FastQCFastQC Report
Thu 2 Feb 2017
SRR4062501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041964
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG65470.6283326487287468No Hit
TCCATGTACTCTGCGTTGATACCAC59750.5734363183372938No Hit
GTATCAACGCAGAGTACTTTTTTTT34390.3300497905877746No Hit
GAGTACATGGAAGCAGTGGTATCAA27210.26114145978171993No Hit
TATCAACGCAGAGTACTTTTTTTTT26840.25759047337527974No Hit
CATGTACTCTGCGTTGATACCACTG25530.24501806204437004No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25360.24338652774951916No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18810.18052447109497066No Hit
GCTTCCATGTACTCTGCGTTGATAC17720.17006345708680914No Hit
CCCATGTACTCTGCGTTGATACCAC16770.1609460595567601No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16170.15518770322199232No Hit
GTACATGGGAAGCAGTGGTATCAAC15960.15317227850482357No Hit
GCGTTGATACCACTGCTTCCATGTA15120.14511057963614865No Hit
CATGGAAGCAGTGGTATCAACGCAG14040.1347455382335666No Hit
GTACATGGGGTGGTATCAACGCAAA13540.12994690795459343No Hit
GTATCAACGCAGAGTACATGGAAGC12180.1168946335957864No Hit
GCGCAAGACGGACCAGAGCGAAAGC12060.11574296232883287No Hit
GAATAGGACCGCGGTTCTATTTTGT12050.1156469897232534No Hit
GGTATCAACGCAGAGTACTTTTTTT11930.11449531845629983No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11670.11200003071123378No Hit
GTCCTATTCCATTATTCCTAGCTGC11530.1106564142331213No Hit
GTATCTGATCGTCTTCGAACCTCCG11500.1103684964163829No Hit
ACTCTGCGTTGATACCACTGCTTCC11400.10940877036058827No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11340.10883293472711149No Hit
GAATAACGCCGCCGCATCGCCAGTC11170.10720140043226062No Hit
GATTAAGAGGGACGGCCGGGGGCAT10950.10509000310951241No Hit
GAACTACGACGGTATCTGATCGTCT10520.1009631810695955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC704.4794615E-714.91451
GAGCGTT602.562736E-514.2523077
TGTTTAA500.001497681713.30151410
AAGACGG2850.012.3323665
CGGTTCT2550.012.29551712
CGTCGTA1551.8189894E-1212.2594610
CGGACCA2650.012.1900669
CAAGACG2900.012.1185754
CGCAAGA2750.012.0835732
GTGTATA550.003089014212.0795131
GCAAGAC3350.011.905513
ACGGTAT2000.011.8763519
ATTTAGA802.8920038E-511.8638081
GCGCAAG2800.011.8638081
GTCCTAG1052.7497845E-711.7508191
AGCGAAA2850.011.66911617
CAGAGCG2850.011.66855514
GCGAAAG2900.011.46847518
GCGTTCC752.0703694E-411.4012989
CGGTCCA2200.011.2285510
Produced by FastQC (version 0.11.5)