FastQCFastQC Report
Thu 2 Feb 2017
SRR4062499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946332
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36420.18712121056428194No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25250.12973120721439096No Hit
GCGCAAGACGGACCAGAGCGAAAGC24560.12618607719546304No Hit
TATCAACGCAGAGTACTTTTTTTTT23830.12243543239282917No Hit
GTACATGGAAGCAGTGGTATCAACG23740.12197302412949075No Hit
GAATAACGCCGCCGCATCGCCAGTC23740.12197302412949075No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG23450.12048304194762251No Hit
GAATAGGACCGCGGTTCTATTTTGT23270.11955822542094566No Hit
GAACTACGACGGTATCTGATCGTCT22830.11729756280018003No Hit
GATTAAGAGGGACGGCCGGGGGCAT22740.1168351545368416No Hit
GGGTAGGCACACGCTGAGCCAGTCA22030.11318726712606071No Hit
GTATCTGATCGTCTTCGAACCTCCG21640.11118349798492755No Hit
GTCCTATTCCATTATTCCTAGCTGC21480.11036143885010369No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19530.10034259314443784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1152.5465852E-1114.0336531
AACCGCG2300.013.2183417
ACGGTAT4050.012.4339519
AGAGCGA4450.012.38386715
TAACGAA2850.012.33485413
AGAATTT4800.012.27297817
GAATTTC4850.012.14676518
TAGACCG550.003070229212.090225
CAAGACG4450.011.9528394
TCGCCAG3350.011.91252917
TAACTTT2000.011.8758536
GATAACG3050.011.83691511
CGATAAC2900.011.79455710
CGCAAGA4350.011.786672
GCATATA1052.7458373E-711.7536751
TTATACG658.061824E-411.6859292
CCGACCA3200.011.579859
GGATAAG1406.87578E-1011.5276431
AAGACGG4700.011.5206215
ATCGCCA3550.011.50905316
Produced by FastQC (version 0.11.5)