##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062500_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730711
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084476626190106	32.0	32.0	32.0	32.0	32.0
2	30.67505894943418	32.0	32.0	32.0	32.0	32.0
3	30.672434108696873	32.0	32.0	32.0	32.0	32.0
4	30.741525719470488	32.0	32.0	32.0	32.0	32.0
5	30.496591675778795	32.0	32.0	32.0	32.0	32.0
6	34.14587709778558	36.0	36.0	36.0	32.0	36.0
7	33.9905653534708	36.0	36.0	36.0	32.0	36.0
8	33.9800837814129	36.0	36.0	36.0	32.0	36.0
9	34.18890368421989	36.0	36.0	36.0	32.0	36.0
10	33.787296208761056	36.0	36.0	36.0	32.0	36.0
11	34.18572048320061	36.0	36.0	36.0	32.0	36.0
12	33.91738046915949	36.0	36.0	36.0	32.0	36.0
13	34.07038487172083	36.0	36.0	36.0	32.0	36.0
14	33.95193995984733	36.0	36.0	36.0	32.0	36.0
15	33.87571009605713	36.0	36.0	36.0	32.0	36.0
16	33.8796008271396	36.0	36.0	36.0	32.0	36.0
17	33.76769612062772	36.0	36.0	36.0	32.0	36.0
18	33.80395669423343	36.0	36.0	36.0	32.0	36.0
19	33.80251973762541	36.0	36.0	36.0	32.0	36.0
20	33.786189067907834	36.0	36.0	36.0	32.0	36.0
21	33.733615615475884	36.0	36.0	36.0	32.0	36.0
22	33.69339725281267	36.0	36.0	36.0	27.0	36.0
23	33.63571918309701	36.0	36.0	36.0	27.0	36.0
24	33.603493036234575	36.0	36.0	36.0	27.0	36.0
25	33.07793642082848	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	36.0
5	69.0
6	108.0
7	38.0
8	105.0
9	122.0
10	86.0
11	30.0
12	45.0
13	47.0
14	146.0
15	179.0
16	276.0
17	394.0
18	553.0
19	829.0
20	1194.0
21	1789.0
22	2690.0
23	3814.0
24	5418.0
25	7365.0
26	10315.0
27	13003.0
28	17637.0
29	22976.0
30	29423.0
31	39661.0
32	55789.0
33	77824.0
34	163317.0
35	275429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.110325798345556	16.642623686760576	10.658659031309709	25.588391483584168
2	16.77476128629632	19.68161469959093	36.48949550217809	27.05412851193466
3	19.74229132166368	23.68757441799992	27.730986628710635	28.839147631625767
4	12.426413725610965	14.73600646039926	35.333730264643684	37.50384954934609
5	14.46606832879743	36.70222377982334	33.38955299889933	15.44215489247989
6	35.40780170973872	34.389871906506535	16.521404592526874	13.680921791227869
7	30.691027407202505	30.097665718127864	20.4799478531892	18.731359021480433
8	29.09670411851641	32.075776647632566	19.447933345477907	19.379585888373125
9	27.20214351466269	14.150648752904047	18.119328891421556	40.527878841011706
10	15.874479521330892	26.406131612082916	31.493018495403895	26.226370371182302
11	38.461190661809134	20.829114505050946	21.146297318811886	19.56339751432803
12	24.27612440342182	23.565095020875955	28.616431917806157	23.54234865789607
13	29.862562118332768	18.84697961381476	25.281253725044767	26.0092045428077
14	23.822416358609125	19.37638557386393	24.44282461135424	32.35837345617271
15	25.645841896150156	26.725167831209756	22.597890121934512	25.031100150705576
16	26.450722826861323	25.487456022095817	23.679155843444143	24.382665307598725
17	24.256823413350872	25.64411377836238	25.37912419160364	24.71993861668311
18	25.726445834806366	23.985113444215152	26.48488735053838	23.8035533704401
19	25.517185622260257	25.77151337344659	24.590451472867368	24.120849531425783
20	25.19998026916152	23.916379610370747	25.728465297017628	25.1551748234501
21	26.59805369734473	24.534868743782493	23.67558516512879	25.191492393743985
22	25.332803387995366	24.668909797364435	24.741000637304957	25.257286177335242
23	23.998514490568933	24.16556578175619	25.113605841176568	26.722313886498313
24	24.297551691014554	25.726085925820364	24.629985652994304	25.34637673017078
25	25.217668934936377	24.474977321870487	24.470592426737994	25.836761316455142
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	104.0
2	250.5
3	397.0
4	397.0
5	397.0
6	1026.5
7	1656.0
8	1656.0
9	1656.0
10	1685.0
11	1714.0
12	1714.0
13	1714.0
14	1815.5
15	1917.0
16	1917.0
17	1917.0
18	3017.0
19	4117.0
20	4117.0
21	4117.0
22	6739.5
23	9362.0
24	9362.0
25	9362.0
26	14205.0
27	19048.0
28	19048.0
29	19048.0
30	24072.5
31	29097.0
32	29097.0
33	29097.0
34	35679.5
35	42262.0
36	42262.0
37	42262.0
38	48354.5
39	54447.0
40	54447.0
41	54447.0
42	63901.0
43	73355.0
44	73355.0
45	73355.0
46	89557.5
47	105760.0
48	105760.0
49	105760.0
50	102202.0
51	98644.0
52	98644.0
53	98644.0
54	90002.5
55	81361.0
56	81361.0
57	81361.0
58	75998.0
59	70635.0
60	70635.0
61	70635.0
62	63640.0
63	56645.0
64	56645.0
65	56645.0
66	47418.5
67	38192.0
68	38192.0
69	38192.0
70	28698.5
71	19205.0
72	19205.0
73	19205.0
74	14982.5
75	10760.0
76	10760.0
77	10760.0
78	8878.5
79	6997.0
80	6997.0
81	6997.0
82	4877.0
83	2757.0
84	2757.0
85	2757.0
86	2044.5
87	1332.0
88	1332.0
89	1332.0
90	906.0
91	480.0
92	480.0
93	480.0
94	279.5
95	79.0
96	79.0
97	79.0
98	233.5
99	388.0
100	388.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010263975771543058
2	0.003284472246893779
3	0.005610973421776872
4	0.014506419090447522
5	0.03325528149979951
6	0.042013874158182925
7	0.06363664978356696
8	0.0845751603575148
9	0.09511284214963234
10	0.11687247078530363
11	0.11605135272358018
12	0.1264521815054105
13	0.11687247078530363
14	0.11892526593961224
15	0.11098779134295228
16	0.1093455552195054
17	0.11919897196018674
18	0.12658903451569772
19	0.1291892417111553
20	0.12166232614535706
21	0.1254942104333998
22	0.14725383906907108
23	0.13616874523580458
24	0.1289155356905808
25	0.12768385859799566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	730711.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.36223762169897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58424143130229	40.87624719337208
2	12.256950702982977	12.590888290481239
3	3.5693475905470966	5.499890373003559
4	1.4550370586568677	2.9893583662044794
5	0.7442732496160673	1.911376975112726
6	0.4468049215128799	1.3769340329573458
7	0.33309679998021446	1.1976017894127917
8	0.2109942621385271	0.8669709943019256
9	0.1639657356489642	0.7579482368596899
>10	0.9892734398551885	9.589089818828093
>50	0.11740408836191496	4.201222754085254
>100	0.11473570402646903	11.805214719570658
>500	0.011473570402621107	4.103915239057011
>1k	0.0024014449679904642	2.2333412167531494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4056	0.5550758097250487	No Hit
TCCATGTACTCTGCGTTGATACCAC	3150	0.4310869824048084	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1846	0.2526306569902465	No Hit
CATGTACTCTGCGTTGATACCACTG	1500	0.2052795154308612	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1391	0.19036253730955194	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1197	0.16381305331382723	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1093	0.14958034024395417	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1062	0.1453378969250497	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1001	0.13698986329752802	No Hit
GAATAGGACCGCGGTTCTATTTTGT	992	0.13575818620494287	No Hit
CCCATGTACTCTGCGTTGATACCAC	982	0.13438965610207046	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	976	0.13356853804034702	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	974	0.13329483201977252	No Hit
TATCAACGCAGAGTACTTTTTTTTT	964	0.13192630191690013	No Hit
CATGGAAGCAGTGGTATCAACGCAG	935	0.12795756461857014	No Hit
GCGTTGATACCACTGCTTCCATGTA	906	0.12398882732024014	No Hit
GAATAACGCCGCCGCATCGCCAGTC	879	0.12029379604248465	No Hit
GTACATGGGAAGCAGTGGTATCAAC	848	0.11605135272358018	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	831	0.11372485154869708	No Hit
GTATCTGATCGTCTTCGAACCTCCG	818	0.11194576241496297	No Hit
GAACTACGACGGTATCTGATCGTCT	813	0.11126149736352675	No Hit
GTCCTATTCCATTATTCCTAGCTGC	798	0.10920870220921815	No Hit
GTATCAACGCAGAGTACATGGAAGC	746	0.10209234567428163	No Hit
GTACATGGGGAATAATTGCAATCCC	744	0.10181863965370713	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	741	0.10140808062284541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.3685301028724077E-4	0.0
8	0.0	0.0	0.0	1.3685301028724077E-4	0.0
9	0.0	0.0	0.0	2.7370602057448154E-4	0.0
10	0.0	0.0	0.0	4.1055903086172237E-4	0.0
11	0.0	0.0	0.0	4.1055903086172237E-4	0.0
12	0.0	0.0	0.0	4.1055903086172237E-4	4.1055903086172237E-4
13	0.0	0.0	0.0	4.1055903086172237E-4	4.1055903086172237E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCCA	120	0.0	15.839047	16
CAAGACG	225	0.0	15.194026	4
AAGACGG	230	0.0	14.865757	5
AATCGCT	45	6.7447935E-4	14.781084	15
CGCCAGT	140	0.0	14.256121	18
TCGCCAG	135	0.0	14.080118	17
CGCAAGA	225	0.0	13.923086	2
GTAAACG	70	7.2178227E-6	13.577257	17
GCATCGC	140	0.0	13.574465	14
GTATTAG	120	5.2750693E-11	13.449356	1
ATCGCTC	50	0.0014941479	13.3048	16
AAATCGC	50	0.0014956931	13.302977	14
TCGCGTA	50	0.0014972396	13.301152	9
GCAAGAC	245	0.0	13.17488	3
CATCGCC	145	0.0	13.1063795	15
CGCATCG	145	0.0	13.105482	13
CCGTCAA	80	1.985658E-6	13.06811	18
AAACGCT	80	1.985658E-6	13.06811	19
GCGCAAG	240	0.0	13.053787	1
CGACCCG	95	7.390372E-8	12.996671	5
>>END_MODULE