FastQCFastQC Report
Thu 2 Feb 2017
SRR4062503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696510
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19080.11246618057070103No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18450.10875267460846089No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17740.10456761233355535No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17600.10374238878639089No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17260.10173827445756288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.020.1667211
GATATAC3000.019.8027881
GTCCTAT6250.017.9545271
ATACCGT4250.017.60046
ATCGTTT6950.017.40995629
AACGCAG38550.016.7215216
ACCGTCG4500.016.622118
TAGGACG4900.016.6126234
CGTTTAT7300.016.57523231
CCGTCGT4650.016.559039
TAGACCG800.00198849316.5003765
TACCGTC4300.016.3719847
GCACCGT1753.6270649E-916.343236
CGTCGTA4450.016.31449710
GTCCTAC5950.016.2711961
ATTAGAC953.346729E-416.2108963
GCATCGT7650.016.10446727
CGCAATA6450.016.03090136
ATAACGC7750.015.8971373
CGGCATC7900.015.87331125
Produced by FastQC (version 0.11.5)