##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041964
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.123007128845142	32.0	32.0	32.0	32.0	32.0
2	31.21660057353229	32.0	32.0	32.0	32.0	32.0
3	31.365146972448184	32.0	32.0	32.0	32.0	32.0
4	31.5096807567248	32.0	32.0	32.0	32.0	32.0
5	31.39040216360642	32.0	32.0	32.0	32.0	32.0
6	34.96150730735419	36.0	36.0	36.0	36.0	36.0
7	34.975593206675086	36.0	36.0	36.0	36.0	36.0
8	34.9066052186064	36.0	36.0	36.0	32.0	36.0
9	35.05327247390505	36.0	36.0	36.0	36.0	36.0
10	34.90476254457928	36.0	36.0	36.0	32.0	36.0
11	35.06822308640222	36.0	36.0	36.0	36.0	36.0
12	34.95287553120837	36.0	36.0	36.0	32.0	36.0
13	35.009730662479704	36.0	36.0	36.0	36.0	36.0
14	34.9662617902346	36.0	36.0	36.0	32.0	36.0
15	34.934160873120376	36.0	36.0	36.0	32.0	36.0
16	34.93833184255886	36.0	36.0	36.0	32.0	36.0
17	34.90609272489261	36.0	36.0	36.0	32.0	36.0
18	34.92101550533416	36.0	36.0	36.0	32.0	36.0
19	34.88815064627953	36.0	36.0	36.0	32.0	36.0
20	34.87350330721599	36.0	36.0	36.0	32.0	36.0
21	34.84815022400006	36.0	36.0	36.0	32.0	36.0
22	34.83290113669954	36.0	36.0	36.0	32.0	36.0
23	34.78469601636909	36.0	36.0	36.0	32.0	36.0
24	34.76209734693329	36.0	36.0	36.0	32.0	36.0
25	34.73539872778714	36.0	36.0	36.0	32.0	36.0
26	34.68792779788937	36.0	36.0	36.0	32.0	36.0
27	34.665523952842904	36.0	36.0	36.0	32.0	36.0
28	34.641953080912586	36.0	36.0	36.0	32.0	36.0
29	34.59051464350016	36.0	36.0	36.0	32.0	36.0
30	34.589050101539016	36.0	36.0	36.0	32.0	36.0
31	34.57316375613745	36.0	36.0	36.0	32.0	36.0
32	34.522379851895074	36.0	36.0	36.0	32.0	36.0
33	34.47380139812892	36.0	36.0	36.0	32.0	36.0
34	34.43840958036938	36.0	36.0	36.0	32.0	36.0
35	34.38627342211439	36.0	36.0	36.0	32.0	36.0
36	34.35060136434656	36.0	36.0	36.0	32.0	36.0
37	34.31107312728655	36.0	36.0	36.0	32.0	36.0
38	34.24986275917402	36.0	36.0	36.0	32.0	36.0
39	34.184250127643566	36.0	36.0	36.0	32.0	36.0
40	34.14788322821134	36.0	36.0	36.0	32.0	36.0
41	34.10183173315009	36.0	36.0	36.0	32.0	36.0
42	34.01001282194011	36.0	36.0	36.0	32.0	36.0
43	34.010649120315094	36.0	36.0	36.0	32.0	36.0
44	33.979211373905436	36.0	36.0	36.0	32.0	36.0
45	33.83228787174989	36.0	36.0	36.0	32.0	36.0
46	33.89595705801736	36.0	36.0	36.0	32.0	36.0
47	33.80714400881413	36.0	36.0	36.0	32.0	36.0
48	33.77218598723181	36.0	36.0	36.0	32.0	36.0
49	33.769655189622675	36.0	36.0	36.0	32.0	36.0
50	33.15299185000634	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	1.0
20	8.0
21	27.0
22	118.0
23	317.0
24	926.0
25	2095.0
26	4629.0
27	8516.0
28	14923.0
29	22441.0
30	31701.0
31	44995.0
32	65905.0
33	101374.0
34	204758.0
35	539228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.638602718504	18.372890596579268	12.34585913346125	25.64264755145548
2	15.673872879525877	21.17071766201982	36.545816637473905	26.6095928209804
3	18.365885797809703	25.05962326732626	29.0065156114588	27.567975323405243
4	12.34794325710222	16.09394959321972	35.96787985753813	35.59022729213993
5	14.16488477528974	37.000414601656104	33.295296190655336	15.539404432398815
6	33.742113435998625	35.583543353788336	16.946203412408515	13.728139797804525
7	29.434346517102817	31.061179715594506	21.256129056406035	18.248344710896642
8	28.549642789962032	32.141801444195764	19.996468208114678	19.312087557727523
9	27.4684154155038	14.327750286958091	18.90804288823798	39.29579140930013
10	15.813502193933763	26.910526659270378	31.605218606400985	25.67075254039487
11	37.07805643956989	21.45669140200621	21.82944900207685	19.635803156347052
12	24.07357643834144	24.430498558491465	28.898119320821063	22.59780568234603
13	29.729913893378274	19.529465509365007	25.553569988982343	25.187050608274376
14	23.75158834662234	19.639258170147915	25.429477409968097	31.179676073261646
15	25.31642168059549	26.830773424033843	23.601967054523957	24.25083784084671
16	25.83544153156923	25.700983911152402	24.389134365486715	24.074440191791656
17	23.651872809425278	26.05157183933418	26.163763815256573	24.13279153598397
18	25.12908315450438	24.242392251555717	27.447781305304215	23.180743288635693
19	24.979653807617154	26.22086751557635	25.912123643427222	22.887355033379272
20	25.112383921133553	23.744966236837357	26.920507810250644	24.22214203177845
21	26.293902668422326	24.771873116537616	24.65152346914097	24.282700745899092
22	24.83003251551877	24.957580108333875	25.61556829218668	24.596819083960675
23	23.457528283126862	24.375794173311167	26.08928907332691	26.077388470235057
24	23.64669028872399	26.004353317389082	25.6790061844747	24.669950209412224
25	24.774176459071523	24.464856751288913	25.528905029348426	25.232061760291142
26	23.707632893266947	25.186954635668794	26.38833971231252	24.717072758751744
27	25.291468803144827	25.277936665758126	25.526505714208938	23.90408881688811
28	24.065226821656026	24.24719088183469	26.590170101846127	25.097412194663153
29	23.568184697359985	25.561055852217546	26.900641480895693	23.97011796952678
30	24.655938208997625	24.889055667950142	26.704473475091273	23.750532647960966
31	24.077799232986933	25.254999213024636	25.463355739737647	25.20384581425078
32	23.980770928746097	25.312294858555575	25.687547746371276	25.019386466327052
33	23.796887416455846	24.71217815586719	26.06529592193204	25.425638505744917
34	24.023862628651276	25.462396013681854	26.62059341781482	23.89314793985205
35	25.547619687436416	24.81055007658614	25.92018534229589	23.72164489368155
36	24.174539619411036	25.70443892495326	25.68677996552664	24.434241490109063
37	25.463835602765545	25.36143283261226	25.571037003197805	23.603694561424387
38	24.2522774299304	25.231485924657665	25.598005305365636	24.918231340046297
39	24.423492558284163	25.151060881182076	25.707414075726227	24.718032484807537
40	25.057103700319782	25.299914392435824	25.533799632232974	24.10918227501142
41	23.305123785466677	25.86240983373706	26.328932669458833	24.503533711337436
42	25.673631718562252	25.675743115885002	25.526313768997777	23.124311396554965
43	24.543074424836174	24.271567923651872	25.647335224633483	25.53802242687847
44	24.167533619203734	25.061326494965275	26.052435592784395	24.718704293046592
45	24.609967330925063	25.364312010779642	25.5369667282171	24.4887539300782
46	23.847103975860946	25.106649660304637	26.219165171891902	24.827081191942515
47	24.45585879728935	24.59981784381979	26.53731466472418	24.407008694166684
48	24.793850843215314	26.685470899186537	24.659393222798485	23.861285034799668
49	23.60657373959177	25.50433604232008	26.45072190593917	24.43836831214898
50	23.546974751526925	26.66886763842129	24.72388681374788	25.0602707963039
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	19.0
2	23.0
3	73.5
4	124.0
5	145.5
6	167.0
7	177.0
8	187.0
9	298.0
10	409.0
11	570.0
12	731.0
13	1397.0
14	2063.0
15	2910.5
16	3758.0
17	4522.5
18	5287.0
19	5816.0
20	6345.0
21	6990.0
22	7635.0
23	7964.0
24	8293.0
25	8515.0
26	8737.0
27	10073.5
28	11410.0
29	13210.5
30	15011.0
31	16571.5
32	18132.0
33	20755.5
34	23379.0
35	26327.0
36	29275.0
37	34325.5
38	39376.0
39	42711.5
40	46047.0
41	50890.0
42	55733.0
43	56864.5
44	57996.0
45	66033.0
46	74070.0
47	80494.0
48	86918.0
49	85377.5
50	83837.0
51	76055.5
52	68274.0
53	65321.0
54	62368.0
55	63310.0
56	64252.0
57	62827.5
58	61403.0
59	56308.5
60	51214.0
61	45087.0
62	38960.0
63	34403.0
64	29846.0
65	25676.0
66	21506.0
67	19424.0
68	17342.0
69	16229.0
70	15116.0
71	11986.0
72	8856.0
73	7990.5
74	7125.0
75	5227.5
76	3330.0
77	3039.0
78	2748.0
79	2323.5
80	1899.0
81	1525.5
82	1152.0
83	962.0
84	772.0
85	624.0
86	476.0
87	358.0
88	240.0
89	163.5
90	87.0
91	54.5
92	22.0
93	15.0
94	8.0
95	5.5
96	3.0
97	4.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01353213738670434
2	0.0037429316175990727
3	4.7986302789731697E-4
4	9.59726055794634E-5
5	0.0
6	1.919452111589268E-4
7	9.59726055794634E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.59726055794634E-5
47	9.59726055794634E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1041964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.44093277766412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93762369066614	44.87257354218096
2	11.85502764907394	13.145075819393155
3	3.1731627808545944	5.277693132778358
4	1.307502582769037	2.8995665119168157
5	0.6778902769364531	1.8791434637133004
6	0.40613698602010956	1.3509968004278405
7	0.27090407514964504	1.0513422233696716
8	0.20335354483385465	0.9019288167386754
9	0.13609527823072318	0.6790724254572318
>10	0.8337041191998046	8.794597285167061
>50	0.09271044268890062	3.611233003151519
>100	0.09547330439623078	10.724649177397923
>500	0.00833221534454407	3.110242514013486
>1k	0.0020830538361360177	1.7018852842940306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3465	0.3325450783328407	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2901	0.27841652878602335	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1265	0.12140534605802121	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1227	0.1177583870460016	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1200	0.11516712669535607	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1162	0.11152016768333646	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1154	0.11075238683870077	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1099	0.10547389353183027	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1075	0.10317055099792316	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1049	0.10067526325285711	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1048	0.10057929064727764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.59726055794634E-5	0.0
8	0.0	0.0	0.0	9.59726055794634E-5	0.0
9	0.0	0.0	0.0	9.59726055794634E-5	0.0
10	0.0	0.0	0.0	9.59726055794634E-5	0.0
11	0.0	0.0	0.0	9.59726055794634E-5	0.0
12	0.0	0.0	0.0	9.59726055794634E-5	0.0
13	0.0	0.0	0.0	9.59726055794634E-5	0.0
14	0.0	0.0	0.0	9.59726055794634E-5	0.0
15	0.0	0.0	0.0	9.59726055794634E-5	9.59726055794634E-5
16	0.0	0.0	0.0	1.919452111589268E-4	9.59726055794634E-5
17	0.0	0.0	0.0	2.879178167383902E-4	9.59726055794634E-5
18	0.0	0.0	0.0	2.879178167383902E-4	1.919452111589268E-4
19	0.0	0.0	0.0	2.879178167383902E-4	1.919452111589268E-4
20	0.0	0.0	0.0	8.637534502151706E-4	2.879178167383902E-4
21	0.0	0.0	0.0	0.0013436164781124875	2.879178167383902E-4
22	0.0	0.0	0.0	0.0019194521115892679	4.7986302789731697E-4
23	0.0	0.0	0.0	0.0027832055618044384	4.7986302789731697E-4
24	0.0	0.0	0.0	0.005278493306870487	4.7986302789731697E-4
25	0.0	0.0	0.0	0.006910027601721365	4.7986302789731697E-4
26	0.0	0.0	0.0	0.009213370135628486	6.718082390562437E-4
27	0.0	0.0	0.0	0.011516712669535608	6.718082390562437E-4
28	0.0	0.0	0.0	0.01775493203220073	6.718082390562437E-4
29	0.0	0.0	0.0	0.02965553512405419	6.718082390562437E-4
30	0.0	0.0	0.0	0.05364868651892004	6.718082390562437E-4
31	0.0	0.0	0.0	0.09971553719706247	7.677808446357072E-4
32	0.0	0.0	0.0	0.15662729230568426	8.637534502151706E-4
33	0.0	0.0	0.0	0.2131551569919882	8.637534502151706E-4
34	0.0	0.0	0.0	0.28513461117658573	0.0010556986613740973
35	0.0	0.0	0.0	0.3621046408513154	0.0010556986613740973
36	0.0	0.0	0.0	0.4743925893792876	0.0010556986613740973
37	0.0	0.0	0.0	0.628140703517588	0.0010556986613740973
38	9.59726055794634E-5	0.0	0.0	0.8292033122065637	0.0010556986613740973
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	30	0.0057448987	29.333078	37
CGTAATG	40	7.031834E-4	27.49976	42
TAACGCT	45	0.0013981556	24.444231	43
ACCGTAT	55	1.5935067E-4	23.999792	8
ATTCGTT	55	0.00448343	19.999826	40
CGCAATA	370	0.0	19.62145	36
ATACGAA	385	0.0	18.85698	40
CTTGCGT	70	8.122774E-4	18.856977	10
CGAATGC	400	0.0	18.699837	43
TAGCGGC	380	0.0	18.526154	30
CGATTAA	60	0.007411805	18.333174	41
CTAGCGG	385	0.0	18.285555	29
AATACGA	400	0.0	18.149843	39
TCTAGCG	390	0.0	18.051126	28
GAGCGTT	135	5.523907E-8	17.92577	7
CAATACG	405	0.0	17.92577	38
CGGTCCA	420	0.0	17.809368	10
TACGAAT	415	0.0	17.493824	41
CGAGCCG	390	0.0	17.487028	15
CCCCTAG	90	2.2102821E-4	17.115889	1
>>END_MODULE