##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1946332
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24862870260572	32.0	32.0	32.0	32.0	32.0
2	31.346568827928635	32.0	32.0	32.0	32.0	32.0
3	31.451488749093166	32.0	32.0	32.0	32.0	32.0
4	31.57089386600025	32.0	32.0	32.0	32.0	32.0
5	31.48398115018404	32.0	32.0	32.0	32.0	32.0
6	35.07200159068442	36.0	36.0	36.0	36.0	36.0
7	35.09035251950849	36.0	36.0	36.0	36.0	36.0
8	35.02280854448265	36.0	36.0	36.0	36.0	36.0
9	35.173204776985635	36.0	36.0	36.0	36.0	36.0
10	35.02394401366262	36.0	36.0	36.0	36.0	36.0
11	35.18252281727886	36.0	36.0	36.0	36.0	36.0
12	35.072128496063364	36.0	36.0	36.0	36.0	36.0
13	35.13152689263702	36.0	36.0	36.0	36.0	36.0
14	35.08815916297939	36.0	36.0	36.0	36.0	36.0
15	35.04690772180697	36.0	36.0	36.0	36.0	36.0
16	35.054067856871285	36.0	36.0	36.0	36.0	36.0
17	35.02536052430931	36.0	36.0	36.0	36.0	36.0
18	35.04522815223713	36.0	36.0	36.0	36.0	36.0
19	35.028332268081705	36.0	36.0	36.0	36.0	36.0
20	35.01923001831137	36.0	36.0	36.0	36.0	36.0
21	35.015687971014195	36.0	36.0	36.0	36.0	36.0
22	34.99778455063165	36.0	36.0	36.0	36.0	36.0
23	34.958102728619785	36.0	36.0	36.0	32.0	36.0
24	34.93384222219025	36.0	36.0	36.0	32.0	36.0
25	34.901942217463414	36.0	36.0	36.0	32.0	36.0
26	34.85922494209621	36.0	36.0	36.0	32.0	36.0
27	34.838092884461645	36.0	36.0	36.0	32.0	36.0
28	34.82795072988576	36.0	36.0	36.0	32.0	36.0
29	34.804759927905415	36.0	36.0	36.0	32.0	36.0
30	34.79537817802924	36.0	36.0	36.0	32.0	36.0
31	34.79044479564637	36.0	36.0	36.0	32.0	36.0
32	34.75509214255327	36.0	36.0	36.0	32.0	36.0
33	34.73074531991459	36.0	36.0	36.0	32.0	36.0
34	34.719758499577665	36.0	36.0	36.0	32.0	36.0
35	34.68931353951947	36.0	36.0	36.0	32.0	36.0
36	34.66166871838926	36.0	36.0	36.0	32.0	36.0
37	34.661957980447326	36.0	36.0	36.0	32.0	36.0
38	34.62075534903603	36.0	36.0	36.0	32.0	36.0
39	34.61302850695565	36.0	36.0	36.0	32.0	36.0
40	34.60487059761644	36.0	36.0	36.0	32.0	36.0
41	34.568579769535724	36.0	36.0	36.0	32.0	36.0
42	34.539843151117076	36.0	36.0	36.0	32.0	36.0
43	34.537521347848156	36.0	36.0	36.0	32.0	36.0
44	34.500029799643634	36.0	36.0	36.0	32.0	36.0
45	34.45669649371227	36.0	36.0	36.0	32.0	36.0
46	34.47136819412104	36.0	36.0	36.0	32.0	36.0
47	34.444030617592475	36.0	36.0	36.0	32.0	36.0
48	34.40201568899859	36.0	36.0	36.0	32.0	36.0
49	34.39538064420664	36.0	36.0	36.0	32.0	36.0
50	33.860207302762326	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	52.0
22	157.0
23	480.0
24	1250.0
25	2973.0
26	6120.0
27	11412.0
28	19429.0
29	30399.0
30	44436.0
31	64408.0
32	97611.0
33	162675.0
34	361131.0
35	1143786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44755612719166	17.81646198931077	11.168760029824194	26.567221853673377
2	16.949789135331546	20.283341691192184	35.49837843836833	27.268490735107942
3	18.75934134194719	23.72167797603787	28.427479331518285	29.091501350496657
4	12.519202807334832	15.322581473850786	35.75364917562798	36.404566543186405
5	14.788741078089451	36.25465747878573	33.12682522817279	15.829776214952023
6	34.93215929105329	35.293712660379256	16.2192420172592	13.55488603130825
7	30.637301999147116	30.19755128883591	20.411132747273072	18.7540139647439
8	28.87457021720857	31.81738778379023	19.186089526350077	20.12195247265112
9	27.68309825867324	13.56690431026156	18.245396982631945	40.50460044843326
10	16.24044613149247	25.82596391571428	30.903463540649796	27.030126412143456
11	38.427668044300766	20.814845565915785	21.52176504316838	19.23572134661507
12	24.755437407389902	23.50601027984948	28.07835456643574	23.660197746324883
13	29.704438913813263	18.6950633293806	25.27472188711895	26.325775869687185
14	23.838687335973514	19.328870922329795	24.274584192213865	32.55785754948282
15	26.045505083408177	26.336719531919528	21.796486930287333	25.821288454384966
16	26.771588814241355	25.249083917851628	22.755932698018633	25.223394569888384
17	24.59832135524669	25.296609211583636	24.462784355392607	25.64228507777707
18	25.793132929017247	24.033001563967503	25.532488804582158	24.64137670243309
19	26.16567985318024	24.615019431422798	24.606490567899	24.61281014749796
20	26.186334088942687	23.710857140508402	24.318256083751386	25.784552686797525
21	27.510928248623568	23.604040831677224	23.686246745159615	25.198784174539597
22	26.33363681016394	23.830929152888615	24.25125826426324	25.58417577268421
23	24.995838325629954	23.446308235182897	24.91270759562089	26.645145843566258
24	25.228892090352517	24.730056331602214	24.391470725446634	25.649580852598632
25	25.523548911490952	23.985373512843648	24.624062081905862	25.867015493759542
26	25.08549415002168	24.66495952386335	25.104504267514482	25.145042058600488
27	26.08599149579825	24.19864647963451	24.169257865564557	25.54610415900268
28	25.064171991212188	23.928445917757095	25.201764138903332	25.805617952127385
29	24.941685180123432	24.33356693513748	25.052457648540948	25.67229023619814
30	25.144271378161587	24.4755776506783	25.27631462669267	25.10383634446744
31	25.510858373597106	24.353964277420296	24.074001763316843	26.06117558566575
32	25.256276935281342	24.30279109627751	24.175371930379814	26.265560038061338
33	25.020859750546155	23.75571074205223	24.749426099966502	26.474003407435116
34	25.6175205463405	24.07980755595654	25.08236004957017	25.2203118481328
35	26.527951038157934	23.76742508472347	24.74757646691315	24.95704741020545
36	25.123976793270625	24.568984119872663	24.4117653103376	25.89527377651911
37	26.439887953339923	24.270011488276406	24.103904164346062	25.18619639403761
38	25.22097977117984	23.8873429610159	24.950470937126862	25.941206330677396
39	25.700034731998443	24.018615529108086	24.180612557364313	26.100737181529155
40	25.952766537260857	24.171672664273107	24.819660777298015	25.05590002116802
41	24.78004780273869	24.66598709778188	25.262185485312887	25.291779614166543
42	26.286265652519713	24.741462402097895	24.540879973200873	24.43139197218152
43	25.327385050443603	23.53149411302902	24.821921439918782	26.319199396608596
44	25.03077583885997	24.271090440890866	24.65411861902286	26.044015101226307
45	25.18337056576165	24.581982929942065	24.623599673642524	25.611046830653763
46	25.082090437947542	24.24656879695057	24.78445319367897	25.886887571422918
47	25.262660873201938	23.928715105498497	25.190782040670367	25.617841980629198
48	26.04016169903182	24.878283869350142	24.10714102218943	24.974413409428607
49	24.800303751006382	24.81936525698866	24.7487708925152	25.631560099489757
50	24.688966059729818	25.050466750002954	24.489205587333295	25.77136160293393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	12.0
2	17.0
3	55.5
4	94.0
5	135.0
6	176.0
7	160.5
8	145.0
9	217.0
10	289.0
11	441.0
12	593.0
13	1038.5
14	1484.0
15	2169.5
16	2855.0
17	3719.5
18	4584.0
19	5122.0
20	5660.0
21	6803.0
22	7946.0
23	8166.0
24	8386.0
25	10347.0
26	12308.0
27	15560.0
28	18812.0
29	20826.5
30	22841.0
31	24117.5
32	25394.0
33	28919.0
34	32444.0
35	38603.0
36	44762.0
37	56105.0
38	67448.0
39	73071.0
40	78694.0
41	87663.5
42	96633.0
43	97630.5
44	98628.0
45	109570.0
46	120512.0
47	129089.0
48	137666.0
49	142917.0
50	148168.0
51	142511.0
52	136854.0
53	134989.5
54	133125.0
55	136483.5
56	139842.0
57	138554.0
58	137266.0
59	125780.5
60	114295.0
61	102082.5
62	89870.0
63	79371.0
64	68872.0
65	59900.0
66	50928.0
67	45910.5
68	40893.0
69	37548.5
70	34204.0
71	27227.0
72	20250.0
73	18394.5
74	16539.0
75	12299.0
76	8059.0
77	7159.0
78	6259.0
79	5456.0
80	4653.0
81	3760.5
82	2868.0
83	2570.0
84	2272.0
85	1906.5
86	1541.0
87	1178.0
88	815.0
89	536.0
90	257.0
91	161.5
92	66.0
93	48.0
94	30.0
95	22.0
96	14.0
97	11.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013409839636814273
2	0.0030827217555894886
3	5.651656551914062E-4
4	1.0275739185298294E-4
5	0.0
6	4.1102956741193177E-4
7	1.0275739185298294E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5413608777947442E-4
47	5.137869592649147E-5
48	0.0
49	5.137869592649147E-5
50	5.137869592649147E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1946332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.5771771826544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14691021389534	36.22855039158175
2	13.85996271282143	13.188358034657771
3	4.318450172617772	6.1637900705130075
4	1.8329860400951155	3.488332064117495
5	0.965080908463736	2.295791268878812
6	0.5785614164723377	1.651579141353115
7	0.38208402215757625	1.272493545459658
8	0.27756875354942556	1.056475021439164
9	0.21203384008438328	0.907917442057198
>10	1.1898598216652259	10.38522309571066
>50	0.11456197690839783	3.8551364842862132
>100	0.1004606181751408	10.261152834568845
>500	0.01583842147348463	5.149980246335351
>1k	0.005641081620693156	4.095220359041083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2672	0.1372838755155852	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2476	0.12721365111399288	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2419	0.12428506544618287	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2347	0.12058579933947547	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2279	0.11709204801647406	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2240	0.1150882788753409	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2217	0.1139065688690316	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2109	0.10835766970897051	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.137869592649147E-5	0.0
5	0.0	0.0	0.0	5.137869592649147E-5	0.0
6	0.0	0.0	0.0	5.137869592649147E-5	5.137869592649147E-5
7	0.0	0.0	0.0	5.137869592649147E-5	5.137869592649147E-5
8	0.0	0.0	0.0	5.137869592649147E-5	5.137869592649147E-5
9	0.0	0.0	0.0	1.0275739185298294E-4	5.137869592649147E-5
10	0.0	0.0	0.0	1.0275739185298294E-4	1.0275739185298294E-4
11	0.0	0.0	0.0	1.0275739185298294E-4	1.0275739185298294E-4
12	0.0	0.0	0.0	1.0275739185298294E-4	3.5965087148544033E-4
13	0.0	0.0	0.0	1.0275739185298294E-4	4.1102956741193177E-4
14	0.0	0.0	0.0	1.0275739185298294E-4	4.1102956741193177E-4
15	0.0	0.0	0.0	1.0275739185298294E-4	4.1102956741193177E-4
16	0.0	0.0	0.0	2.5689347963245735E-4	4.6240826333842326E-4
17	0.0	0.0	0.0	2.5689347963245735E-4	4.6240826333842326E-4
18	0.0	0.0	0.0	3.0827217555894884E-4	4.6240826333842326E-4
19	0.0	0.0	0.0	3.5965087148544033E-4	4.6240826333842326E-4
20	0.0	0.0	0.0	7.193017429708807E-4	5.137869592649147E-4
21	0.0	0.0	0.0	8.73437830750355E-4	5.651656551914062E-4
22	0.0	0.0	0.0	0.0011303313103828124	6.165443511178977E-4
23	0.0	0.0	0.0	0.0015927395737212356	6.165443511178977E-4
24	0.0	0.0	0.0	0.0023120413166921165	6.165443511178977E-4
25	0.0	0.0	0.0	0.002723070884104048	6.165443511178977E-4
26	0.0	0.0	0.0	0.0036478874107808944	8.220591348238635E-4
27	0.0	0.0	0.0	0.0049323548089431814	8.73437830750355E-4
28	0.0	0.0	0.0	0.009813330921959872	8.73437830750355E-4
29	0.0	0.0	0.0	0.020705614458376063	8.73437830750355E-4
30	0.0	0.0	0.0	0.04069192717378125	8.73437830750355E-4
31	0.0	0.0	0.0	0.0825141856579453	8.73437830750355E-4
32	0.0	0.0	0.0	0.1312725680921857	8.73437830750355E-4
33	0.0	0.0	0.0	0.1783354535608519	8.73437830750355E-4
34	0.0	0.0	0.0	0.2337730664655362	9.248165266768465E-4
35	0.0	0.0	0.0	0.2971229985429002	9.248165266768465E-4
36	0.0	0.0	0.0	0.38883397077168746	9.248165266768465E-4
37	0.0	0.0	0.0	0.5150200479671505	9.248165266768465E-4
38	0.0	0.0	0.0	0.6842614723490135	9.248165266768465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	125	2.2902896E-8	19.359877	11
ATACGAA	790	0.0	17.544191	40
GTATCAA	3955	0.0	17.525614	1
GTCGTAC	165	1.6570993E-9	17.336786	1
GTATTAG	670	0.0	17.078028	1
CGAATGC	795	0.0	16.880396	43
ATACCGT	510	0.0	16.823421	6
TACCGTC	525	0.0	16.761797	7
ATTACAC	250	0.0	16.720324	3
ACCGTCG	530	0.0	16.603666	8
GGCGTTA	545	0.0	16.550354	42
ATAACCG	160	2.1314918E-8	16.499893	10
CGCAATA	800	0.0	16.499893	36
CGTCGTA	495	0.0	16.44434	10
TAACGGC	390	0.0	16.35887	36
TTAACGG	390	0.0	16.35887	35
CCGTCGT	525	0.0	16.342752	9
GCAATAC	835	0.0	16.335224	37
GTCCTAT	665	0.0	16.213755	1
TATTACA	285	0.0	16.211254	2
>>END_MODULE