FastQCFastQC Report
Thu 2 Feb 2017
SRR4062502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879221
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20930.11137593715693896No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20630.10977953098650983No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20210.10754456234790906No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19240.10238284906352152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3450.019.768014
GTATCAA34550.018.8514561
TATCGAG600.007413825518.33323510
CGCAATA5850.017.67511636
GTCGATA1154.275653E-617.21729936
ATACGAA6100.016.95072740
CGTCGTA6500.016.92298310
AACGCAG41050.015.8634966
CGAATGC6400.015.81241543
TAGCGGC6750.015.64435930
CGGTTAA850.002980870715.52932844
CCGTCGT7100.015.4928749
CTAGCGG6550.015.45029829
TACGAAT6700.015.43275341
CAATACG6750.015.31843538
TCTAGCG6650.015.21796128
ACCGTCG7250.015.1723328
TCGATAT1452.2872246E-615.17233137
ATACCGT7850.015.1336776
GAGCGTT1756.415212E-815.0856327
Produced by FastQC (version 0.11.5)