	0.13699960857254692
13	0.12399964571529795
14	0.12685678040919882
15	0.11257110693969445
16	0.11385681755194985
17	0.12728535061328397
18	0.1322853363276105
19	0.13414247387864606
20	0.12557106979694344
21	0.1322853363276105
22	0.15657098122576793
23	0.14357101836851893
24	0.13999960000114284
25	0.13371390367456093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	700002.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.94228392127543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.86613116750387	43.08161523131319
2	12.525136844208435	13.512689756062382
3	3.472657861006122	5.619692888995238
4	1.3798199643596891	2.97722561110938
5	0.6703685274411261	1.8080604719558275
6	0.43584179176987786	1.4106181005844896
7	0.2551621551706794	0.963482059412693
8	0.1860004842666678	0.8026632745445855
9	0.1483297303958625	0.7201119987881869
>10	0.8480719427806191	8.702285712532856
>50	0.10504610235643666	4.0062774080052606
>100	0.09629218427871282	10.011394665315674
>500	0.007692764033269059	2.710152432800075
>1k	0.0034484804287068195	3.673730388580207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4836	0.6908551689852315	No Hit
TCCATGTACTCTGCGTTGATACCAC	4210	0.6014268530661341	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2181	0.31157053836989035	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2031	0.2901420281656338	No Hit
CATGTACTCTGCGTTGATACCACTG	1815	0.25928497347150437	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1757	0.2509992828591918	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1613	0.23042791306310553	No Hit
CCCATGTACTCTGCGTTGATACCAC	1324	0.18914231673623788	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1295	0.1849994714300816	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1178	0.16828523347076152	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1174	0.16771380653198134	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1144	0.16342810449113002	No Hit
GCGTTGATACCACTGCTTCCATGTA	1116	0.1594281159196688	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	961	0.13728532204193702	No Hit
GGTATCAACGCAGAGTACTTTTTTT	803	0.11471395796012011	No Hit
GTATCAACGCAGAGTACATGGAAGC	800	0.11428538775603497	No Hit
GTACATGGGGTGGTATCAACGCAAA	750	0.1071425510212828	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	739	0.10557112693963733	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	737	0.10528541347024722	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	729	0.10414255959268687	No Hit
ACTCTGCGTTGATACCACTGCTTCC	723	0.10328541918451661	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	708	0.10114256816409095	No Hit
GAATAGGACCGCGGTTCTATTTTGT	706	0.10085685469470088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.4285673469504372E-4	0.0
10	0.0	0.0	0.0	1.4285673469504372E-4	0.0
11	0.0	0.0	0.0	2.8571346939008745E-4	0.0
12	0.0	0.0	0.0	4.285702040851312E-4	0.0
13	0.0	0.0	0.0	4.285702040851312E-4	1.4285673469504372E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCTAA	25	0.006053523	18.98656	1
GTTCTAA	65	1.0086296E-8	17.526056	1
TCCAACG	60	1.4540801E-6	15.843654	18
CGGACAT	70	4.436224E-7	14.925481	5
GTATTAC	45	6.792093E-4	14.767325	1
CCGACCA	155	0.0	14.709788	9
CGGTCCA	110	1.2732926E-11	14.681929	10
CGACCAT	145	0.0	14.413901	10
CTACTCA	60	2.5744903E-5	14.243995	4
TTAGACC	40	0.005290036	14.243994	4
CGCGCTA	55	1.9504818E-4	13.82422	16
GACCATA	165	0.0	13.818285	11
CTAGGAC	70	7.285904E-6	13.564737	3
CTATACT	50	0.001502876	13.2943945	4
TAGGACA	115	3.783498E-10	13.21182	4
ACAGTCC	75	1.475715E-5	12.666761	8
TGCGGTA	75	1.475715E-5	12.666761	15
TGTACCG	60	4.0941677E-4	12.664045	5
TATACTG	90	5.404272E-7	12.664044	5
CCTATTC	175	0.0	12.479559	3
>>END_MODULE