##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700002
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197565149813858	32.0	32.0	32.0	32.0	32.0
2	31.399168859517545	32.0	32.0	32.0	32.0	32.0
3	31.49780286342039	32.0	32.0	32.0	32.0	32.0
4	31.608701117996805	32.0	32.0	32.0	32.0	32.0
5	31.538168462375822	32.0	32.0	32.0	32.0	32.0
6	35.12840963311533	36.0	36.0	36.0	36.0	36.0
7	35.14120102513993	36.0	36.0	36.0	36.0	36.0
8	35.081545481298626	36.0	36.0	36.0	36.0	36.0
9	35.206172268079236	36.0	36.0	36.0	36.0	36.0
10	35.09319401944566	36.0	36.0	36.0	36.0	36.0
11	35.22787934891615	36.0	36.0	36.0	36.0	36.0
12	35.12872106079697	36.0	36.0	36.0	36.0	36.0
13	35.15529241345025	36.0	36.0	36.0	36.0	36.0
14	35.125945354441846	36.0	36.0	36.0	36.0	36.0
15	35.095168299519145	36.0	36.0	36.0	36.0	36.0
16	35.09923828789061	36.0	36.0	36.0	36.0	36.0
17	35.07366836094754	36.0	36.0	36.0	36.0	36.0
18	35.08052691278025	36.0	36.0	36.0	36.0	36.0
19	35.05826697638007	36.0	36.0	36.0	36.0	36.0
20	35.03734417901663	36.0	36.0	36.0	36.0	36.0
21	35.01384710329399	36.0	36.0	36.0	36.0	36.0
22	34.980741483595764	36.0	36.0	36.0	36.0	36.0
23	34.94390301741995	36.0	36.0	36.0	32.0	36.0
24	34.91055454127274	36.0	36.0	36.0	32.0	36.0
25	34.88636889608887	36.0	36.0	36.0	32.0	36.0
26	34.83282333479047	36.0	36.0	36.0	32.0	36.0
27	34.80124342501878	36.0	36.0	36.0	32.0	36.0
28	34.772286364896104	36.0	36.0	36.0	32.0	36.0
29	34.73032505621413	36.0	36.0	36.0	32.0	36.0
30	34.71151225282213	36.0	36.0	36.0	32.0	36.0
31	34.694395158870975	36.0	36.0	36.0	32.0	36.0
32	34.65104385416042	36.0	36.0	36.0	32.0	36.0
33	34.61910823111934	36.0	36.0	36.0	32.0	36.0
34	34.583771189225175	36.0	36.0	36.0	32.0	36.0
35	34.51489281459196	36.0	36.0	36.0	32.0	36.0
36	34.490797169150945	36.0	36.0	36.0	32.0	36.0
37	34.441253024991354	36.0	36.0	36.0	32.0	36.0
38	34.39126173925217	36.0	36.0	36.0	32.0	36.0
39	34.296707723692215	36.0	36.0	36.0	32.0	36.0
40	34.24434930185914	36.0	36.0	36.0	32.0	36.0
41	34.22344079016917	36.0	36.0	36.0	32.0	36.0
42	34.11568824089074	36.0	36.0	36.0	32.0	36.0
43	34.138931031625624	36.0	36.0	36.0	32.0	36.0
44	34.093828303347706	36.0	36.0	36.0	32.0	36.0
45	33.976070068371236	36.0	36.0	36.0	32.0	36.0
46	34.026388496032865	36.0	36.0	36.0	32.0	36.0
47	33.942903020277086	36.0	36.0	36.0	32.0	36.0
48	33.92161879537487	36.0	36.0	36.0	32.0	36.0
49	33.925383070334085	36.0	36.0	36.0	32.0	36.0
50	33.36362896106011	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	2.0
21	22.0
22	76.0
23	205.0
24	572.0
25	1425.0
26	3078.0
27	5816.0
28	9608.0
29	13909.0
30	19103.0
31	26273.0
32	38125.0
33	56391.0
34	118722.0
35	406672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60486854193784	17.82631618803768	11.767571915797113	25.80124335422736
2	15.403956707087099	20.848000502874385	37.37056699086826	26.37747579917026
3	18.66524951571152	24.966856953468305	28.802021725838433	27.565871804981747
4	12.0362685196164	16.036834233093952	36.0952341496655	35.831663097624144
5	13.607389693172307	37.49689286602038	33.78947488721461	15.106242553592706
6	33.8862396749705	35.393386838248105	17.201334860956745	13.519038625824646
7	29.37620178228062	30.942768734946473	21.3005105699698	18.380518912803108
8	28.23606218267948	33.062048394147446	20.07208550832712	18.629803914845958
9	27.25606498267148	14.222102222565077	18.806660552398423	39.71517224236502
10	15.550669855228985	27.094208302261997	32.35276470638656	25.002357136122466
11	37.1764652100994	21.18408233119334	22.12079394058874	19.51865851811852
12	24.092502592849733	24.054359844686157	29.468630089628316	22.38450747283579
13	30.271199225145075	19.683372333221904	25.401213139391032	24.644215302241992
14	23.39136173896646	19.853371847509006	25.478784346330443	31.276482067194095
15	25.124785357756117	27.563635532469906	23.5459327259065	23.765646383867477
16	25.469070088371176	25.70935511612824	25.159213830817627	23.662360964682957
17	23.36750466427239	26.178210919397372	26.604781129196773	23.849503287133466
18	24.82878620346799	24.367501807137693	28.178490918597376	22.62522107079694
19	24.8833574761215	26.224353644703875	25.8229262202108	23.069362658963833
20	24.93078591204025	24.083788332033336	27.260064971242937	23.72536078468347
21	25.88278319204802	24.85350041857023	24.664500958568688	24.599215430813054
22	24.586644038159893	25.57321264796386	25.52049851286139	24.319644801014853
23	23.15821954794415	24.914643101019713	26.089639743886444	25.837497607149697
24	23.665360956111552	26.177210922254506	26.010211399396	24.147216722237935
25	24.62935820183371	24.833500475712928	25.631355338984747	24.90578598346862
26	23.233790760597827	25.746926437353036	26.596209725115074	24.423073076934067
27	24.924500215713667	25.64735529327059	25.822211936537325	23.605932554478414
28	23.68450375856069	24.774500644283872	26.428067348379003	25.112928248776434
29	23.283933474475788	25.756926408781688	26.845637583892618	24.113502532849907
30	24.282502049994143	25.28821346224725	26.788780603483993	23.640503884274615
31	23.894931728766487	25.294927728777918	25.70235513612818	25.10778540632741
32	23.509932828763347	25.787783463475815	25.920640226742208	24.781643481018627
33	23.284504901414568	25.126499638572465	26.441638738175033	25.147356721837937
34	23.580932625906783	25.880497484292903	27.058779832057624	23.47979005774269
35	25.018357090408312	25.327927634492475	26.40721026511353	23.246505009985686
36	23.701075139785317	26.055925554498415	26.20849654715272	24.034502758563548
37	24.924928785917754	25.799497715720815	25.781640623883934	23.4939328744775
38	23.911788823460505	25.665069528372776	25.690926597352583	24.73221505081414
39	24.231787909177402	25.326927637349606	26.041068454090134	24.40021599938286
40	25.058071262653534	25.337784749186433	25.816497667149523	23.78764632101051
41	23.311361967537238	25.793783446333013	26.77249493572876	24.122359650401
42	25.423641646738155	26.13592532592764	25.907783120619655	22.532649906714553
43	24.092074022645647	24.684215187956607	26.092782592049733	25.130928197348005
44	23.597646863866103	25.58021262796392	26.561781252053567	24.260359256116413
45	24.08393118876803	25.760212113679675	25.797640578169776	24.35821611938252
46	23.39479030059914	25.249213573675505	26.58735260756398	24.768643518161376
47	24.254930700198	24.920785940611598	26.882923191648022	23.941360167542378
48	24.24935928754489	26.823780503484272	25.079785486327182	23.84707472264365
49	23.370076085496898	25.629783914903097	26.783494904300277	24.216645095299725
50	23.218076519781373	27.25577926920209	24.749643572446935	24.776500638569605
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	15.0
2	14.0
3	64.5
4	115.0
5	131.5
6	148.0
7	160.0
8	172.0
9	222.0
10	272.0
11	374.5
12	477.0
13	864.0
14	1251.0
15	1828.5
16	2406.0
17	2769.0
18	3132.0
19	3554.0
20	3976.0
21	4342.5
22	4709.0
23	5002.5
24	5296.0
25	5510.0
26	5724.0
27	6867.0
28	8010.0
29	9527.5
30	11045.0
31	12547.5
32	14050.0
33	16407.5
34	18765.0
35	21371.5
36	23978.0
37	26820.0
38	29662.0
39	31191.5
40	32721.0
41	34886.5
42	37052.0
43	38120.5
44	39189.0
45	44187.5
46	49186.0
47	52957.0
48	56728.0
49	56124.0
50	55520.0
51	50746.5
52	45973.0
53	43663.0
54	41353.0
55	41776.0
56	42199.0
57	41033.0
58	39867.0
59	36366.0
60	32865.0
61	29117.5
62	25370.0
63	22184.5
64	18999.0
65	16590.0
66	14181.0
67	12547.5
68	10914.0
69	10041.0
70	9168.0
71	7145.5
72	5123.0
73	4610.0
74	4097.0
75	3031.0
76	1965.0
77	1774.5
78	1584.0
79	1346.0
80	1108.0
81	894.0
82	680.0
83	563.0
84	446.0
85	376.0
86	306.0
87	214.0
88	122.0
89	84.0
90	46.0
91	28.5
92	11.0
93	8.0
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01728566489810029
2	0.003714275102071137
3	8.571404081702625E-4
4	1.4285673469504372E-4
5	0.0
6	5.714269387801749E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	700002.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.93865225918981
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62145724459798	47.51720033168498
2	12.311517902028022	14.512485448208393
3	3.29349882387549	5.823431456893443
4	1.2624539242941861	2.976293313488983
5	0.6650530007298691	1.9598663771974227
6	0.4050501015656383	1.4323864250235998
7	0.24821432252327066	1.02405923486646
8	0.15657811544372863	0.7382802478029759
9	0.1332402470663731	0.7067700529896148
>10	0.7312115661176503	8.212821470709358
>50	0.08574069481081356	3.6258416653374717
>100	0.08086885809189416	9.171684879157697
>500	0.004628037059357312	1.7432891319861445
>1k	4.8716179572182237E-4	0.5555899646534118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2027	0.28957060122685363	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1843	0.2632849620429656	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	852	0.12171393796017727	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	834	0.11914251673566646	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	767	0.10957111551109854	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	755	0.107856834694758	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	729	0.10414255959268687	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	718	0.10257113551104141	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	716	0.10228542204165131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.4285673469504372E-4	0.0
12	0.0	0.0	0.0	2.8571346939008745E-4	2.8571346939008745E-4
13	0.0	0.0	0.0	2.8571346939008745E-4	2.8571346939008745E-4
14	0.0	0.0	0.0	4.285702040851312E-4	2.8571346939008745E-4
15	0.0	0.0	0.0	4.285702040851312E-4	2.8571346939008745E-4
16	0.0	0.0	0.0	4.285702040851312E-4	2.8571346939008745E-4
17	0.0	0.0	0.0	5.714269387801749E-4	2.8571346939008745E-4
18	0.0	0.0	0.0	9.99997142865306E-4	2.8571346939008745E-4
19	0.0	0.0	0.0	9.99997142865306E-4	2.8571346939008745E-4
20	0.0	0.0	0.0	0.0017142808163405248	2.8571346939008745E-4
21	0.0	0.0	0.0	0.0018571375510355685	2.8571346939008745E-4
22	0.0	0.0	0.0	0.003285704897986006	2.8571346939008745E-4
23	0.0	0.0	0.0	0.005285699183716618	2.8571346939008745E-4
24	0.0	0.0	0.0	0.008142833877617493	2.8571346939008745E-4
25	0.0	0.0	0.0	0.00999997142865306	2.8571346939008745E-4
26	0.0	0.0	0.0	0.013714246530724198	2.8571346939008745E-4
27	0.0	0.0	0.0	0.018285662040965597	2.8571346939008745E-4
28	0.0	0.0	0.0	0.025857068979802916	2.8571346939008745E-4
29	0.0	0.0	0.0	0.03871417510235685	2.8571346939008745E-4
30	0.0	0.0	0.0	0.0637141036739895	2.8571346939008745E-4
31	0.0	0.0	0.0	0.1149996714295102	2.8571346939008745E-4
32	0.0	0.0	0.0	0.1708566546952723	2.8571346939008745E-4
33	0.0	0.0	0.0	0.22914220245085015	2.8571346939008745E-4
34	0.0	0.0	0.0	0.2985705755126414	2.8571346939008745E-4
35	0.0	0.0	0.0	0.3742846449010146	2.8571346939008745E-4
36	0.0	0.0	0.0	0.4787129179630915	2.8571346939008745E-4
37	0.0	0.0	0.0	0.6462838677603778	2.8571346939008745E-4
38	0.0	0.0	0.0	0.8337119036802751	2.8571346939008745E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGT	30	0.005743564	29.333143	43
TCCTATA	85	8.223651E-9	25.882183	2
GTCCTAC	365	0.0	22.307598	1
TAGTACG	70	3.2130207E-5	21.999859	2
GAAGTAT	60	2.8712305E-4	21.999857	6
ATCACGG	155	1.8189894E-12	21.290184	35
GTCCTAT	240	0.0	21.089224	1
GCATAAG	65	4.920037E-4	20.313364	1
TCGCAAT	55	0.004474521	20.005585	1
TCACGGA	165	3.6379788E-12	19.99987	36
AATCACG	165	3.6379788E-12	19.99987	34
ATAATAC	55	0.004481928	19.999868	3
AATACGA	220	0.0	19.999868	39
CTATTCC	200	0.0	19.799871	4
TCTAGAC	80	8.982136E-5	19.249874	3
CCAGTAC	80	8.982136E-5	19.249874	3
TGCGTAG	80	8.982136E-5	19.249874	13
CAATACG	230	0.0	19.130312	38
TCCTACA	430	0.0	18.930109	2
CTAGACA	130	3.5810444E-8	18.615263	4
>>END_MODULE