FastQCFastQC Report
Thu 2 Feb 2017
SRR4062500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730711
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG40560.5550758097250487No Hit
TCCATGTACTCTGCGTTGATACCAC31500.4310869824048084No Hit
GAGTACATGGAAGCAGTGGTATCAA18460.2526306569902465No Hit
CATGTACTCTGCGTTGATACCACTG15000.2052795154308612No Hit
GTATCAACGCAGAGTACTTTTTTTT13910.19036253730955194No Hit
GCTTCCATGTACTCTGCGTTGATAC11970.16381305331382723No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10930.14958034024395417No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10620.1453378969250497No Hit
GCGCAAGACGGACCAGAGCGAAAGC10010.13698986329752802No Hit
GAATAGGACCGCGGTTCTATTTTGT9920.13575818620494287No Hit
CCCATGTACTCTGCGTTGATACCAC9820.13438965610207046No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9760.13356853804034702No Hit
GGGTAGGCACACGCTGAGCCAGTCA9740.13329483201977252No Hit
TATCAACGCAGAGTACTTTTTTTTT9640.13192630191690013No Hit
CATGGAAGCAGTGGTATCAACGCAG9350.12795756461857014No Hit
GCGTTGATACCACTGCTTCCATGTA9060.12398882732024014No Hit
GAATAACGCCGCCGCATCGCCAGTC8790.12029379604248465No Hit
GTACATGGGAAGCAGTGGTATCAAC8480.11605135272358018No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC8310.11372485154869708No Hit
GTATCTGATCGTCTTCGAACCTCCG8180.11194576241496297No Hit
GAACTACGACGGTATCTGATCGTCT8130.11126149736352675No Hit
GTCCTATTCCATTATTCCTAGCTGC7980.10920870220921815No Hit
GTATCAACGCAGAGTACATGGAAGC7460.10209234567428163No Hit
GTACATGGGGAATAATTGCAATCCC7440.10181863965370713No Hit
GATTAAGAGGGACGGCCGGGGGCAT7410.10140808062284541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCA1200.015.83904716
CAAGACG2250.015.1940264
AAGACGG2300.014.8657575
AATCGCT456.7447935E-414.78108415
CGCCAGT1400.014.25612118
TCGCCAG1350.014.08011817
CGCAAGA2250.013.9230862
GTAAACG707.2178227E-613.57725717
GCATCGC1400.013.57446514
GTATTAG1205.2750693E-1113.4493561
ATCGCTC500.001494147913.304816
AAATCGC500.001495693113.30297714
TCGCGTA500.001497239613.3011529
GCAAGAC2450.013.174883
CATCGCC1450.013.106379515
CGCATCG1450.013.10548213
CCGTCAA801.985658E-613.0681118
AAACGCT801.985658E-613.0681119
GCGCAAG2400.013.0537871
CGACCCG957.390372E-812.9966715
Produced by FastQC (version 0.11.5)