##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062501_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041964
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03991212748233	32.0	32.0	32.0	32.0	32.0
2	30.834739971822444	32.0	32.0	32.0	32.0	32.0
3	30.878565862160304	32.0	32.0	32.0	32.0	32.0
4	30.77809502055733	32.0	32.0	32.0	32.0	32.0
5	30.810921490569733	32.0	32.0	32.0	32.0	32.0
6	34.279329228265084	36.0	36.0	36.0	32.0	36.0
7	34.15258396643262	36.0	36.0	36.0	32.0	36.0
8	34.16361409799187	36.0	36.0	36.0	32.0	36.0
9	34.208897812208484	36.0	36.0	36.0	32.0	36.0
10	34.04648913014269	36.0	36.0	36.0	32.0	36.0
11	34.290287380370145	36.0	36.0	36.0	32.0	36.0
12	34.11227835126741	36.0	36.0	36.0	32.0	36.0
13	34.209870014703	36.0	36.0	36.0	32.0	36.0
14	34.136642916645876	36.0	36.0	36.0	32.0	36.0
15	34.13718804104556	36.0	36.0	36.0	32.0	36.0
16	34.101021724359	36.0	36.0	36.0	32.0	36.0
17	34.0362392558668	36.0	36.0	36.0	32.0	36.0
18	34.0632517054332	36.0	36.0	36.0	32.0	36.0
19	34.012834416544145	36.0	36.0	36.0	32.0	36.0
20	33.99275022937453	36.0	36.0	36.0	32.0	36.0
21	33.912927893861976	36.0	36.0	36.0	32.0	36.0
22	33.876508209496684	36.0	36.0	36.0	32.0	36.0
23	33.85011286378416	36.0	36.0	36.0	32.0	36.0
24	33.83362477014561	36.0	36.0	36.0	32.0	36.0
25	33.22056808104695	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	36.0
5	101.0
6	171.0
7	48.0
8	163.0
9	176.0
10	116.0
11	35.0
12	79.0
13	57.0
14	267.0
15	504.0
16	817.0
17	1080.0
18	1622.0
19	2010.0
20	2589.0
21	3032.0
22	3861.0
23	4607.0
24	5770.0
25	7577.0
26	10091.0
27	13245.0
28	18358.0
29	25704.0
30	35053.0
31	50711.0
32	77604.0
33	117434.0
34	266147.0
35	392897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20000729445708	17.72447491906512	11.739372887846763	25.33614489863104
2	16.21152122295185	20.514080492313365	36.982622766421805	26.29177551831299
3	18.745879022002544	24.94267821580248	28.513088753989013	27.798354008205965
4	12.458198153231844	15.622660344397305	35.65078420456509	36.268357297805764
5	14.253058951724004	36.91776712124921	33.47071614752092	15.358457779505862
6	33.631245865215384	35.110310532526654	17.373056711241524	13.885386891016438
7	29.59201508935294	30.91300573149557	21.324659260283653	18.17031991886784
8	28.570605220538653	31.962687384370657	20.27410997827177	19.19259741681892
9	27.436829000253603	14.629235415722047	18.998658961136773	38.93527662288758
10	15.808822116400897	26.792652976513047	31.74985683373881	25.648668073347245
11	36.71202787970679	21.65539122004464	21.876384208801863	19.756196691446707
12	24.086707418679364	24.38874383410388	28.841450582018314	22.683098165198448
13	29.54422943324868	19.57171999027597	25.62627255527658	25.257778021198764
14	23.65645848327233	19.815105395305665	25.474079490651015	31.05435663077099
15	25.261939190714788	26.821900566393957	23.677669473815687	24.238490769075565
16	25.541021975234624	25.86154218645514	24.45484661916511	24.142589219145126
17	23.50711346764011	26.005138838976	26.262657321692473	24.225090371691415
18	24.806874953754733	24.46977697528557	27.46695084884692	23.25639722211278
19	24.71029039669963	26.366817187210206	25.854425909393612	23.06846650669655
20	24.95061130520676	23.94880871393376	26.756085186850694	24.344494794008785
21	26.132275274636573	24.91543730444877	24.59015920849317	24.362128212421492
22	24.792440788676945	25.075594035520066	25.3842176185524	24.747747557250587
23	23.305578300330986	24.831816801405434	25.833323722864844	26.02928117539874
24	23.493227958431753	26.41221139302058	25.523591152044684	24.570969496502986
25	24.45528399033666	24.885214205955215	25.402783303255518	25.256718500452607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	197.0
2	520.0
3	843.0
4	843.0
5	843.0
6	2274.5
7	3706.0
8	3706.0
9	3706.0
10	3763.0
11	3820.0
12	3820.0
13	3820.0
14	3699.5
15	3579.0
16	3579.0
17	3579.0
18	5805.0
19	8031.0
20	8031.0
21	8031.0
22	12862.5
23	17694.0
24	17694.0
25	17694.0
26	25836.5
27	33979.0
28	33979.0
29	33979.0
30	40698.0
31	47417.0
32	47417.0
33	47417.0
34	57324.5
35	67232.0
36	67232.0
37	67232.0
38	75225.0
39	83218.0
40	83218.0
41	83218.0
42	95921.0
43	108624.0
44	108624.0
45	108624.0
46	133768.5
47	158913.0
48	158913.0
49	158913.0
50	148473.0
51	138033.0
52	138033.0
53	138033.0
54	122023.5
55	106014.0
56	106014.0
57	106014.0
58	97366.0
59	88718.0
60	88718.0
61	88718.0
62	79014.0
63	69310.0
64	69310.0
65	69310.0
66	57914.5
67	46519.0
68	46519.0
69	46519.0
70	35731.0
71	24943.0
72	24943.0
73	24943.0
74	19743.0
75	14543.0
76	14543.0
77	14543.0
78	12103.0
79	9663.0
80	9663.0
81	9663.0
82	6787.5
83	3912.0
84	3912.0
85	3912.0
86	2906.5
87	1901.0
88	1901.0
89	1901.0
90	1256.5
91	612.0
92	612.0
93	612.0
94	350.0
95	88.0
96	88.0
97	88.0
98	271.5
99	455.0
100	455.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007389890629618682
2	0.0024952877450660485
3	0.0037429316175990727
4	0.01382005520344273
5	0.03349443934723272
6	0.047506439761834385
7	0.06718082390562437
8	0.08848674234426525
9	0.09405315346787413
10	0.11670268838462748
11	0.11583893493441232
12	0.13061871619364968
13	0.119293948735273
14	0.12121340084686226
15	0.1121919759223927
16	0.11094433204985969
17	0.12111742824128281
18	0.1263959215481533
19	0.12985093534901399
20	0.11900603091853461
21	0.12629994894257382
22	0.1476058673812147
23	0.13743277118979158
24	0.13071468879922915
25	0.1276435654206863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1041964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.223277794004176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64263541633564	39.9991420276122
2	12.397006267932852	12.45236579216805
3	3.518596230981102	5.301463078604998
4	1.4029480102515672	2.818425905976395
5	0.7291092025583714	1.8309127011126973
6	0.4370426417949453	1.3169828404015798
7	0.2929231060528116	1.029809096930103
8	0.21880661915109037	0.8791348493433668
9	0.16034075552042076	0.7247554475582338
>10	0.9608838369332247	9.12715486458222
>50	0.11003774158221333	3.850834582913648
>100	0.11365209109309385	11.862064615321225
>500	0.010488028448781122	3.525827212206176
>1k	0.005148668511219823	4.0818764528436615
>5k	3.8138285268294984E-4	1.1992505324255256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6547	0.6283326487287468	No Hit
TCCATGTACTCTGCGTTGATACCAC	5975	0.5734363183372938	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3439	0.3300497905877746	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2721	0.26114145978171993	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2684	0.25759047337527974	No Hit
CATGTACTCTGCGTTGATACCACTG	2553	0.24501806204437004	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2536	0.24338652774951916	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1881	0.18052447109497066	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1772	0.17006345708680914	No Hit
CCCATGTACTCTGCGTTGATACCAC	1677	0.1609460595567601	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1617	0.15518770322199232	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1596	0.15317227850482357	No Hit
GCGTTGATACCACTGCTTCCATGTA	1512	0.14511057963614865	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1404	0.1347455382335666	No Hit
GTACATGGGGTGGTATCAACGCAAA	1354	0.12994690795459343	No Hit
GTATCAACGCAGAGTACATGGAAGC	1218	0.1168946335957864	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1206	0.11574296232883287	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1205	0.1156469897232534	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1193	0.11449531845629983	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1167	0.11200003071123378	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1153	0.1106564142331213	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1150	0.1103684964163829	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1140	0.10940877036058827	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1134	0.10883293472711149	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1117	0.10720140043226062	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1095	0.10509000310951241	No Hit
GAACTACGACGGTATCTGATCGTCT	1052	0.1009631810695955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.59726055794634E-5	0.0
8	0.0	0.0	0.0	9.59726055794634E-5	0.0
9	0.0	0.0	0.0	9.59726055794634E-5	0.0
10	0.0	0.0	0.0	9.59726055794634E-5	0.0
11	0.0	0.0	0.0	9.59726055794634E-5	0.0
12	0.0	0.0	0.0	9.59726055794634E-5	1.919452111589268E-4
13	0.0	0.0	0.0	9.59726055794634E-5	1.919452111589268E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAC	70	4.4794615E-7	14.9145	1
GAGCGTT	60	2.562736E-5	14.252307	7
TGTTTAA	50	0.0014976817	13.301514	10
AAGACGG	285	0.0	12.332366	5
CGGTTCT	255	0.0	12.295517	12
CGTCGTA	155	1.8189894E-12	12.25946	10
CGGACCA	265	0.0	12.190066	9
CAAGACG	290	0.0	12.118575	4
CGCAAGA	275	0.0	12.083573	2
GTGTATA	55	0.0030890142	12.079513	1
GCAAGAC	335	0.0	11.90551	3
ACGGTAT	200	0.0	11.876351	9
ATTTAGA	80	2.8920038E-5	11.863808	1
GCGCAAG	280	0.0	11.863808	1
GTCCTAG	105	2.7497845E-7	11.750819	1
AGCGAAA	285	0.0	11.669116	17
CAGAGCG	285	0.0	11.668555	14
GCGAAAG	290	0.0	11.468475	18
GCGTTCC	75	2.0703694E-4	11.401298	9
CGGTCCA	220	0.0	11.22855	10
>>END_MODULE