FastQCFastQC Report
Thu 2 Feb 2017
SRR4062501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041964
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA34650.3325450783328407No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC29010.27841652878602335No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12650.12140534605802121No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12270.1177583870460016No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC12000.11516712669535607No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11620.11152016768333646No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11540.11075238683870077No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10990.10547389353183027No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10750.10317055099792316No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10490.10067526325285711No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG10480.10057929064727764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG300.005744898729.33307837
CGTAATG407.031834E-427.4997642
TAACGCT450.001398155624.44423143
ACCGTAT551.5935067E-423.9997928
ATTCGTT550.0044834319.99982640
CGCAATA3700.019.6214536
ATACGAA3850.018.8569840
CTTGCGT708.122774E-418.85697710
CGAATGC4000.018.69983743
TAGCGGC3800.018.52615430
CGATTAA600.00741180518.33317441
CTAGCGG3850.018.28555529
AATACGA4000.018.14984339
TCTAGCG3900.018.05112628
GAGCGTT1355.523907E-817.925777
CAATACG4050.017.9257738
CGGTCCA4200.017.80936810
TACGAAT4150.017.49382441
CGAGCCG3900.017.48702815
CCCCTAG902.2102821E-417.1158891
Produced by FastQC (version 0.11.5)