##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062500_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	730711
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214403505626713	32.0	32.0	32.0	32.0	32.0
2	31.338031040999795	32.0	32.0	32.0	32.0	32.0
3	31.461488878640118	32.0	32.0	32.0	32.0	32.0
4	31.5879273748445	32.0	32.0	32.0	32.0	32.0
5	31.50343569482326	32.0	32.0	32.0	32.0	32.0
6	35.0906637507852	36.0	36.0	36.0	36.0	36.0
7	35.115201495529696	36.0	36.0	36.0	36.0	36.0
8	35.047084278189324	36.0	36.0	36.0	36.0	36.0
9	35.19261650638898	36.0	36.0	36.0	36.0	36.0
10	35.05371754359795	36.0	36.0	36.0	36.0	36.0
11	35.20101517563031	36.0	36.0	36.0	36.0	36.0
12	35.09861354215278	36.0	36.0	36.0	36.0	36.0
13	35.1544139885673	36.0	36.0	36.0	36.0	36.0
14	35.103656575581866	36.0	36.0	36.0	36.0	36.0
15	35.06141689395671	36.0	36.0	36.0	36.0	36.0
16	35.068441558974754	36.0	36.0	36.0	36.0	36.0
17	35.036201726811285	36.0	36.0	36.0	36.0	36.0
18	35.062742999626394	36.0	36.0	36.0	36.0	36.0
19	35.03798218447512	36.0	36.0	36.0	36.0	36.0
20	35.028847246038445	36.0	36.0	36.0	36.0	36.0
21	35.01131363836045	36.0	36.0	36.0	36.0	36.0
22	34.988501610075666	36.0	36.0	36.0	36.0	36.0
23	34.93399168754816	36.0	36.0	36.0	32.0	36.0
24	34.91056245218698	36.0	36.0	36.0	32.0	36.0
25	34.87163598194088	36.0	36.0	36.0	32.0	36.0
26	34.811252328211836	36.0	36.0	36.0	32.0	36.0
27	34.79667611408615	36.0	36.0	36.0	32.0	36.0
28	34.765832182627605	36.0	36.0	36.0	32.0	36.0
29	34.730342091469815	36.0	36.0	36.0	32.0	36.0
30	34.71688259790807	36.0	36.0	36.0	32.0	36.0
31	34.7103246016551	36.0	36.0	36.0	32.0	36.0
32	34.666788922022526	36.0	36.0	36.0	32.0	36.0
33	34.64350338232215	36.0	36.0	36.0	32.0	36.0
34	34.607420717629815	36.0	36.0	36.0	32.0	36.0
35	34.56595972963319	36.0	36.0	36.0	32.0	36.0
36	34.52993591173528	36.0	36.0	36.0	32.0	36.0
37	34.4982612825043	36.0	36.0	36.0	32.0	36.0
38	34.43815270332594	36.0	36.0	36.0	32.0	36.0
39	34.370629428050215	36.0	36.0	36.0	32.0	36.0
40	34.32519422863485	36.0	36.0	36.0	32.0	36.0
41	34.304938614582234	36.0	36.0	36.0	32.0	36.0
42	34.20179660631905	36.0	36.0	36.0	32.0	36.0
43	34.23992385498508	36.0	36.0	36.0	32.0	36.0
44	34.205176875673146	36.0	36.0	36.0	32.0	36.0
45	34.08964145879835	36.0	36.0	36.0	32.0	36.0
46	34.15687050010195	36.0	36.0	36.0	32.0	36.0
47	34.09734354621732	36.0	36.0	36.0	32.0	36.0
48	34.066891014368196	36.0	36.0	36.0	32.0	36.0
49	34.087048094253404	36.0	36.0	36.0	32.0	36.0
50	33.51735501449958	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	16.0
22	62.0
23	217.0
24	534.0
25	1335.0
26	2823.0
27	5433.0
28	8985.0
29	13339.0
30	18889.0
31	27046.0
32	40266.0
33	61265.0
34	131832.0
35	418661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61982701865182	17.078725812655097	11.145648981178457	26.155798187514627
2	16.311613164579136	20.22395331523167	36.02166725059122	27.442766269597968
3	19.186460254985576	23.84386102246041	28.090153659190815	28.879525063363204
4	12.245247075439163	15.233307969804628	35.34654061540314	37.17490433935306
5	14.388314942569636	36.69125002908126	33.33711959995128	15.583315428397821
6	35.63019873930144	34.61405818482399	16.20213875347951	13.553604322395055
7	30.71583704090947	30.097398287421427	20.354011367011037	18.832753304658066
8	29.02843942406779	32.2953944856448	19.23688024403629	19.439285846251117
9	27.23662296037695	13.793688612871573	17.96264186525179	41.007046561499685
10	15.759582105647787	26.45368688852364	31.47455012994193	26.312180875886636
11	38.65495387369288	20.67712132429921	21.03458138716948	19.633343414838425
12	24.260891104691183	23.51476849260515	28.67303215635183	23.55130824635184
13	30.033761637637863	18.6629187188916	25.307953486398866	25.995366157071675
14	23.791758985426522	19.258366166651385	24.40951347386313	32.54036137405896
15	25.786117904342483	26.62297406224896	22.521489343940353	25.069418689468204
16	26.657050461810485	25.29016259506152	23.635472847678493	24.4173140954495
17	24.327127961670207	25.5660582638006	25.389381027519768	24.71743274700942
18	25.974290793487437	23.748923993206617	26.443559765762387	23.83322544754356
19	25.554699463946758	25.5671530878829	24.586874975195393	24.29127247297495
20	25.218451617671008	23.75070308234035	25.796655586134598	25.23418971385404
21	26.749699949774946	24.20368654639112	23.757956291885574	25.28865721194836
22	25.378706492717367	24.4977836654984	24.81624062043681	25.30726922134743
23	24.14265010380301	23.85033207382946	25.1509830835994	26.85603473876813
24	24.404313059472212	25.593155159837472	24.775458423371212	25.227073357319103
25	25.441522024439212	24.07709751187542	24.560736050230528	25.92064441345484
26	24.35449856372766	24.919564643203675	25.440837759387776	25.285099033680897
27	25.833058486871007	24.60466586653273	24.861949525872745	24.700326120723513
28	24.85086443203948	23.896862097327123	25.65651810360047	25.59575536703293
29	24.18685362612579	24.9053319301338	25.773527427396058	25.134287016344352
30	24.91983834922425	24.54239774685204	25.81896262681142	24.71880127711229
31	24.749182645396058	24.70935841940247	24.66775510427515	25.873703830926313
32	24.673913489738077	24.841147868309086	24.802965878438947	25.681972763513894
33	24.31672713288838	24.15195610850254	25.313290753800068	26.218026004809015
34	24.844979752597126	24.95651495598123	25.82388933518176	24.374615956239882
35	26.161095152529523	24.18042153464229	25.417572747638946	24.240910565189246
36	24.540208098687444	25.337240030600334	24.922985968460857	25.19956590225137
37	26.10224835810601	24.696357383425184	24.965273548639612	24.236120709829194
38	24.86824476434596	24.488067101768003	24.984432970079826	25.65925516380621
39	25.331902763199132	24.47232900558497	24.822125299879158	25.373642931336736
40	25.79515020302144	24.801871054356646	24.81062964701503	24.59234909560688
41	24.01606106928731	25.149340847475955	25.77393798642692	25.060660096809816
42	26.400587920532192	25.34681974132044	24.789006871389645	23.46358546675772
43	25.24910669197535	23.56773060758631	25.136750370529526	26.046412329908815
44	24.655164627328723	24.622730463890647	25.413467157330327	25.308637751450302
45	24.91628017095678	24.83786339606219	25.03972158623587	25.206134846745154
46	24.465109277278263	24.47441529471336	25.58580011221962	25.47467531578875
47	25.247190749831326	24.089414282801272	25.90572743533353	24.75766753203387
48	25.781601755003003	25.772980015354907	23.96706769160448	24.47835053803761
49	24.32863334478337	25.085841050702673	25.629421207563592	24.95610439695037
50	24.36968924786954	26.068993076606212	24.013871421122715	25.547446254401535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	13.0
2	10.0
3	57.5
4	105.0
5	110.0
6	115.0
7	103.5
8	92.0
9	127.5
10	163.0
11	250.5
12	338.0
13	600.5
14	863.0
15	1180.5
16	1498.0
17	1814.0
18	2130.0
19	2236.0
20	2342.0
21	2756.5
22	3171.0
23	3415.0
24	3659.0
25	4096.5
26	4534.0
27	5678.0
28	6822.0
29	7796.5
30	8771.0
31	9558.5
32	10346.0
33	11906.5
34	13467.0
35	15592.5
36	17718.0
37	21447.0
38	25176.0
39	27166.5
40	29157.0
41	32345.5
42	35534.0
43	36800.0
44	38066.0
45	43791.5
46	49517.0
47	54519.0
48	59521.0
49	59558.0
50	59595.0
51	55124.5
52	50654.0
53	49630.5
54	48607.0
55	50220.0
56	51833.0
57	50489.5
58	49146.0
59	45495.0
60	41844.0
61	36968.0
62	32092.0
63	28123.0
64	24154.0
65	20705.5
66	17257.0
67	15091.5
68	12926.0
69	12176.5
70	11427.0
71	8680.0
72	5933.0
73	5462.0
74	4991.0
75	3520.0
76	2049.0
77	1945.5
78	1842.0
79	1565.0
80	1288.0
81	1020.5
82	753.0
83	664.5
84	576.0
85	462.5
86	349.0
87	268.0
88	187.0
89	116.5
90	46.0
91	32.5
92	19.0
93	10.5
94	2.0
95	4.0
96	6.0
97	4.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015327537152170969
2	0.003147619236606538
3	4.1055903086172237E-4
4	4.1055903086172237E-4
5	0.0
6	6.842650514362039E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.3685301028724077E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	730711.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.72930101693545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74794675307734	43.6454938333171
2	12.360294606875662	13.529405683954037
3	3.474604773586464	5.7048807170548335
4	1.4501765632549173	3.174685986323332
5	0.7537094636692381	2.0624996058233354
6	0.4427803848189975	1.4539836579092058
7	0.2832510233458229	1.0851491364033987
8	0.21204112690878324	0.9283890130048809
9	0.14977541414136508	0.7377393352931046
>10	0.9194682111451804	9.598154566585995
>50	0.09845507667978724	3.7718621531509067
>100	0.0987059924793031	10.661813182328682
>500	0.007283648299909319	2.623528523497841
>1k	0.0015069617172226177	1.022414605353502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1703	0.23306067651917106	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1459	0.1996685420090843	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1111	0.1520436944291245	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1081	0.14793810412050729	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1076	0.14725383906907108	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1008	0.1379478343695387	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	991	0.1356213331946556	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	850	0.11632505874415466	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	803	0.10989296726065434	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	801	0.10961926124007987	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	736	0.10072381557140922	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	732	0.10017640353026025	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	732	0.10017640353026025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3685301028724077E-4
2	0.0	0.0	0.0	0.0	1.3685301028724077E-4
3	0.0	0.0	0.0	0.0	1.3685301028724077E-4
4	0.0	0.0	0.0	0.0	1.3685301028724077E-4
5	0.0	0.0	0.0	0.0	2.7370602057448154E-4
6	0.0	0.0	0.0	0.0	4.1055903086172237E-4
7	0.0	0.0	0.0	0.0	4.1055903086172237E-4
8	0.0	0.0	0.0	0.0	4.1055903086172237E-4
9	0.0	0.0	0.0	1.3685301028724077E-4	4.1055903086172237E-4
10	0.0	0.0	0.0	1.3685301028724077E-4	4.1055903086172237E-4
11	0.0	0.0	0.0	1.3685301028724077E-4	4.1055903086172237E-4
12	0.0	0.0	0.0	1.3685301028724077E-4	4.1055903086172237E-4
13	0.0	0.0	0.0	1.3685301028724077E-4	4.1055903086172237E-4
14	0.0	0.0	0.0	1.3685301028724077E-4	5.474120411489631E-4
15	0.0	0.0	0.0	1.3685301028724077E-4	5.474120411489631E-4
16	0.0	0.0	0.0	1.3685301028724077E-4	5.474120411489631E-4
17	0.0	0.0	0.0	1.3685301028724077E-4	5.474120411489631E-4
18	0.0	0.0	0.0	2.7370602057448154E-4	5.474120411489631E-4
19	0.0	0.0	0.0	4.1055903086172237E-4	5.474120411489631E-4
20	0.0	0.0	0.0	6.842650514362039E-4	5.474120411489631E-4
21	0.0	0.0	0.0	9.579710720106855E-4	6.842650514362039E-4
22	0.0	0.0	0.0	0.002600207195457575	6.842650514362039E-4
23	0.0	0.0	0.0	0.005063561380627909	6.842650514362039E-4
24	0.0	0.0	0.0	0.008211180617234448	6.842650514362039E-4
25	0.0	0.0	0.0	0.010674534802404781	6.842650514362039E-4
26	0.0	0.0	0.0	0.01423271306987304	6.842650514362039E-4
27	0.0	0.0	0.0	0.017927744347628543	6.842650514362039E-4
28	0.0	0.0	0.0	0.022170187666533007	6.842650514362039E-4
29	0.0	0.0	0.0	0.031749898386639865	6.842650514362039E-4
30	0.0	0.0	0.0	0.04488778737421498	6.842650514362039E-4
31	0.0	0.0	0.0	0.07184783040080141	6.842650514362039E-4
32	0.0	0.0	0.0	0.10893499618864366	6.842650514362039E-4
33	0.0	0.0	0.0	0.1460221619764859	6.842650514362039E-4
34	0.0	0.0	0.0	0.19090994935070088	8.211180617234447E-4
35	0.0	0.0	0.0	0.2419561221878417	8.211180617234447E-4
36	0.0	0.0	0.0	0.31530933570180275	8.211180617234447E-4
37	0.0	0.0	0.0	0.4208230066332654	8.211180617234447E-4
38	0.0	0.0	0.0	0.5442644219123566	8.211180617234447E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTGTC	35	3.216861E-4	31.42818	31
CTACGCT	60	9.747031E-6	25.666346	33
ATTTCGT	55	1.5929117E-4	23.9997	42
TACGCTC	65	1.8122926E-5	23.692013	34
CGAGCCG	355	0.0	22.30958	15
CCTAAGC	80	3.612693E-6	21.999727	3
GATTTCG	50	0.002579657	21.999725	41
CGCAATA	260	0.0	21.153582	36
TTAACGG	105	7.7367076E-8	20.952122	35
CGCGCCA	75	5.4693628E-5	20.53308	10
GCTACGC	75	5.4693628E-5	20.53308	32
TAGCGGC	290	0.0	20.482504	30
CTAGCGG	290	0.0	20.482504	29
TAGTACT	65	4.9300096E-4	20.30744	4
TCTAGCG	295	0.0	20.135344	28
GATATAT	110	1.2586315E-7	20.007965	1
TCTAATG	55	0.0044787177	20.00249	2
TAGACCC	55	0.004482267	19.99975	4
AACGGAC	100	1.1223256E-6	19.799753	15
CGCCGGT	315	0.0	19.555313	7
>>END_MODULE