##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062499_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1946332 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.157938625064993 32.0 32.0 32.0 32.0 32.0 2 30.78074192892066 32.0 32.0 32.0 32.0 32.0 3 30.765038030510723 32.0 32.0 32.0 32.0 32.0 4 30.856586646060386 32.0 32.0 32.0 32.0 32.0 5 30.616117907941707 32.0 32.0 32.0 32.0 32.0 6 34.25335451505704 36.0 36.0 36.0 32.0 36.0 7 34.14557742461204 36.0 36.0 36.0 32.0 36.0 8 34.143566976240436 36.0 36.0 36.0 32.0 36.0 9 34.32504475084415 36.0 36.0 36.0 32.0 36.0 10 33.956876832934974 36.0 36.0 36.0 32.0 36.0 11 34.30684025130348 36.0 36.0 36.0 32.0 36.0 12 34.06682518706983 36.0 36.0 36.0 32.0 36.0 13 34.2067920580867 36.0 36.0 36.0 32.0 36.0 14 34.10196461857484 36.0 36.0 36.0 32.0 36.0 15 34.0252207742564 36.0 36.0 36.0 32.0 36.0 16 34.02440436677812 36.0 36.0 36.0 32.0 36.0 17 33.946156668029914 36.0 36.0 36.0 32.0 36.0 18 33.96782511924995 36.0 36.0 36.0 32.0 36.0 19 33.952318001245416 36.0 36.0 36.0 32.0 36.0 20 33.94446836408177 36.0 36.0 36.0 32.0 36.0 21 33.92382800056722 36.0 36.0 36.0 32.0 36.0 22 33.8732934566148 36.0 36.0 36.0 32.0 36.0 23 33.83780053968182 36.0 36.0 36.0 32.0 36.0 24 33.80080171317124 36.0 36.0 36.0 32.0 36.0 25 33.33379968063003 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 16.0 4 75.0 5 182.0 6 341.0 7 80.0 8 262.0 9 349.0 10 241.0 11 72.0 12 133.0 13 104.0 14 234.0 15 337.0 16 485.0 17 777.0 18 1090.0 19 1597.0 20 2611.0 21 3784.0 22 6201.0 23 8792.0 24 12562.0 25 17489.0 26 24083.0 27 31338.0 28 42278.0 29 56338.0 30 72351.0 31 99764.0 32 143926.0 33 205612.0 34 448070.0 35 764758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99945430638076 17.214013124393997 10.668413023271109 26.118119545954134 2 17.528387779638894 19.814670187950224 35.91138902304933 26.745553009361544 3 19.370259748200496 23.51385832411466 28.004229713739015 29.111652213945828 4 12.735117047232036 14.826222526427864 35.57746658362675 36.861193842713355 5 14.958887690772112 36.14542419330135 33.24206972902573 15.653618386900803 6 34.64659574850869 35.13286231614009 16.504791987396136 13.715749947955086 7 30.587546971727765 30.157327332468576 20.489827269488288 18.765298426315375 8 28.870075788352107 31.61425384624087 19.39360062037636 20.122069745030664 9 27.62750670357102 13.82766448305619 18.35832194214821 40.18650687122458 10 16.27095209142843 25.827873161467803 30.928234360967 26.972940386136766 11 38.19897709602469 20.968708479568413 21.552379977500845 19.279934446906058 12 24.811413890370805 23.56417072271064 28.14240302663793 23.482012360280624 13 29.46551338338439 18.86569805274136 25.237922546893927 26.43086601698032 14 23.74850948732537 19.44703651614589 24.383038351572715 32.42141564495603 15 25.88159230030537 26.301614921655958 22.01153081940416 25.805261958634514 16 26.567087409601992 25.304447119093908 22.92277464020821 25.205690831095882 17 24.358519964958507 25.34649258403246 24.70265913092772 25.59232832008132 18 25.54222681337314 24.14504104255709 25.621811716626297 24.690920427443466 19 25.925264761896923 24.721708057630792 24.771609746159882 24.5814174343124 20 25.901522466110123 23.905912345416734 24.475711889350762 25.716853299122384 21 27.38360787299497 23.616361260584608 23.79893613737615 25.20109472904427 22 26.252854471602443 23.80050446578802 24.331418174558337 25.615222888051196 23 24.845896798059336 23.634216801763678 24.98686759292368 26.533018807253306 24 24.989234765456793 24.879293039201915 24.5608890411713 25.57058315416999 25 25.26652347819377 24.117808959139843 24.676295448407135 25.939372114259257 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 125.0 1 125.0 2 485.5 3 846.0 4 846.0 5 846.0 6 2416.5 7 3987.0 8 3987.0 9 3987.0 10 4168.0 11 4349.0 12 4349.0 13 4349.0 14 4694.5 15 5040.0 16 5040.0 17 5040.0 18 8276.0 19 11512.0 20 11512.0 21 11512.0 22 18692.0 23 25872.0 24 25872.0 25 25872.0 26 39233.5 27 52595.0 28 52595.0 29 52595.0 30 64280.0 31 75965.0 32 75965.0 33 75965.0 34 95057.0 35 114149.0 36 114149.0 37 114149.0 38 129519.0 39 144889.0 40 144889.0 41 144889.0 42 166861.0 43 188833.0 44 188833.0 45 188833.0 46 219702.5 47 250572.0 48 250572.0 49 250572.0 50 254422.5 51 258273.0 52 258273.0 53 258273.0 54 241365.0 55 224457.0 56 224457.0 57 224457.0 58 207392.5 59 190328.0 60 190328.0 61 190328.0 62 172770.0 63 155212.0 64 155212.0 65 155212.0 66 131795.5 67 108379.0 68 108379.0 69 108379.0 70 83108.5 71 57838.0 72 57838.0 73 57838.0 74 46477.0 75 35116.0 76 35116.0 77 35116.0 78 28317.5 79 21519.0 80 21519.0 81 21519.0 82 15444.5 83 9370.0 84 9370.0 85 9370.0 86 6859.0 87 4348.0 88 4348.0 89 4348.0 90 2963.5 91 1579.0 92 1579.0 93 1579.0 94 958.0 95 337.0 96 337.0 97 337.0 98 589.5 99 842.0 100 842.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009505058746400922 2 0.0036992661067073856 3 0.005189248288575639 4 0.014386034859417612 5 0.035245785405573146 6 0.04608669024606285 7 0.0657133520899826 8 0.08765205525059445 9 0.09484507268030326 10 0.11580758061831178 11 0.11606447409794422 12 0.12973120721439096 13 0.11904443846168075 14 0.12176750934578479 15 0.11051557493788317 16 0.11056695363380964 17 0.12079131412318146 18 0.1282412250325227 19 0.1289605267754936 20 0.11894168106982776 21 0.12700813633028693 22 0.14848443122756036 23 0.13702698203595276 24 0.13230014201071552 25 0.12880639068771413 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1946332.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.93262551446434 #Duplication Level Percentage of deduplicated Percentage of total 1 75.79272669339471 34.0556620523445 2 13.917831785750273 12.50729447204851 3 4.3579426760585465 5.874414188305239 4 1.8457590892610811 3.3173920779074764 5 0.9943316607651784 2.233896607516858 6 0.603207487447918 1.6262217684610947 7 0.4023088297121937 1.2653754390634284 8 0.29308876025896846 1.0535398005771879 9 0.22575147506620669 0.9129245839639017 >10 1.2975483580226836 10.819968263367933 >50 0.12582689061863325 3.9791816947856744 >100 0.11783604278549496 11.393522071725723 >500 0.018375289499123353 5.650180977833323 >1k 0.007464961359018862 5.3104260020992164 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3642 0.18712121056428194 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 2525 0.12973120721439096 No Hit GCGCAAGACGGACCAGAGCGAAAGC 2456 0.12618607719546304 No Hit TATCAACGCAGAGTACTTTTTTTTT 2383 0.12243543239282917 No Hit GTACATGGAAGCAGTGGTATCAACG 2374 0.12197302412949075 No Hit GAATAACGCCGCCGCATCGCCAGTC 2374 0.12197302412949075 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2345 0.12048304194762251 No Hit GAATAGGACCGCGGTTCTATTTTGT 2327 0.11955822542094566 No Hit GAACTACGACGGTATCTGATCGTCT 2283 0.11729756280018003 No Hit GATTAAGAGGGACGGCCGGGGGCAT 2274 0.1168351545368416 No Hit GGGTAGGCACACGCTGAGCCAGTCA 2203 0.11318726712606071 No Hit GTATCTGATCGTCTTCGAACCTCCG 2164 0.11118349798492755 No Hit GTCCTATTCCATTATTCCTAGCTGC 2148 0.11036143885010369 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1953 0.10034259314443784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5413608777947442E-4 2 0.0 0.0 0.0 0.0 1.5413608777947442E-4 3 0.0 0.0 0.0 0.0 1.5413608777947442E-4 4 0.0 0.0 0.0 5.137869592649147E-5 1.5413608777947442E-4 5 0.0 0.0 0.0 5.137869592649147E-5 1.5413608777947442E-4 6 0.0 0.0 0.0 5.137869592649147E-5 1.5413608777947442E-4 7 0.0 0.0 0.0 5.137869592649147E-5 1.5413608777947442E-4 8 0.0 0.0 0.0 5.137869592649147E-5 1.5413608777947442E-4 9 0.0 0.0 0.0 1.0275739185298294E-4 1.5413608777947442E-4 10 0.0 0.0 0.0 1.0275739185298294E-4 1.5413608777947442E-4 11 0.0 0.0 0.0 1.0275739185298294E-4 1.5413608777947442E-4 12 0.0 0.0 0.0 1.0275739185298294E-4 2.0551478370596588E-4 13 0.0 0.0 0.0 1.0275739185298294E-4 2.5689347963245735E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAC 115 2.5465852E-11 14.033653 1 AACCGCG 230 0.0 13.218341 7 ACGGTAT 405 0.0 12.433951 9 AGAGCGA 445 0.0 12.383867 15 TAACGAA 285 0.0 12.334854 13 AGAATTT 480 0.0 12.272978 17 GAATTTC 485 0.0 12.146765 18 TAGACCG 55 0.0030702292 12.09022 5 CAAGACG 445 0.0 11.952839 4 TCGCCAG 335 0.0 11.912529 17 TAACTTT 200 0.0 11.875853 6 GATAACG 305 0.0 11.836915 11 CGATAAC 290 0.0 11.794557 10 CGCAAGA 435 0.0 11.78667 2 GCATATA 105 2.7458373E-7 11.753675 1 TTATACG 65 8.061824E-4 11.685929 2 CCGACCA 320 0.0 11.57985 9 GGATAAG 140 6.87578E-10 11.527643 1 AAGACGG 470 0.0 11.520621 5 ATCGCCA 355 0.0 11.509053 16 >>END_MODULE