FastQCFastQC Report
Thu 2 Feb 2017
SRR4062503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696510
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33980.2002935438046342No Hit
GCGCAAGACGGACCAGAGCGAAAGC21990.12961904144390543No Hit
TATCAACGCAGAGTACTTTTTTTTT21090.12431403292641953No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18550.10934211999929268No Hit
GAATAACGCCGCCGCATCGCCAGTC18040.10633594850605066No Hit
GAATAGGACCGCGGTTCTATTTTGT17830.10509811318530395No Hit
GAACTACGACGGTATCTGATCGTCT17470.1029761097783096No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17200.10138460722306382No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17190.10132566268398063No Hit
GATTAAGAGGGACGGCCGGGGGCAT17150.10108988452764793No Hit
GTATCTGATCGTCTTCGAACCTCCG17090.10073621729314887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG500.001500715713.298745
AATCGTA655.4488446E-513.15492913
AAGACGG4400.012.521545
CGGACCA4500.012.4562149
TCGCCAG3600.012.4056317
CGCAAGA4600.012.3868412
CGCCAGT3850.011.8468818
GCTAGTC1052.721481E-711.7614847
CGCATCG3800.011.75096713
CGACCAT3400.011.73591310
TTATACA1800.011.6089834
CAAGACG4750.011.5978744
CGAACGA1657.2759576E-1211.5174616
GCTCGTA1251.8302671E-811.4006029
GTAGCAC1001.9353593E-611.3972393
TCTTATA1354.7548383E-911.2552032
TAGAGTG2450.011.2438325
ATCGCCA4000.011.16473816
CCGACCA3500.011.129169
GCGCAAG5050.011.0930481
Produced by FastQC (version 0.11.5)