FastQCFastQC Report
Thu 2 Feb 2017
SRR4062504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700002
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20270.28957060122685363No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18430.2632849620429656No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8520.12171393796017727No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8340.11914251673566646No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7670.10957111551109854No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7550.107856834694758No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7290.10414255959268687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7180.10257113551104141No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7160.10228542204165131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT300.00574356429.33314343
TCCTATA858.223651E-925.8821832
GTCCTAC3650.022.3075981
TAGTACG703.2130207E-521.9998592
GAAGTAT602.8712305E-421.9998576
ATCACGG1551.8189894E-1221.29018435
GTCCTAT2400.021.0892241
GCATAAG654.920037E-420.3133641
TCGCAAT550.00447452120.0055851
TCACGGA1653.6379788E-1219.9998736
AATCACG1653.6379788E-1219.9998734
ATAATAC550.00448192819.9998683
AATACGA2200.019.99986839
CTATTCC2000.019.7998714
TCTAGAC808.982136E-519.2498743
CCAGTAC808.982136E-519.2498743
TGCGTAG808.982136E-519.24987413
CAATACG2300.019.13031238
TCCTACA4300.018.9301092
CTAGACA1303.5810444E-818.6152634
Produced by FastQC (version 0.11.5)