##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1879221
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317726866611217	32.0	32.0	32.0	32.0	32.0
2	31.4323504260542	32.0	32.0	32.0	32.0	32.0
3	31.498520929683096	32.0	32.0	32.0	32.0	32.0
4	31.590398894009805	32.0	32.0	32.0	32.0	32.0
5	31.53643557623079	32.0	32.0	32.0	32.0	32.0
6	35.12090967480674	36.0	36.0	36.0	36.0	36.0
7	35.130360931471074	36.0	36.0	36.0	36.0	36.0
8	35.074163177188844	36.0	36.0	36.0	36.0	36.0
9	35.202844689368625	36.0	36.0	36.0	36.0	36.0
10	35.075906452726954	36.0	36.0	36.0	36.0	36.0
11	35.20647598127096	36.0	36.0	36.0	36.0	36.0
12	35.11413825196717	36.0	36.0	36.0	36.0	36.0
13	35.150016416376786	36.0	36.0	36.0	36.0	36.0
14	35.12387100825289	36.0	36.0	36.0	36.0	36.0
15	35.08210902283446	36.0	36.0	36.0	36.0	36.0
16	35.09162041079788	36.0	36.0	36.0	36.0	36.0
17	35.06937768362529	36.0	36.0	36.0	36.0	36.0
18	35.080747288371086	36.0	36.0	36.0	36.0	36.0
19	35.063288458355885	36.0	36.0	36.0	36.0	36.0
20	35.0591468486144	36.0	36.0	36.0	36.0	36.0
21	35.05246056743725	36.0	36.0	36.0	36.0	36.0
22	35.03442490265913	36.0	36.0	36.0	36.0	36.0
23	34.99728770591644	36.0	36.0	36.0	36.0	36.0
24	34.97146796465131	36.0	36.0	36.0	36.0	36.0
25	34.94484416681167	36.0	36.0	36.0	32.0	36.0
26	34.906354281907234	36.0	36.0	36.0	32.0	36.0
27	34.894216273658074	36.0	36.0	36.0	32.0	36.0
28	34.8764482729812	36.0	36.0	36.0	32.0	36.0
29	34.85928264956596	36.0	36.0	36.0	32.0	36.0
30	34.845780246176474	36.0	36.0	36.0	32.0	36.0
31	34.84802532538749	36.0	36.0	36.0	32.0	36.0
32	34.81408573020416	36.0	36.0	36.0	32.0	36.0
33	34.79431211124184	36.0	36.0	36.0	32.0	36.0
34	34.78564043292407	36.0	36.0	36.0	32.0	36.0
35	34.757275488087885	36.0	36.0	36.0	32.0	36.0
36	34.733176140539086	36.0	36.0	36.0	32.0	36.0
37	34.72605031552968	36.0	36.0	36.0	32.0	36.0
38	34.698732613141296	36.0	36.0	36.0	32.0	36.0
39	34.69662961407945	36.0	36.0	36.0	32.0	36.0
40	34.6905590135487	36.0	36.0	36.0	32.0	36.0
41	34.66107285944548	36.0	36.0	36.0	32.0	36.0
42	34.63521001521375	36.0	36.0	36.0	32.0	36.0
43	34.62768455652635	36.0	36.0	36.0	32.0	36.0
44	34.58908398746076	36.0	36.0	36.0	32.0	36.0
45	34.5648803413755	36.0	36.0	36.0	32.0	36.0
46	34.56381500632443	36.0	36.0	36.0	32.0	36.0
47	34.535305320662125	36.0	36.0	36.0	32.0	36.0
48	34.493577391908666	36.0	36.0	36.0	32.0	36.0
49	34.4854399775226	36.0	36.0	36.0	32.0	36.0
50	33.9585711313358	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	10.0
21	31.0
22	139.0
23	413.0
24	1095.0
25	2705.0
26	5735.0
27	10794.0
28	18015.0
29	28407.0
30	41683.0
31	59984.0
32	88541.0
33	145127.0
34	320675.0
35	1155864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.068934098439875	17.882715596017785	11.301128180892135	26.747222124650204
2	16.733080238024538	20.605981265838412	35.760142704032816	26.900795792104237
3	18.681221025027565	23.890968961392247	28.52983810200893	28.897971911571258
4	12.635242281372058	15.471259352284457	35.74621385468439	36.147284511659095
5	14.64107448835155	36.30506273879588	33.32925362650461	15.724609146347953
6	34.68096836024346	35.14860968764667	16.459878523939214	13.710543428170654
7	30.30592479858665	30.286501846510788	20.828588456912975	18.578984897989592
8	28.422149390625158	32.27794921406264	19.570875378680846	19.72902601663136
9	27.583025093908592	13.902622416416163	18.6127656087283	39.901586880946944
10	16.016849534993487	26.333358343696673	31.443082000467214	26.206710120842626
11	37.845894655285356	20.98720693308557	22.101658080662144	19.065240330966926
12	24.807247258305438	23.65842016452562	28.3270567964066	23.207275780762348
13	29.43187629342158	19.088068939204064	25.313733722643583	26.16632104473077
14	23.58328264743742	19.529262391171663	24.762015750143277	32.12543921124764
15	25.3130419466364	27.075314718173114	22.05988545253592	25.55175788265457
16	26.131412963137386	25.56053811659193	23.499205255794823	24.808843664475866
17	24.294055888051485	25.617795884571322	25.042610741365706	25.045537486011494
18	25.2721207351344	24.428579714679646	26.021633432150875	24.27766611803508
19	25.724276176138943	24.85082914675815	25.198047488826486	24.22684718827642
20	25.76381383562657	24.19449335655572	24.88403439510308	25.15765841271463
21	26.8557556562001	24.07891354981665	24.322259063729064	24.743071730254186
22	25.90972535960379	24.41080639264887	24.68054582191238	24.99892242583496
23	24.516488481131276	23.978127106923562	25.414254097841603	26.091130314103555
24	24.80251125333316	24.958426922645074	25.08438336949193	25.154678454529826
25	24.959650834042403	24.324121537594568	25.23061417470324	25.485613453659788
26	24.68192937392675	25.18761763517968	25.521745446650502	24.608707544243067
27	25.513869842876385	24.627013001663986	24.75994042212172	25.09917673333791
28	24.66548639037133	24.544638443269847	25.549363273398924	25.240511892959898
29	24.651225161915498	24.83263011641526	25.338158737051153	25.177985984618097
30	24.618073127109586	24.907395138730358	25.657759252371058	24.816772481788995
31	25.195759306648874	24.644945964311805	24.61690242925127	25.54239229978805
32	24.882544416010678	24.735515407714153	24.706567242490372	25.675372933784796
33	24.524789793217508	24.287883117525826	25.409518092869334	25.77780899638733
34	25.18868190595997	24.51702061652142	25.436444143610572	24.85785333390804
35	25.759397111888383	24.401600450399393	25.290692260250392	24.548310177461833
36	24.587901050488473	24.92325277335662	25.104444873700327	25.384401302454577
37	25.6419016177448	24.669903114109516	24.75871651072439	24.929478757421293
38	24.614933528307738	24.469447712642634	25.495936880228566	25.419681878821066
39	25.159361245963087	24.384412477297772	24.869932807264288	25.586293469474853
40	25.461348079869268	24.44433092222788	25.441765497512	24.652555500390854
41	24.388297065645816	24.76627283326442	25.86204602864698	24.98338407244278
42	25.587517380872182	24.908140128276557	25.260147688856183	24.244194801995082
43	24.669158124563317	24.00382924626747	25.62599076957952	25.70102185958969
44	24.397609434973322	24.54197776631913	25.282657015859233	25.777755782848317
45	24.391755945681748	24.840505720189377	25.46768049101197	25.300057843116907
46	24.313451492348683	24.355011310549752	25.606116157829394	25.725421039272167
47	24.422686008024606	24.277732250614616	25.983333510711894	25.316248230648885
48	25.024198856866754	24.98013804656291	25.18453124991685	24.81113184665348
49	24.15468962937302	24.93410833531554	25.423779321325167	25.487422713986273
50	23.993292965542636	25.26536261567958	25.351089627031627	25.390254791746152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	34.5
2	36.0
3	145.0
4	254.0
5	280.0
6	306.0
7	303.0
8	300.0
9	383.0
10	466.0
11	712.5
12	959.0
13	1628.0
14	2297.0
15	3010.5
16	3724.0
17	4320.5
18	4917.0
19	5361.5
20	5806.0
21	6936.0
22	8066.0
23	8825.0
24	9584.0
25	11423.5
26	13263.0
27	16161.5
28	19060.0
29	22116.0
30	25172.0
31	27344.0
32	29516.0
33	34238.5
34	38961.0
35	45119.0
36	51277.0
37	60463.5
38	69650.0
39	75361.0
40	81072.0
41	88847.5
42	96623.0
43	98598.0
44	100573.0
45	109824.0
46	119075.0
47	126447.5
48	133820.0
49	138155.0
50	142490.0
51	136821.5
52	131153.0
53	129309.0
54	127465.0
55	128989.5
56	130514.0
57	127888.0
58	125262.0
59	115110.5
60	104959.0
61	92581.5
62	80204.0
63	71318.5
64	62433.0
65	53522.0
66	44611.0
67	39737.0
68	34863.0
69	32350.0
70	29837.0
71	23307.5
72	16778.0
73	14969.0
74	13160.0
75	9709.0
76	6258.0
77	5688.5
78	5119.0
79	4455.0
80	3791.0
81	2936.0
82	2081.0
83	1869.0
84	1657.0
85	1360.5
86	1064.0
87	773.0
88	482.0
89	322.0
90	162.0
91	105.0
92	48.0
93	29.5
94	11.0
95	9.5
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014208014916819257
2	0.004629577894244477
3	6.917760071859563E-4
4	5.3213539014304335E-5
5	5.3213539014304335E-5
6	1.59640617042913E-4
7	5.3213539014304335E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0642707802860867E-4
47	5.3213539014304335E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1879221.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.10053879143643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.32028949737281	46.434323449755766
2	11.813361114316686	13.490985691305731
3	3.0865425179277017	5.287297223290458
4	1.2367274959812629	2.824712254348566
5	0.6253978506325425	1.7855277115062234
6	0.4086551004296435	1.4000655848640722
7	0.2732473517989782	1.0921799707738382
8	0.18306065470862568	0.8362289612300504
9	0.14223470663500756	0.7309510545330746
>10	0.7511098983154496	7.907567514500184
>50	0.07283227751726089	2.941089493325435
>100	0.07353791466921947	8.995898768223459
>500	0.009916429407552442	3.8048217737773595
>1k	0.003087190287256893	2.4683505485659123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2093	0.11137593715693896	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2063	0.10977953098650983	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2021	0.10754456234790906	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1924	0.10238284906352152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.3213539014304335E-5	0.0	5.3213539014304335E-5	0.0
6	0.0	5.3213539014304335E-5	0.0	5.3213539014304335E-5	5.3213539014304335E-5
7	0.0	5.3213539014304335E-5	0.0	5.3213539014304335E-5	5.3213539014304335E-5
8	0.0	5.3213539014304335E-5	0.0	5.3213539014304335E-5	5.3213539014304335E-5
9	0.0	5.3213539014304335E-5	0.0	5.3213539014304335E-5	5.3213539014304335E-5
10	0.0	5.3213539014304335E-5	0.0	1.0642707802860867E-4	5.3213539014304335E-5
11	0.0	5.3213539014304335E-5	0.0	1.0642707802860867E-4	5.3213539014304335E-5
12	0.0	5.3213539014304335E-5	0.0	1.0642707802860867E-4	2.1285415605721734E-4
13	0.0	5.3213539014304335E-5	0.0	1.0642707802860867E-4	2.6606769507152165E-4
14	0.0	5.3213539014304335E-5	0.0	1.0642707802860867E-4	2.6606769507152165E-4
15	0.0	5.3213539014304335E-5	0.0	1.59640617042913E-4	2.6606769507152165E-4
16	0.0	5.3213539014304335E-5	0.0	1.59640617042913E-4	2.6606769507152165E-4
17	0.0	5.3213539014304335E-5	0.0	1.59640617042913E-4	2.6606769507152165E-4
18	0.0	5.3213539014304335E-5	0.0	2.1285415605721734E-4	2.6606769507152165E-4
19	0.0	5.3213539014304335E-5	0.0	3.19281234085826E-4	2.6606769507152165E-4
20	0.0	5.3213539014304335E-5	0.0	4.78921851128739E-4	3.19281234085826E-4
21	0.0	5.3213539014304335E-5	0.0	6.917760071859563E-4	3.19281234085826E-4
22	0.0	5.3213539014304335E-5	0.0	0.0011706978583146952	4.257083121144347E-4
23	0.0	5.3213539014304335E-5	0.0	0.001756046787472043	4.257083121144347E-4
24	0.0	5.3213539014304335E-5	0.0	0.0026606769507152165	4.257083121144347E-4
25	0.0	5.3213539014304335E-5	0.0	0.0031395988018439553	4.257083121144347E-4
26	0.0	5.3213539014304335E-5	0.0	0.004310296660158651	4.78921851128739E-4
27	0.0	5.3213539014304335E-5	0.0	0.006066343447630694	4.78921851128739E-4
28	0.0	5.3213539014304335E-5	0.0	0.014048374299776343	4.78921851128739E-4
29	0.0	5.3213539014304335E-5	0.0	0.03176848279153969	4.78921851128739E-4
30	0.0	5.3213539014304335E-5	0.0	0.0663572831508375	4.78921851128739E-4
31	0.0	5.3213539014304335E-5	0.0	0.13239528506758919	4.78921851128739E-4
32	0.0	5.3213539014304335E-5	0.0	0.20955491663833045	4.78921851128739E-4
33	0.0	5.3213539014304335E-5	0.0	0.29017342824500153	4.78921851128739E-4
34	0.0	5.3213539014304335E-5	0.0	0.3831906944420055	5.321353901430433E-4
35	0.0	1.59640617042913E-4	0.0	0.49073525678991453	5.321353901430433E-4
36	0.0	1.59640617042913E-4	0.0	0.6510676498400135	5.321353901430433E-4
37	0.0	1.59640617042913E-4	0.0	0.8658374933017458	5.321353901430433E-4
38	0.0	1.59640617042913E-4	0.0	1.1555319996956186	5.853489291573476E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	345	0.0	19.76801	4
GTATCAA	3455	0.0	18.851456	1
TATCGAG	60	0.0074138255	18.333235	10
CGCAATA	585	0.0	17.675116	36
GTCGATA	115	4.275653E-6	17.217299	36
ATACGAA	610	0.0	16.950727	40
CGTCGTA	650	0.0	16.922983	10
AACGCAG	4105	0.0	15.863496	6
CGAATGC	640	0.0	15.812415	43
TAGCGGC	675	0.0	15.644359	30
CGGTTAA	85	0.0029808707	15.529328	44
CCGTCGT	710	0.0	15.492874	9
CTAGCGG	655	0.0	15.450298	29
TACGAAT	670	0.0	15.432753	41
CAATACG	675	0.0	15.318435	38
TCTAGCG	665	0.0	15.217961	28
ACCGTCG	725	0.0	15.172332	8
TCGATAT	145	2.2872246E-6	15.172331	37
ATACCGT	785	0.0	15.133677	6
GAGCGTT	175	6.415212E-8	15.085632	7
>>END_MODULE