FastQCFastQC Report
Thu 2 Feb 2017
SRR4062498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876868
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16420.18725737511233162No Hit
GTACATGGAAGCAGTGGTATCAACG15100.17220379806310643No Hit
TCCATGTACTCTGCGTTGATACCAC13100.14939534798852278No Hit
TATCAACGCAGAGTACTTTTTTTTT10810.12327967265312453No Hit
GCGCAAGACGGACCAGAGCGAAAGC9690.1105069406113577No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8970.10229589858450759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT508.741016E-515.1930572
CCCTTAA653.3941087E-614.6045381
CTCGTAG602.5643523E-514.25081310
CAAGACG1600.014.2475594
CGCATCG1152.5465852E-1114.0442813
GCGTTAT551.9709859E-413.8079281
GCATCGC1205.0931703E-1113.45910114
GCCGTTT852.690722E-713.41329815
TATTCCC852.6963426E-713.4109985
GAACCGC655.444265E-513.1545976
ACGCCCC801.9888867E-613.06697817
AAGACGG1750.013.0278275
CCGTCGT905.392194E-712.6673919
AACCGCG604.086885E-412.6673897
CGCAAGA1950.012.6608812
CTAGGAC905.425918E-712.6608813
CGCGGTC701.0892701E-412.21498410
TTAACCT550.003070542812.0888394
GTGCTAG550.003083702612.0819371
TAGGACA1750.011.9408114
Produced by FastQC (version 0.11.5)