FastQCFastQC Report
Thu 2 Feb 2017
SRR4062499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946332
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26720.1372838755155852No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24760.12721365111399288No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24190.12428506544618287No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23470.12058579933947547No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22790.11709204801647406No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22400.1150882788753409No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG22170.1139065688690316No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA21090.10835766970897051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA1252.2902896E-819.35987711
ATACGAA7900.017.54419140
GTATCAA39550.017.5256141
GTCGTAC1651.6570993E-917.3367861
GTATTAG6700.017.0780281
CGAATGC7950.016.88039643
ATACCGT5100.016.8234216
TACCGTC5250.016.7617977
ATTACAC2500.016.7203243
ACCGTCG5300.016.6036668
GGCGTTA5450.016.55035442
ATAACCG1602.1314918E-816.49989310
CGCAATA8000.016.49989336
CGTCGTA4950.016.4443410
TAACGGC3900.016.3588736
TTAACGG3900.016.3588735
CCGTCGT5250.016.3427529
GCAATAC8350.016.33522437
GTCCTAT6650.016.2137551
TATTACA2850.016.2112542
Produced by FastQC (version 0.11.5)