##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062503_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696510
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173718398358982	32.0	32.0	32.0	32.0	32.0
2	30.90558499507813	32.0	32.0	32.0	32.0	32.0
3	30.88380027232377	32.0	32.0	32.0	32.0	32.0
4	30.953487453654855	32.0	32.0	32.0	32.0	32.0
5	30.78116957754449	32.0	32.0	32.0	32.0	32.0
6	34.39897200723839	36.0	36.0	36.0	32.0	36.0
7	34.29822635881899	36.0	36.0	36.0	32.0	36.0
8	34.298954913322056	36.0	36.0	36.0	32.0	36.0
9	34.43040889826762	36.0	36.0	36.0	32.0	36.0
10	34.15912373048199	36.0	36.0	36.0	32.0	36.0
11	34.40597343959069	36.0	36.0	36.0	32.0	36.0
12	34.22015726403028	36.0	36.0	36.0	32.0	36.0
13	34.32807705230149	36.0	36.0	36.0	32.0	36.0
14	34.253591785489036	36.0	36.0	36.0	32.0	36.0
15	34.19551962558429	36.0	36.0	36.0	32.0	36.0
16	34.196113197092856	36.0	36.0	36.0	32.0	36.0
17	34.130937630783194	36.0	36.0	36.0	32.0	36.0
18	34.14718922965382	36.0	36.0	36.0	32.0	36.0
19	34.12616783868059	36.0	36.0	36.0	32.0	36.0
20	34.11881745465691	36.0	36.0	36.0	32.0	36.0
21	34.08293496649003	36.0	36.0	36.0	32.0	36.0
22	34.051869426057024	36.0	36.0	36.0	32.0	36.0
23	34.02594443887746	36.0	36.0	36.0	32.0	36.0
24	33.9908447341896	36.0	36.0	36.0	32.0	36.0
25	33.549927203494235	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	60.0
5	153.0
6	277.0
7	63.0
8	253.0
9	308.0
10	183.0
11	64.0
12	135.0
13	101.0
14	207.0
15	266.0
16	464.0
17	660.0
18	915.0
19	1351.0
20	2121.0
21	3075.0
22	4566.0
23	6765.0
24	9364.0
25	13062.0
26	18245.0
27	23994.0
28	32963.0
29	44438.0
30	58331.0
31	80406.0
32	116225.0
33	170239.0
34	387402.0
35	719841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13004678866579	17.800502372445326	10.969604757505964	26.09984608138292
2	17.0590236003624	20.38047530824536	36.36797160199168	26.192529489400556
3	19.13493681735361	24.065346601188022	28.442064546097072	28.357652035361298
4	12.755236790412836	15.321458637432292	35.95440107341529	35.968903498739586
5	14.829451111362694	36.195941356277594	33.50297416883656	15.471633363523152
6	33.789943028461	35.47721108954751	16.96597216939435	13.766873712597134
7	29.784016335910103	30.61172368152525	21.067284178538944	18.5369758040257
8	27.885808341371465	32.465804742646945	20.03752112775767	19.61086578822391
9	27.268318383784983	14.07957765633011	18.990951211352964	39.66115274853194
10	16.03124610884207	26.50997582244694	31.586907887476713	25.871870181234275
11	37.42480233503724	21.360916924653033	22.160428766846817	19.05385197346291
12	24.417621190095502	23.93500328454035	28.804149911969596	22.843225613394548
13	29.125794188013206	19.598665799546534	25.518590166527193	25.75694984591307
14	23.252396501753665	19.941703763288242	25.062955568447325	31.742944166510767
15	25.01240680146462	27.37480342374612	22.416036491633893	25.19675328315537
16	25.571544737516067	25.838566567804662	23.978383328868215	24.611505365811055
17	23.739586554645655	25.929508440526806	25.425626341147893	24.90527866367965
18	24.568005429810842	24.923953138371647	26.555139138902824	23.952902292914686
19	25.168195411816853	25.11767334057719	25.64225297746751	24.07187827013845
20	25.443607141149897	24.501950767825285	25.121380781263188	24.933061309761626
21	26.628161861828897	24.320352421659482	24.534005691911982	24.517480024599635
22	25.564896459999893	24.697532383737027	25.014182550828433	24.72338860543465
23	24.3013277517469	24.422746145246386	25.644308446399418	25.631617656607297
24	24.52367025781803	25.213766270406442	25.437699239253547	24.824864232521985
25	24.562902857399177	24.73518609127175	25.52866872100209	25.173242330326985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	163.0
1	163.0
2	490.0
3	817.0
4	817.0
5	817.0
6	2093.0
7	3369.0
8	3369.0
9	3369.0
10	3756.0
11	4143.0
12	4143.0
13	4143.0
14	4816.0
15	5489.0
16	5489.0
17	5489.0
18	8967.5
19	12446.0
20	12446.0
21	12446.0
22	19601.0
23	26756.0
24	26756.0
25	26756.0
26	39504.5
27	52253.0
28	52253.0
29	52253.0
30	65720.0
31	79187.0
32	79187.0
33	79187.0
34	95409.5
35	111632.0
36	111632.0
37	111632.0
38	125968.0
39	140304.0
40	140304.0
41	140304.0
42	158680.0
43	177056.0
44	177056.0
45	177056.0
46	196818.5
47	216581.0
48	216581.0
49	216581.0
50	218985.5
51	221390.0
52	221390.0
53	221390.0
54	204122.0
55	186854.0
56	186854.0
57	186854.0
58	172096.5
59	157339.0
60	157339.0
61	157339.0
62	139933.0
63	122527.0
64	122527.0
65	122527.0
66	102270.5
67	82014.0
68	82014.0
69	82014.0
70	62356.5
71	42699.0
72	42699.0
73	42699.0
74	33788.5
75	24878.0
76	24878.0
77	24878.0
78	20626.5
79	16375.0
80	16375.0
81	16375.0
82	11658.0
83	6941.0
84	6941.0
85	6941.0
86	5095.0
87	3249.0
88	3249.0
89	3249.0
90	2209.0
91	1169.0
92	1169.0
93	1169.0
94	700.5
95	232.0
96	232.0
97	232.0
98	439.5
99	647.0
100	647.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009372181714225086
2	0.0031240605714083617
3	0.004656618587570955
4	0.014264578458128747
5	0.033834165433743395
6	0.04426734885146565
7	0.065074771147827
8	0.08635374975685378
9	0.09354498352500132
10	0.11570813022027575
11	0.11647440922835704
12	0.13020848683473718
13	0.1185964126353514
14	0.12148469505042704
15	0.11052101078095619
16	0.11152306794537022
17	0.12177941774584294
18	0.12732020441966152
19	0.12973693052207178
20	0.12077736058142892
21	0.1282633170449924
22	0.14895285026318736
23	0.13958066854896228
24	0.13280204655439698
25	0.13009059775657084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1696510.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.03654843918891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60234316882132	44.36074764122962
2	12.24365198737526	13.476966913515003
3	3.292923961151039	5.43693507283365
4	1.2999881758499847	2.8618744884216207
5	0.6653522152648847	1.8309344712273738
6	0.3905777843193008	1.2897631887576164
7	0.24822665323353402	0.9563076757209601
8	0.18571299732225816	0.8176801898330737
9	0.14157317459249105	0.7012528993034445
>10	0.7502273104960003	7.655849883482303
>50	0.07752376980680213	3.027707650330218
>100	0.08618927520333412	10.145712019755175
>500	0.011728345174389367	4.249203663023296
>1k	0.00398118138947162	3.1890642425666935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3398	0.2002935438046342	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2199	0.12961904144390543	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2109	0.12431403292641953	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1855	0.10934211999929268	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1804	0.10633594850605066	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1783	0.10509811318530395	No Hit
GAACTACGACGGTATCTGATCGTCT	1747	0.1029761097783096	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1720	0.10138460722306382	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1719	0.10132566268398063	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1715	0.10108988452764793	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1709	0.10073621729314887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1788907816635328E-4
2	0.0	0.0	0.0	0.0	1.1788907816635328E-4
3	0.0	0.0	0.0	0.0	1.1788907816635328E-4
4	0.0	0.0	0.0	5.894453908317664E-5	1.1788907816635328E-4
5	0.0	0.0	0.0	5.894453908317664E-5	1.1788907816635328E-4
6	0.0	0.0	0.0	5.894453908317664E-5	1.1788907816635328E-4
7	0.0	0.0	0.0	5.894453908317664E-5	1.1788907816635328E-4
8	0.0	0.0	0.0	1.1788907816635328E-4	1.1788907816635328E-4
9	0.0	0.0	0.0	1.1788907816635328E-4	1.1788907816635328E-4
10	0.0	0.0	0.0	1.1788907816635328E-4	1.1788907816635328E-4
11	0.0	0.0	0.0	1.1788907816635328E-4	1.1788907816635328E-4
12	0.0	0.0	0.0	1.1788907816635328E-4	2.3577815633270656E-4
13	0.0	0.0	0.0	1.1788907816635328E-4	2.947226954158832E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAACCG	50	0.0015007157	13.29874	5
AATCGTA	65	5.4488446E-5	13.154929	13
AAGACGG	440	0.0	12.52154	5
CGGACCA	450	0.0	12.456214	9
TCGCCAG	360	0.0	12.40563	17
CGCAAGA	460	0.0	12.386841	2
CGCCAGT	385	0.0	11.84688	18
GCTAGTC	105	2.721481E-7	11.761484	7
CGCATCG	380	0.0	11.750967	13
CGACCAT	340	0.0	11.735913	10
TTATACA	180	0.0	11.608983	4
CAAGACG	475	0.0	11.597874	4
CGAACGA	165	7.2759576E-12	11.51746	16
GCTCGTA	125	1.8302671E-8	11.400602	9
GTAGCAC	100	1.9353593E-6	11.397239	3
TCTTATA	135	4.7548383E-9	11.255203	2
TAGAGTG	245	0.0	11.243832	5
ATCGCCA	400	0.0	11.164738	16
CCGACCA	350	0.0	11.12916	9
GCGCAAG	505	0.0	11.093048	1
>>END_MODULE