FastQCFastQC Report
Thu 2 Feb 2017
SRR4062500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730711
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17030.23306067651917106No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14590.1996685420090843No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11110.1520436944291245No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10810.14793810412050729No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10760.14725383906907108No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10080.1379478343695387No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9910.1356213331946556No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG8500.11632505874415466No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8030.10989296726065434No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8010.10961926124007987No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7360.10072381557140922No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT7320.10017640353026025No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7320.10017640353026025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTC353.216861E-431.4281831
CTACGCT609.747031E-625.66634633
ATTTCGT551.5929117E-423.999742
TACGCTC651.8122926E-523.69201334
CGAGCCG3550.022.3095815
CCTAAGC803.612693E-621.9997273
GATTTCG500.00257965721.99972541
CGCAATA2600.021.15358236
TTAACGG1057.7367076E-820.95212235
CGCGCCA755.4693628E-520.5330810
GCTACGC755.4693628E-520.5330832
TAGCGGC2900.020.48250430
CTAGCGG2900.020.48250429
TAGTACT654.9300096E-420.307444
TCTAGCG2950.020.13534428
GATATAT1101.2586315E-720.0079651
TCTAATG550.004478717720.002492
TAGACCC550.00448226719.999754
AACGGAC1001.1223256E-619.79975315
CGCCGGT3150.019.5553137
Produced by FastQC (version 0.11.5)