FastQCFastQC Report
Thu 2 Feb 2017
SRR4062502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879221
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34870.1855556105428792No Hit
TATCAACGCAGAGTACTTTTTTTTT23110.1229764886620573No Hit
GCGCAAGACGGACCAGAGCGAAAGC22460.11951760862612754No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20700.11015202575960997No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20460.10887490082326667No Hit
GAATAGGACCGCGGTTCTATTTTGT19850.1056288749433941No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19530.10392604169493637No Hit
GAACTACGACGGTATCTGATCGTCT19420.10334069276577902No Hit
GAATAACGCCGCCGCATCGCCAGTC19210.10222320844647861No Hit
GATTAAGAGGGACGGCCGGGGGCAT18930.1007332293540781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA907.2759576E-1216.89336616
ACGAACG1006.5847416E-1014.25187815
GACAACG500.001498862313.3010447
CGTCGTA3150.013.270884510
ACTGCGC655.4482858E-513.155238
CGCCAGT3850.012.83491318
AAGACGG5150.012.7261045
ATCGCCA3950.012.509644516
CGCATCG4000.012.3509713
CAAGACG5000.012.347354
GTCGTAG3500.012.21426911
CATCGCC4300.011.93180615
ATAACGA1201.0000804E-811.87624912
TAACGAA1201.0006261E-811.87593213
ACCGTCG3450.011.84182558
CGCGTAA1701.8189894E-1211.73621610
CCGTCGT3600.011.6123329
CGCAAGA5050.011.4700362
ATACCGT3900.011.4490086
TGTAGGA3650.011.4468542
Produced by FastQC (version 0.11.5)